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Flow cytometry analysis of TCR gamma/delta on TCR gamma/delta positive PEER cells or TCR gamma/delta negative Jurkat cells. Equal numbers of cells were stained with a FITC-labeled TCR gamma + delta (5A6.E9) monoclonal antibody (Product # TCR2061) or were left unstained (black histograms). 5ul of primary antibody were used per test. All antibody incubations were performed for 30 minutes at room temperature. A representative 10,000 cells were acquired for each sample.
|Tested species reactivity||Human|
|Published species reactivity||Primate, Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Pan TCR gamma delta|
|Storage buffer||PBS with 0.5% BSA, glycerol|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1-2 µg/test|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
TCR2061 targets TCR Pan TCR gamma delta in FACS applications and shows reactivity with Human samples.
The TCR2061 immunogen is pan TCR gamma delta.
TCR2061 detects TCR Pan TCR gamma delta which has a predicted molecular weight of approximately 18 kDa.
The ability of T cell receptors (TCR) to discriminate foreign from self-peptides presented by major histocompatibility complex (MHC) class II molecules is essential for an effective adaptive immune response. TCR recognition of self-peptides has been linked to autoimmune disease. Mutant self-peptides have been associated with tumors. Engagement of TCRs by a family of bacterial toxins know as superantigens has been responsible for toxic shock syndrome. Autoantibodies to V beta segments of T cell receptors have been isolated from patients with rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE). The autoantibodies block TH1-mediated inflammatory autodestructive reactions and are believed to be a method by which the immune system compensates for disease (ref5). T Cell and TCR Diversity Most human T cells express the TCR alpha-beta and either CD4 or CD8 molecule (single positive, SP). A small number of T cells lack both CD4 and CD8 (double negative, DN). Increased percentages of alpha-beta DN T cells have been identified in some autoimmune and immunodeficiency disorders. Gamma-delta T cells are primarily found within the epithelium. They show less TCR diversity and recognize antigens differently than alpha-beta T cells. Subsets of gamma-delta T cells have shown antitumor and immunoregulatory activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Nonhepatosplenic γδ T-cell lymphomas represent a spectrum of aggressive cytotoxic T-cell lymphomas with a mainly extranodal presentation.
TCR2061 was used in immunohistochemistry to investigate the clinical heterogeneity of nonhepatosplenic gamma-delta T-cell lymphomas
|Garcia-Herrera A,Song JY,Chuang SS,Villamor N,Colomo L,Pittaluga S,Alvaro T,Rozman M,de Anda Gonzalez J,Arrunategui AM,Fernandez E,Gonzalvo E,Estrach T,Colomer D,Raffeld M,Gaulard P,Campo E,Jaffe ES,Martinez A||The American journal of surgical pathology (35:1214)||2011|
IL-23/IL-17 immunity as a hallmark of Crohn's disease.
TCR2061 was used in immunohistochemistry to study IL-23/IL-17 immunity as a hallmark of Crohn's disease
|Hölttä V,Klemetti P,Sipponen T,Westerholm-Ormio M,Kociubinski G,Salo H,Räsänen L,Kolho KL,Färkkilä M,Savilahti E,Vaarala O||Inflammatory bowel diseases (14:1175)||2008|
Clinicopathologic analysis of 22 cases of subcutaneous panniculitis-like CD56- or CD56+ lymphoma and review of 44 other reported cases.
TCR2061 was used in immunohistochemistry to study subcutaneous panniculitis-like CD56- or CD56+ lymphoma during labor
|Takeshita M,Imayama S,Oshiro Y,Kurihara K,Okamoto S,Matsuki Y,Nakashima Y,Okamura T,Shiratsuchi M,Hayashi T,Kikuchi M||American journal of clinical pathology (121:408)||2004|
Flexible migration program regulates gamma delta T-cell involvement in humoral immunity.
