Flow cytometry analysis of TCR V gamma 9 on IPP and IL2 stimmunlated human PBMCs. Equal numbers of cells were stained with a FITC labeled TCR V gamma 9 monoclonal antibody (7A5) (Product # TCR2720) or FITC labeled control. 5ul of primary antibody were used per test. All antibody incubations were performed for 30 minutes at room temperature. A representative 10,000 cells were acquired for each sample.
|Tested species reactivity||Human, Non-human primate|
|Published species reactivity||Non-human primate, Human, Rhesus monkey, Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Human TCR Vgamma9|
|Storage buffer||PBS with 0.5% BSA|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1-2 µg/test|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
TCR2720 targets TCR V gamma 9 in FACS applications and shows reactivity with Human, and Non-human primate samples.
The TCR2720 immunogen is human TCR Vgamma9.
The ability of T cell receptors (TCR) to discriminate foreign from self-peptides presented by major histocompatibility complex (MHC) class II molecules is essential for an effective adaptive immune response. TCR recognition of self-peptides has been linked to autoimmune disease. Mutant self-peptides have been associated with tumors. Engagement of TCRs by a family of bacterial toxins know as superantigens has been responsible for toxic shock syndrome. Autoantibodies to V beta segments of T cell receptors have been isolated from patients with rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE). The autoantibodies block TH1-mediated inflammatory autodestructive reactions and are believed to be a method by which the immune system compensates for disease (ref5). T Cell and TCR Diversity Most human T cells express the TCR alpha-beta and either CD4 or CD8 molecule (single positive, SP). A small number of T cells lack both CD4 and CD8 (double negative, DN). Increased percentages of alpha-beta DN T cells have been identified in some autoimmune and immunodeficiency disorders. Gamma-delta T cells are primarily found within the epithelium. They show less TCR diversity and recognize antigens differently than alpha-beta T cells. Subsets of gamma-delta T cells have shown antitumor and immunoregulatory activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
HMBPP-deficient Listeria mutant immunization alters pulmonary/systemic responses, effector functions, and memory polarization of V¿2V¿2 T cells.
TCR2720 was used in flow cytometry to examine the effect of HMBPP-deficient Listeria mutant immunization
|Frencher JT,Shen H,Yan L,Wilson JO,Freitag NE,Rizzo AN,Chen CY,Chen ZW||Journal of leukocyte biology (96:957)||2014|
|Rhesus monkey||Not Cited||
Multieffector-functional immune responses of HMBPP-specific V¿2V¿2 T cells in nonhuman primates inoculated with Listeria monocytogenes ¿actA prfA*.
TCR2720 was used in flow cytometry to study multi-effector functional Vgamma2Vdelta2 T cell responses against Listeria monocytogenes in nonhuman primates
|Ryan-Payseur B,Frencher J,Shen L,Chen CY,Huang D,Chen ZW||Journal of immunology (Baltimore, Md. : 1950) (189:1285)||2012|
|Non-human primate||Not Cited||
Phosphoantigen-activated V gamma 2V delta 2 T cells antagonize IL-2-induced CD4+CD25+Foxp3+ T regulatory cells in mycobacterial infection.
TCR2720 was used in flow cytometry to investigate the effect of phosphoantigen-activated V gamma 2V delta 2 T cells for interleukin-2-induced CD4+CD25+Foxp3+ T regulatory cells in mycobacterial infection
|Gong G,Shao L,Wang Y,Chen CY,Huang D,Yao S,Zhan X,Sicard H,Wang R,Chen ZW||Blood (113:837)||2009|
Human CD1-restricted T cell recognition of lipids from pollens.
TCR2720 was used in flow cytometry to study the immune response of pollen lipids
|Agea E,Russano A,Bistoni O,Mannucci R,Nicoletti I,Corazzi L,Postle AD,De Libero G,Porcelli SA,Spinozzi F||The Journal of experimental medicine (202:295)||2005|
|Not Applicable||Not Cited||
Recognition of nonpeptide antigens by human V gamma 9V delta 2 T cells requires contact with cells of human origin.
TCR2720 was used in flow cytometry to study gamma delta T cell activation
|Green AE,Lissina A,Hutchinson SL,Hewitt RE,Temple B,James D,Boulter JM,Price DA,Sewell AK||Clinical and experimental immunology (136:472)||2004|
Cytotoxic hepatosplenic gammadelta T-cell lymphoma following acute myeloid leukemia bearing two distinct gamma chains of the T-cell receptor. Biologic and clinical features.
TCR2720 was used in flow cytometry to study the expression of Vd1 and two distinct TCRg chains, Vg3 and Vg9 during hepatosplenic gd-T-cell lymphoma
|Weidmann E,Hinz T,Klein S,Schui DK,Harder S,Kriener S,Kabelitz D,Hoelzer D,Mitrou PS||Haematologica (85:1024)||2000|
Phosphoantigen/IL2 expansion and differentiation of V¿2V¿2 T cells increase resistance to tuberculosis in nonhuman primates.
TCR2720 was used in immunohistochemistry to study the increased Tuberculosis resistance of non-human primates following phoshoantigen/IL-2 mediated expansion and differentiation of VgammaVdelta2 T-cells
|Chen CY,Yao S,Huang D,Wei H,Sicard H,Zeng G,Jomaa H,Larsen MH,Jacobs WR,Wang R,Letvin N,Shen Y,Qiu L,Shen L,Chen ZW||PLoS pathogens (9:null)||2013|
Mechanisms of the antitumor activity of human V¿9V¿2 T cells in combination with zoledronic acid in a preclinical model of neuroblastoma.
TCR2720 was used in blocking or activating experiment to study the mechanisms underlying the therapautic activity of T-cell receptor Vgamma9Vdelta2 lymphocytes and zoledronic acid in a neuroblastoma model
|Di Carlo E,Bocca P,Emionite L,Cilli M,Cipollone G,Morandi F,Raffaghello L,Pistoia V,Prigione I||Molecular therapy : the journal of the American Society of Gene Therapy (21:1034)||2013|
Reciprocal interactions between human mesenchymal stem cells and gammadelta T cells or invariant natural killer T cells.
TCR2720 was used in ELISA to investigate the interactions between mesenchymal stem cells and gammadelta T cells
|Prigione I,Benvenuto F,Bocca P,Battistini L,Uccelli A,Pistoia V||Stem cells (Dayton, Ohio) (27:693)||2009|