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Flow cytometry analysis of TCR gamma/delta on TCR gamma/delta positive PEER cells or TCR gamma/delta negative Jurkat cells. Equal numbers of cells were stained with a TCR gamma + delta (5A6.E9) monoclonal antibody (Product # TCR1061) followed by FITC labeled secondary antibody or with FITC labeled secondary antibody alone (black histograms). 5ul of primary antibody were used per test. All antibody incubations were performed for 30 minutes at room temperature. A representative 10,000 cells were acquired for each sample.
|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Human leukemic PEER cells|
|Storage buffer||PBS with 0.5% BSA|
|Contains||0.1% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1-2 µg/test|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|T-Cell Activation (TCA)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
TCR1061 targets TCR Pan TCR gamma delta in FACS, IHC(F), IP, and TCA applications and shows reactivity with Human samples. The clone 5A6.E9 has been listed as 5A6.E91 in some publications and documents. These are the same clone.
The TCR1061 immunogen is human PEER cells.
TCR1061 detects TCR Pan TCR gamma delta which has a predicted molecular weight of approximately 18 kDa.
The ability of T cell receptors (TCR) to discriminate foreign from self-peptides presented by major histocompatibility complex (MHC) class II molecules is essential for an effective adaptive immune response. TCR recognition of self-peptides has been linked to autoimmune disease. Mutant self-peptides have been associated with tumors. Engagement of TCRs by a family of bacterial toxins know as superantigens has been responsible for toxic shock syndrome. Autoantibodies to V beta segments of T cell receptors have been isolated from patients with rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE). The autoantibodies block TH1-mediated inflammatory autodestructive reactions and are believed to be a method by which the immune system compensates for disease (ref5). T Cell and TCR Diversity Most human T cells express the TCR alpha-beta and either CD4 or CD8 molecule (single positive, SP). A small number of T cells lack both CD4 and CD8 (double negative, DN). Increased percentages of alpha-beta DN T cells have been identified in some autoimmune and immunodeficiency disorders. Gamma-delta T cells are primarily found within the epithelium. They show less TCR diversity and recognize antigens differently than alpha-beta T cells. Subsets of gamma-delta T cells have shown antitumor and immunoregulatory activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Distinct morphologic, phenotypic, and clinical-course characteristics of indolent peripheral T-cell lymphoma.
TCR1061 was used in immunohistochemistry to report on the clinicopathology of 10 cases of peripheral T-cell lymphoma
|Hayashi E,Takata K,Sato Y,Tashiro Y,Tachiyama Y,Sawada-Kitamura S,Hiramatsu Y,Sugiguchi S,Nose S,Hirokawa M,Ando M,Abd Alkader L,Maeda Y,Tanimoto M,Yoshino T||Human pathology (44:1927)||2013|
Nonhepatosplenic γδ T-cell lymphomas represent a spectrum of aggressive cytotoxic T-cell lymphomas with a mainly extranodal presentation.
TCR1061 was used in immunohistochemistry to investigate the clinical heterogeneity of nonhepatosplenic gamma-delta T-cell lymphomas
|Garcia-Herrera A,Song JY,Chuang SS,Villamor N,Colomo L,Pittaluga S,Alvaro T,Rozman M,de Anda Gonzalez J,Arrunategui AM,Fernandez E,Gonzalvo E,Estrach T,Colomer D,Raffeld M,Gaulard P,Campo E,Jaffe ES,Martinez A||The American journal of surgical pathology (35:1214)||2011|
Maternal Foxp3 expressing CD4+ CD25+ and CD4+ CD25- regulatory T-cell populations are enriched in human early normal pregnancy decidua: a phenotypic study of paired decidual and peripheral blood samples.
TCR1061 was used in immunohistochemistry to identify relationship between T-cell populations and pregnancy
|Dimova T,Nagaeva O,Stenqvist AC,Hedlund M,Kjellberg L,Strand M,Dehlin E,Mincheva-Nilsson L||American journal of reproductive immunology (New York, N.Y. : 1989) (66 Suppl 1:44)||2011|
IL-23/IL-17 immunity as a hallmark of Crohn's disease.
