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Flow cytometry analysis of TCR V delta 1 on TCR V delta1 positive PEER cells (top panel) or negative control HPB-ALL cells (bottom panel). Equal numbers of cells were stained with a FITC labeled TCR V delta 1 (TS8.2) monoclonal antibody (Product # TCR2730) or were left unstained. 5ul of primary antibody were used per test. All antibody incubations were performed for 30 minutes at room temperature.
|Tested species reactivity||Human|
|Published species reactivity||Primate, Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Human TCR V delta 1|
|Storage buffer||PBS with glycerol, 0.5% BSA|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1-2 µg/test|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
TCR2730 targets TCR V delta 1 in FACS applications and shows reactivity with Human samples.
The TCR2730 immunogen is human TCR V delta 1.
The ability of T cell receptors (TCR) to discriminate foreign from self-peptides presented by major histocompatibility complex (MHC) class II molecules is essential for an effective adaptive immune response. TCR recognition of self-peptides has been linked to autoimmune disease. Mutant self-peptides have been associated with tumors. Engagement of TCRs by a family of bacterial toxins know as superantigens has been responsible for toxic shock syndrome. Autoantibodies to V beta segments of T cell receptors have been isolated from patients with rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE). The autoantibodies block TH1-mediated inflammatory autodestructive reactions and are believed to be a method by which the immune system compensates for disease (ref5). T Cell and TCR Diversity Most human T cells express the TCR alpha-beta and either CD4 or CD8 molecule (single positive, SP). A small number of T cells lack both CD4 and CD8 (double negative, DN). Increased percentages of alpha-beta DN T cells have been identified in some autoimmune and immunodeficiency disorders. Gamma-delta T cells are primarily found within the epithelium. They show less TCR diversity and recognize antigens differently than alpha-beta T cells. Subsets of gamma-delta T cells have shown antitumor and immunoregulatory activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
γδ T cell activation by bispecific antibodies.
TCR2730 was used in flow cytometry to evaluate the therapeutic potential of a gammatheta T cell-targeting tribody for tumor immunotherapy
|Oberg HH,Kellner C,Gonnermann D,Peipp M,Peters C,Sebens S,Kabelitz D,Wesch D||Cellular immunology (296:41)||2015|
Characterization of γδT cells in naïve and HIV-infected chimpanzees and their responses to T-cell activators in vitro.
TCR2730 was used in flow cytometry to study the in vitro response of gammadelta T-cell subsets from HIV1-infected and control chimpanzees to T-cell activators
|Hodara VL,Parodi LM,Chavez D,Smith LM,Lanford R,Giavedoni LD||Journal of medical primatology (43:258)||2014|
Novel bispecific antibodies increase γδ T-cell cytotoxicity against pancreatic cancer cells.
TCR2730 was used in flow cytometry to study the cytotoxicity of gammadelta T-cells towards pancreatic cancer cells mediated by bispecific antibodies that selectively recruit to tumor antigens
|Oberg HH,Peipp M,Kellner C,Sebens S,Krause S,Petrick D,Adam-Klages S,Röcken C,Becker T,Vogel I,Weisner D,Freitag-Wolf S,Gramatzki M,Kabelitz D,Wesch D||Cancer research (74:1349)||2014|
V-chain preference of gamma/delta T-cell receptors in peripheral blood during term labor.
TCR2730 was used in flow cytometry to study the role of Vgamma and Vdelta chains in peripheral gamma/delta T cells during labor
|Barakonyi A,Miko E,Varga P,Szekeres-Bartho J||American journal of reproductive immunology (New York, N.Y. : 1989) (59:201)||2008|
Human immunodeficiency virus type 1 induces persistent changes in mucosal and blood gammadelta T cells despite suppressive therapy.
TCR2730 was used in flow cytometry to study the role of HIV-1 infection in mucosal and blood gamma-delta T Cells
|Poles MA,Barsoum S,Yu W,Yu J,Sun P,Daly J,He T,Mehandru S,Talal A,Markowitz M,Hurley A,Ho D,Zhang L||Journal of virology (77:10456)||2003|
gammadelta T-cell anergy in human immunodeficiency virus-infected persons with opportunistic infections and recovery after highly active antiretroviral therapy.
TCR2730 was used in flow cytometry to investigate gammadelta T cells in HIV-infected individuals
|Martini F,Urso R,Gioia C,De Felici A,Narciso P,Amendola A,Paglia MG,Colizzi V,Poccia F||Immunology (100:481)||2000|
CD1d-lipid antigen recognition by the γδ TCR.
TCR2730 was used in immunocytochemistry to study the mechanism by which the gamma delta TCR recognizes CD1d loaded with alpha-galactosylceramide
|Uldrich AP,Le Nours J,Pellicci DG,Gherardin NA,McPherson KG,Lim RT,Patel O,Beddoe T,Gras S,Rossjohn J,Godfrey DI||Nature immunology (14:1137)||2013|
Extensive expansion of primary human gamma delta T cells generates cytotoxic effector memory cells that can be labeled with Feraheme for cellular MRI.
TCR2730 was used in blocking or activating experiment to develop a method for the extensive expansion of gamma delta T-cells to be labelled with Feraheme for use in cellular MRI studies
|Siegers GM,Ribot EJ,Keating A,Foster PJ||Cancer immunology, immunotherapy : CII (62:571)||2013|
Maternal Foxp3 expressing CD4+ CD25+ and CD4+ CD25- regulatory T-cell populations are enriched in human early normal pregnancy decidua: a phenotypic study of paired decidual and peripheral blood samples.
TCR2730 was used in immunohistochemistry to identify relationship between T-cell populations and pregnancy
|Dimova T,Nagaeva O,Stenqvist AC,Hedlund M,Kjellberg L,Strand M,Dehlin E,Mincheva-Nilsson L||American journal of reproductive immunology (New York, N.Y. : 1989) (66 Suppl 1:44)||2011|
Flexible migration program regulates gamma delta T-cell involvement in humoral immunity.
TCR2730 was used in immunohistochemistry to investigate the migration of gamma delta T cells
|Brandes M,Willimann K,Lang AB,Nam KH,Jin C,Brenner MB,Morita CT,Moser B||Blood (102:3693)||2003|