TCR2061 was used in immunohistochemistry to investigate the migration of gamma delta T cells
|Brandes M,Willimann K,Lang AB,Nam KH,Jin C,Brenner MB,Morita CT,Moser B||Blood (102:3693)||2003|
Unaltered distribution of laminins, fibronectin, and tenascin in celiac intestinal mucosa.
TCR2061 was used in immunohistochemistry to investigate the expression and distribution of laminins, fibronectin, and tenascin in celiac intestinal mucosa
|Korhonen M,Ormio M,Burgeson RE,Virtanen I,Savilahti E||The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (48:1011)||2000|
Small bowel T cells, HLA class II antigen DR, and GroEL stress protein in IgA nephropathy.
TCR2061 was used in immunohistochemistry to study the roles of intestinal mucosa in the pathogenesis of IgA nephropathy
|Rantala I,Collin P,Holm K,Kainulainen H,Mustonen J,Mäki M||Kidney international (55:2274)||1999|
Restricted usage of T cell receptor V alpha-V beta genes in infiltrating cells in the hearts of patients with acute myocarditis and dilated cardiomyopathy.
TCR2061 was used in immunohistochemistry to study the mechanism for the pathogenesis of dilated cardiomyopathy
|Seko Y,Ishiyama S,Nishikawa T,Kasajima T,Hiroe M,Kagawa N,Osada K,Suzuki S,Yagita H,Okumura K||The Journal of clinical investigation (96:1035)||1995|
Human mucosal associated invariant T cells detect bacterially infected cells.
TCR2061 was used in blocking or activating experiment to investigate the role of nonclassically restricted CD8+ T cells during Mycobacterium tuberculosis infection
|Gold MC,Cerri S,Smyk-Pearson S,Cansler ME,Vogt TM,Delepine J,Winata E,Swarbrick GM,Chua WJ,Yu YY,Lantz O,Cook MS,Null MD,Jacoby DB,Harriff MJ,Lewinsohn DA,Hansen TH,Lewinsohn DM||PLoS biology (8:null)||2010|
Human placenta expresses and secretes NKG2D ligands via exosomes that down-modulate the cognate receptor expression: evidence for immunosuppressive function.
TCR2061 was used in flow cytometry to investigate the role of NKG2D ligand-bearing placental exosomes in human placenta
|Hedlund M,Stenqvist AC,Nagaeva O,Kjellberg L,Wulff M,Baranov V,Mincheva-Nilsson L||Journal of immunology (Baltimore, Md. : 1950) (183:340)||2009|
Human CD1-restricted T cell recognition of lipids from pollens.
TCR2061 was used in flow cytometry to study the immune response of pollen lipids
|Agea E,Russano A,Bistoni O,Mannucci R,Nicoletti I,Corazzi L,Postle AD,De Libero G,Porcelli SA,Spinozzi F||The Journal of experimental medicine (202:295)||2005|
Are gamma delta T cells important for the elimination of virus-infected cells?
TCR2061 was used in flow cytometry to investigate the role of gamma delta T cells in the killing of virus-infected cells
|Malkovsky M,Bartz SR,MacKenzie D,Radtke BE,Wallace M,Manning J,Pauza CD,Fisch P||Journal of medical primatology (21:113)||1992|
Demographic and immune correlates of human herpesvirus 8 seropositivity in Malawi, Africa.
TCR2061 was used in ELISA to investigate the status of human herpesvirus 8 seropositivity in Malawians
|DeSantis SM,Pau CP,Archibald LK,Nwanyanwu OC,Kazembe PN,Dobbie H,Jarvis WR,Jason J||International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases (6:266)||2002|
Intraepithelial T cells of the TcR gamma/delta+ CD8- and V delta 1/J delta 1+ phenotypes are increased in coeliac disease.
TCR2061 was used in immunocytochemistry to investigate the changes of TCR gamma/delta+ CD8- T cells in patients with coeliac disease
|Halstensen TS,Scott H,Brandtzaeg P||Scandinavian journal of immunology (30:665)||1989|