TCR1061 was used in immunohistochemistry to study IL-23/IL-17 immunity as a hallmark of Crohn's disease
|Hölttä V,Klemetti P,Sipponen T,Westerholm-Ormio M,Kociubinski G,Salo H,Räsänen L,Kolho KL,Färkkilä M,Savilahti E,Vaarala O||Inflammatory bowel diseases (14:1175)||2008|
Cytotoxic T cells are preferentially activated in the duodenal epithelium from patients with florid coeliac disease.
TCR1061 was used in immunohistochemistry to investigate the activation of cytotoxic T cells in duodenal epithelium from patients with florid coeliac disease
|Buri C,Burri P,Bähler P,Straumann A,Müller-Schenker B,Birrer S,Mueller C||The Journal of pathology (206:178)||2005|
Clinicopathologic analysis of 22 cases of subcutaneous panniculitis-like CD56- or CD56+ lymphoma and review of 44 other reported cases.
TCR1061 was used in immunohistochemistry to study subcutaneous panniculitis-like CD56- or CD56+ lymphoma during labor
|Takeshita M,Imayama S,Oshiro Y,Kurihara K,Okamoto S,Matsuki Y,Nakashima Y,Okamura T,Shiratsuchi M,Hayashi T,Kikuchi M||American journal of clinical pathology (121:408)||2004|
Flexible migration program regulates gamma delta T-cell involvement in humoral immunity.
TCR1061 was used in immunohistochemistry to investigate the migration of gamma delta T cells
|Brandes M,Willimann K,Lang AB,Nam KH,Jin C,Brenner MB,Morita CT,Moser B||Blood (102:3693)||2003|
Inflammatory cytokines in small intestinal mucosa of patients with potential coeliac disease.
TCR1061 was used in immunohistochemistry to investigate the expression of inflammatory cytokines in small intestinal mucosa of patients with potential coeliac disease
|Westerholm-Ormio M,Garioch J,Ketola I,Savilahti E||Clinical and experimental immunology (128:94)||2002|
BCL-6 protein is expressed in precursor T-cell lymphoblastic lymphoma and in prenatal and postnatal thymus.
TCR1061 was used in immunohistochemistry to study the expression of BCL-6 protein in precursor T-cell lymphoblastic lymphoma and in prenatal and postnatal thymus
|Hyjek E,Chadburn A,Liu YF,Cesarman E,Knowles DM||Blood (97:270)||2001|
Unaltered distribution of laminins, fibronectin, and tenascin in celiac intestinal mucosa.
TCR1061 was used in immunohistochemistry to investigate the expression and distribution of laminins, fibronectin, and tenascin in celiac intestinal mucosa
|Korhonen M,Ormio M,Burgeson RE,Virtanen I,Savilahti E||The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (48:1011)||2000|
Small bowel T cells, HLA class II antigen DR, and GroEL stress protein in IgA nephropathy.
TCR1061 was used in immunohistochemistry to study the roles of intestinal mucosa in the pathogenesis of IgA nephropathy
|Rantala I,Collin P,Holm K,Kainulainen H,Mustonen J,Mäki M||Kidney international (55:2274)||1999|
Human mucosal associated invariant T cells detect bacterially infected cells.
TCR1061 was used in blocking or activating experiment to investigate the role of nonclassically restricted CD8+ T cells during Mycobacterium tuberculosis infection
|Gold MC,Cerri S,Smyk-Pearson S,Cansler ME,Vogt TM,Delepine J,Winata E,Swarbrick GM,Chua WJ,Yu YY,Lantz O,Cook MS,Null MD,Jacoby DB,Harriff MJ,Lewinsohn DA,Hansen TH,Lewinsohn DM||PLoS biology (8:null)||2010|
Autosomal dominant and sporadic monocytopenia with susceptibility to mycobacteria, fungi, papillomaviruses, and myelodysplasia.
TCR1061 was used in flow cytometry to study the clinical relation between autosomal dominant and sporadic monocytopenia and susceptibility to mycobacteria, fungi, papillomaviruses, and myelodysplasia
|Vinh DC,Patel SY,Uzel G,Anderson VL,Freeman AF,Olivier KN,Spalding C,Hughes S,Pittaluga S,Raffeld M,Sorbara LR,Elloumi HZ,Kuhns DB,Turner ML,Cowen EW,Fink D,Long-Priel D,Hsu AP,Ding L,Paulson ML,Whitney AR,Sampaio EP,Frucht DM,DeLeo FR,Holland SM||Blood (115:1519)||2010|
Human placenta expresses and secretes NKG2D ligands via exosomes that down-modulate the cognate receptor expression: evidence for immunosuppressive function.
