|Tested species reactivity||Mouse|
|Published species reactivity||Mouse, Not Applicable|
|Host / Isotype||Armenian hamster / IgG|
|Immunogen||Affinity-purified DO-11.10 TCR|
|Storage buffer||PBS with 4-5mg/ml BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||0.2 ug/10^6 cells|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-17652 targets TCR alpha/beta in FACS applications and shows reactivity with Mouse samples.
The MA5-17652 immunogen is affinity-purified DO-11.10 TCR.
The T cell antigen receptor (TCR) consists of a ligand-specific alpha/beta heterodimer non-covalently associated with five invariant chains including the CD3 gamma/delta/eta and zeta subunits, all of which are required for efficient surface expression. T cell activation through the TCR induces cellular differentiation and/or proliferation and the production of lymphokines and cytokines. Both the CD3 and TCR zeta subunits are proposed to be responsible for the intracellular signal transduction events.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Thymic alterations in mice deficient for the SNARE protein VAMP8/endobrevin.
MA5-17652 was used in flow cytometry to elucidate the role of VAMP8 in the thymus
|Kanwar N,Fayyazi A,Backofen B,Nitsche M,Dressel R,von Mollard GF||Cell and tissue research (334:227)||2008|
|Not Applicable||Not Cited||
Transgenic TCR expression: comparison of single chain with full-length receptor constructs for T-cell function.
MA5-17652 was used in flow cytometry to investigate the use of three-domain single-chain T-cell receptors
|Zhang T,He X,Tsang TC,Harris DT||Cancer gene therapy (11:487)||2004|
Involvement of p38 mitogen-activated protein kinase in different stages of thymocyte development.
MA5-17652 was used in flow cytometry to suggest that p38 is involved in positive selection of T cells.
|Hsu SC,Wu CC,Han J,Lai MZ||Blood (101:970)||2003|
Candida albicans infection enhances immunosuppression induced by cyclophosphamide by selective priming of suppressive myeloid progenitors for NO production.
MA5-17652 was used in flow cytometry to examine the T cell responses in cyclophosphamide-treated mice after systemic infection with Candida albicans.
|Angulo I,Jiménez-Díaz MB,García-Bustos JF,Gargallo D,de las Heras FG,Muñoz-Fernández MA,Fresno M||Cellular immunology (218:46)||2002|
Increased p300 expression inhibits glucocorticoid receptor-T-cell receptor antagonism but does not affect thymocyte positive selection.
MA5-17652 was used in flow cytometry to test if T-cell-specific expression of p300 alters GR-TCR cross talk between thymocytes.
|Yu CT,Feng MH,Shih HM,Lai MZ||Molecular and cellular biology (22:4556)||2002|
Differential in vivo persistence of two subsets of memory phenotype CD8 T cells defined by CD44 and CD122 expression levels.
MA5-17652 was used in flow cytometry to characterize two subsets of mouse CD8 T cells.
|Walzer T,Arpin C,Beloeil L,Marvel J||Journal of immunology (Baltimore, Md. : 1950) (168:2704)||2002|
IL-18 enhances IL-4 production by ligand-activated NKT lymphocytes: a pro-Th2 effect of IL-18 exerted through NKT cells.
MA5-17652 was used in flow cytometry to investigate the effect of IL-18 on NKT cells.
|Leite-De-Moraes MC,Hameg A,Pacilio M,Koezuka Y,Taniguchi M,Van Kaer L,Schneider E,Dy M,Herbelin A||Journal of immunology (Baltimore, Md. : 1950) (166:945)||2001|
Downmodulation of the inflammatory response to bacterial infection by gammadelta T cells cytotoxic for activated macrophages.
MA5-17652 was used in flow cytometry to propose that gammadelta T cells eliminating activated macrophages after infection with an intracellular pathogen.
|Egan PJ,Carding SR||The Journal of experimental medicine (191:2145)||2000|
Nitric oxide-producing CD11b(+)Ly-6G(Gr-1)(+)CD31(ER-MP12)(+) cells in the spleen of cyclophosphamide-treated mice: implications for T-cell responses in immunosuppressed mice.
MA5-17652 was used in flow cytometry to study T-cell unresponsiveness in cyclophosphamide treated mice.
|Angulo I,de las Heras FG,García-Bustos JF,Gargallo D,Muñoz-Fernández MA,Fresno M||Blood (95:212)||2000|
Identification of an early T cell progenitor for a pathway of T cell maturation in the bone marrow.
MA5-17652 was used in flow cytometry to identify a rare population of cells in the adult mouse bone marrow that generates CD4(+) and CD8(+) TCRalphabeta(+) T cells.
|Dejbakhsh-Jones S,Strober S||Proceedings of the National Academy of Sciences of the United States of America (96:14493)||1999|