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Immunoprecipitation analysis using Anti-TDR2 Recombinant Mouse Monoclonal Antibody (Cat. no. 730053) followed by western blot detection. Immunoprecipitation was performed on HEK293 cells over expressing flag-tagged TDR2 protein. Western blot using an anti-flag antibody was performed on the raw lysate (lane 1) and immunoprecipitated protein (lane 2) to evaluate the results of Immunoprecipitation. Because detection utilizes an anti-flag antibody, the presence of multiple bands suggests multiple forms of flag-tagged TDR2 protein rather than cross-reactivity of the Anti-TDR2 Recombinant Mouse Monoclonal Antibody.
|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||48-561 of 561 of TDRKH Protein.|
|Contains||0.09% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Immunoprecipitation (IP)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunoprecipitation (IP)||See 1 publications below|
TDRKH is a protein of unknown function containing one tudor domain, and two KH domains. It has been found to interact with PIWIL1 and PIWIL4 by binding to methylated arginines residues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Assessment of a method to characterize antibody selectivity and specificity for use in immunoprecipitation.
730053 was used in immunoprecipitation to describe a mass spectrometry-based method for scoring immunoprecipitation antibody quality
|Marcon E,Jain H,Bhattacharya A,Guo H,Phanse S,Pu S,Byram G,Collins BC,Dowdell E,Fenner M,Guo X,Hutchinson A,Kennedy JJ,Krastins B,Larsen B,Lin ZY,Lopez MF,Loppnau P,Miersch S,Nguyen T,Olsen JB,Paduch M,Ravichandran M,Seitova A,Vadali G,Vogelsang MS,Whiteaker JR,Zhong G,Zhong N,Zhao L,Aebersold R,Arrowsmith CH,Emili A,Frappier L,Gingras AC,Gstaiger M,Paulovich AG,Koide S,Kossiakoff AA,Sidhu SS,Wodak SJ,Gr?slund S,Greenblatt JF,Edwards AM||Nature methods (12:725)||2015|
putative RNA binding protein, tudor and KH domain containing protein, tudor and KH domain-containing protein, tudor domain containing 2, tudor domain-containing protein 2, TDRD2