TCR1061 was used in flow cytometry to investigate the role of NKG2D ligand-bearing placental exosomes in human placenta
|Hedlund M,Stenqvist AC,Nagaeva O,Kjellberg L,Wulff M,Baranov V,Mincheva-Nilsson L||Journal of immunology (Baltimore, Md. : 1950) (183:340)||2009|
Human CD1-restricted T cell recognition of lipids from pollens.
TCR1061 was used in flow cytometry to study the immune response of pollen lipids
|Agea E,Russano A,Bistoni O,Mannucci R,Nicoletti I,Corazzi L,Postle AD,De Libero G,Porcelli SA,Spinozzi F||The Journal of experimental medicine (202:295)||2005|
Recognition of nonpeptide antigens by human V gamma 9V delta 2 T cells requires contact with cells of human origin.
TCR1061 was used in flow cytometry to study the mechanism for the immune response against nonpeptide antigens by human V gamma 9V delta 2 T cells
|Green AE,Lissina A,Hutchinson SL,Hewitt RE,Temple B,James D,Boulter JM,Price DA,Sewell AK||Clinical and experimental immunology (136:472)||2004|
Ontogenic changes in CD95 expression on human leukocytes: prevalence of T-cells expressing activation markers and identification of CD95-CD45RO+ T-cells in the fetus.
TCR1061 was used in flow cytometry to study the high level expression of T-cell activation markers in the fetus
|Muench MO,Pott Bärtsch EM,Chen JC,Lopoo JB,Bárcena A||Developmental and comparative immunology (27:899)||2003|
Human double-negative T cells in systemic lupus erythematosus provide help for IgG and are restricted by CD1c.
TCR1061 was used in flow cytometry to study the role of CD4- and CD8- double negative T cells in providing help for IgG production in systemic lupus erythematosus.
|Sieling PA,Porcelli SA,Duong BT,Spada F,Bloom BR,Diamond B,Hahn BH||Journal of immunology (Baltimore, Md. : 1950) (165:5338)||2000|
New monoclonal antibody (23D12) recognizing three different V gamma elements of the human gamma delta T cell receptor. 23D12+ cells comprise a major subpopulation of gamma delta T cells in postnatal thymus.
TCR1061 was used in flow cytometry to characterize a monoclonal antibody against human gamma delta T cell receptor
|Kabelitz D,Ackermann T,Hinz T,Davodeau F,Band H,Bonneville M,Janssen O,Arden B,Schondelmaier S||Journal of immunology (Baltimore, Md. : 1950) (152:3128)||1994|
Human cytotoxic lymphocytes. V. Frequency and specificity of gamma delta+ cytotoxic lymphocyte precursors activated by allogeneic or autologous stimulator cells.
TCR1061 was used in flow cytometry to characterize gamma delta+ cytotoxic lymphocyte precursors and its activation mechanism
|Kabelitz D,Bender A,Schondelmaier S,da Silva Lobo ML,Janssen O||Journal of immunology (Baltimore, Md. : 1950) (145:2827)||1990|
Demographic and immune correlates of human herpesvirus 8 seropositivity in Malawi, Africa.
TCR1061 was used in ELISA to investigate the status of human herpesvirus 8 seropositivity in Malawians
|DeSantis SM,Pau CP,Archibald LK,Nwanyanwu OC,Kazembe PN,Dobbie H,Jarvis WR,Jason J||International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases (6:266)||2002|
Lyme arthritis synovial gamma delta T cells respond to Borrelia burgdorferi lipoproteins and lipidated hexapeptides.
TCR1061 was used in flow cytometry to study synovial V delta 1 T cells during infection with B. burgdorferi.
|Vincent MS,Roessner K,Sellati T,Huston CD,Sigal LH,Behar SM,Radolf JD,Budd RC||Journal of immunology (Baltimore, Md. : 1950) (161:5762)||1998|