|Tested species reactivity||Mouse|
|Published species reactivity||Mouse|
|Host / Isotype||Armenian hamster / IgG|
|Immunogen||TCR affinity purified from mouse T cell hybridoma DO-11.101|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||0.2 µg/10^6 cells|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 10 publications below|
The ability of T cell receptors (TCR) to discriminate foreign from self-peptides presented by major histocompatibility complex (MHC) class II molecules is essential for an effective adaptive immune response. TCR recognition of self-peptides has been linked to autoimmune disease. Mutant self-peptides have been associated with tumors. Engagement of TCRs by a family of bacterial toxins know as superantigens has been responsible for toxic shock syndrome. Autoantibodies to V beta segments of T cell receptors have been isolated from patients with rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE). The autoantibodies block TH1-mediated inflammatory autodestructive reactions and are believed to be a method by which the immune system compensates for disease (ref5). T Cell and TCR Diversity Most human T cells express the TCR alpha-beta and either CD4 or CD8 molecule (single positive, SP). A small number of T cells lack both CD4 and CD8 (double negative, DN). Increased percentages of alpha-beta DN T cells have been identified in some autoimmune and immunodeficiency disorders. Gamma-delta T cells are primarily found within the epithelium. They show less TCR diversity and recognize antigens differently than alpha-beta T cells. Subsets of gamma-delta T cells have shown antitumor and immunoregulatory activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Maternal immune stimulation during pregnancy affects adaptive immunity in offspring to promote development of TH17 cells.
A15408 was used in flow cytometry to examine how maternal immune stimulation affects the adaptive immune system and pathobiology of the offspring
|Mandal M,Marzouk AC,Donnelly R,Ponzio NM||Brain, behavior, and immunity (25:863)||2011|
Optimal colocalization of TCR and CD8 as a novel mechanism for the control of functional avidity.
A15408 was used in flow cytometry to investigate the control of functional avidity in response to viral infection.
|Cawthon AG,Alexander-Miller MA||Journal of immunology (Baltimore, Md. : 1950) (169:3492)||2002|
Diminution of the AML1 transcription factor function causes differential effects on the fates of CD4 and CD8 single-positive T cells.
A15408 was used in flow cytometry to investigate the role of AML1 in T cell development.
|Hayashi K,Natsume W,Watanabe T,Abe N,Iwai N,Okada H,Ito Y,Asano M,Iwakura Y,Habu S,Takahama Y,Satake M||Journal of immunology (Baltimore, Md. : 1950) (165:6816)||2000|
Identification of an early T cell progenitor for a pathway of T cell maturation in the bone marrow.
A15408 was used in flow cytometry to identify a rare population of cells in the adult mouse bone marrow that generates CD4(+) and CD8(+) TCRalphabeta(+) T cells.
|Dejbakhsh-Jones S,Strober S||Proceedings of the National Academy of Sciences of the United States of America (96:14493)||1999|
Commitment of common T/Natural killer (NK) progenitors to unipotent T and NK progenitors in the murine fetal thymus revealed by a single progenitor assay.
A15408 was used in flow cytometry to describe a clonal system that supports the development of T and natural killer cells evenly.
|Ikawa T,Kawamoto H,Fujimoto S,Katsura Y||The Journal of experimental medicine (190:1617)||1999|
Emergence of T, B, and myeloid lineage-committed as well as multipotent hemopoietic progenitors in the aorta-gonad-mesonephros region of day 10 fetuses of the mouse.
A15408 was used in flow cytometry to investigate the aorta-gonad-mesonephros region as a source of hemopoietic progenitors.
|Ohmura K,Kawamoto H,Fujimoto S,Ozaki S,Nakao K,Katsura Y||Journal of immunology (Baltimore, Md. : 1950) (163:4788)||1999|
Emergence of T cell progenitors without B cell or myeloid differentiation potential at the earliest stage of hematopoiesis in the murine fetal liver.
A15408 was used in flow cytometry to assess if progenitors colonizing the thymus are T cell lineage restricted.
|Kawamoto H,Ohmura K,Fujimoto S,Katsura Y||Journal of immunology (Baltimore, Md. : 1950) (162:2725)||1999|
Exclusive development of T cell neoplasms in mice transplanted with bone marrow expressing activated Notch alleles.
A15408 was used in flow cytometry to report that TAN1 is an oncoprotein.
|Pear WS,Aster JC,Scott ML,Hasserjian RP,Soffer B,Sklar J,Baltimore D||The Journal of experimental medicine (183:2283)||1996|
Reduced development of CD4-8-B220+ T cells but normal autoantibody production in lpr/lpr mice lacking major histocompatibility complex class I molecules.
A15408 was used in flow cytometry to examine the origin of double negative T cells using Ipr mice.
|Ohteki T,Iwamoto M,Izui S,MacDonald HR||European journal of immunology (25:37)||1995|
Major histocompatibility complex class I related molecules control the development of CD4+8- and CD4-8- subsets of natural killer 1.1+ T cell receptor-alpha/beta+ cells in the liver of mice.
A15408 was used in flow cytometry to investigate T cell subsets in the liver.
|Ohteki T,MacDonald HR||The Journal of experimental medicine (180:699)||1994|