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OriGene
TDG Monoclonal Antibody (OTI3C11), TrueMAB™
Advanced Verification
This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.
Product Details
CF808610
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
OTI3C11
Immunogen
Human recombinant protein fragment corresponding to amino acids 160-253 of human TDG produced in E.coli.
Conjugate
Form
Lyophilized
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 8% trehalose
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
Target Information
The protein encoded by this gene belongs to the TDG/mug DNA glycosylase family. Thymine-DNA glycosylase removes thymine moieties from G/T mismatches by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of DNA and the mis paired thymine. With lower activity, this enzyme also removes thymine from C/T and T/T mispairings. TDG can also remove uracil and 5-bromouracil from mispairings with guanine. This enzyme plays a central role in cellular defense against genetic mutation caused by the spontaneous deamination of 5-methylcytosine and cytosine. This gene may have a pseudogene in the p arm of chromosome 12.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: C-JUN leucine zipper interactive protein JZA-3; G/T mismatch-specific thymine DNA glycosylase; hTDG; Thymine-DNA glycosylase
Gene Aliases: E130317C12Rik; hTDG; JZA-3; Jza1; TDG
UniProt ID: (Human) Q13569, (Mouse) P56581
Entrez Gene ID: (Human) 6996, (Rat) 114521, (Mouse) 21665
RNA polymerase II transcription cofactor activity
damaged DNA binding
double-stranded DNA binding
uracil DNA N-glycosylase activity
protein kinase C binding
protein binding
pyrimidine-specific mismatch base pair DNA N-glycosylase activity
DNA N-glycosylase activity
mismatched DNA binding
SUMO binding
protein homodimerization activity
structure-specific DNA binding
transcription factor binding
hydrolase activity, hydrolyzing N-glycosyl compounds
protein domain specific binding
hydrolase activity
hydrolase activity, acting on glycosyl bonds
DNA metabolism protein
DNA glycosylase
nucleic acid metabolism protein
negative regulation of transcription from RNA polymerase II promoter
base-excision repair
base-excision repair, AP site formation
mismatch repair
transcription, DNA-templated
embryo development
covalent chromatin modification
protein sumoylation
negative regulation of protein binding
oxidative DNA demethylation
negative regulation of chromatin binding
regulation of gene expression, epigenetic
depyrimidination
DNA demethylation
transcription from RNA polymerase II promoter
DNA repair
regulation of transcription, DNA-templated
cellular response to DNA damage stimulus
metabolic process
chromatin modification
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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