OriGene

TDG Monoclonal Antibody (OTI3C11), TrueMAB™

Advanced Verification

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated. View Details

View all (23) TDG antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

TDG Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical staining of paraffin-embedded human lymph node tissue within the normal limits using anti-TDG mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808610)(1:150).

TDG Antibody in Western Blot (WB) — HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TDG (RC207113, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-TDG. (1:2. Positive lysates LY401109 (100 µg) and LC401109 (20 µg) can be purchased separately from OriGene.

TDG Antibody — Antibody specificity was demonstrated by CRISPR-Cas9 mediated knockout of target protein. Loss of signal was observed for target protein in TDG knockout (KO) Hela cell line using Anti-TDG monoclonal antibody (Product # TA808610). {KO}

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000

Immunohistochemistry (Paraffin) (IHC (P))

1:150

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

OTI3C11

Immunogen

Human recombinant protein fragment corresponding to amino acids 160-253 of human TDG produced in E.coli.

Conjugate

Unconjugated

Form

liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS with 1% BSA, 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Target Information

The protein encoded by this gene belongs to the TDG/mug DNA glycosylase family. Thymine-DNA glycosylase removes thymine moieties from G/T mismatches by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of DNA and the mis paired thymine. With lower activity, this enzyme also removes thymine from C/T and T/T mispairings. TDG can also remove uracil and 5-bromouracil from mispairings with guanine. This enzyme plays a central role in cellular defense against genetic mutation caused by the spontaneous deamination of 5-methylcytosine and cytosine. This gene may have a pseudogene in the p arm of chromosome 12.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.