|Tested species reactivity||Mouse|
|Published species reactivity||Not Applicable|
|Host / Isotype||Armenian hamster / IgG|
|Immunogen||Affinity Purified D0-11.10 TCR|
|Storage buffer||PBS with 4-5mg/ml BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1.0 ug/10^6 cells|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Flow Cytometry (Flow)||See 1 publications below|
MA5-17649 targets TCR alpha/beta in FACS and IHC (F) applications and shows reactivity with Mouse samples.
The MA5-17649 immunogen is affinity Purified D0-11.10 TCR.
The T cell antigen receptor (TCR) consists of a ligand-specific alpha/beta heterodimer non-covalently associated with five invariant chains including the CD3 gamma/delta/eta and zeta subunits, all of which are required for efficient surface expression. T cell activation through the TCR induces cellular differentiation and/or proliferation and the production of lymphokines and cytokines. Both the CD3 and TCR zeta subunits are proposed to be responsible for the intracellular signal transduction events.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Frequency of natural regulatory CD4+CD25+ T lymphocytes determines the outcome of tolerance across fully mismatched MHC barrier through linked recognition of self and allogeneic stimuli.
MA5-17649 was used in flow cytometry to discuss factors that regulate the long-term tolerance of allogeneic skin grafts in the absence of immunosuppression
|Fucs R,Jesus JT,Souza Junior PH,Franco L,Verícimo M,Bellio M,Nobrega A||Journal of immunology (Baltimore, Md. : 1950) (176:2324)||2006|