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|Tested species reactivity||Human, Mouse|
|Host / Isotype||Mouse / IgM|
|Immunogen||Full-length recombinant hTERT from insect cells.|
|Contains||0.02% sodium azide|
|Storage Conditions||-20°C or -80°C if preferred|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:50-1:200|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50|
|Western Blot (WB)||1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Suggested positive control: 293T whole cell lysates, HeLa whole cell lysates, antigen standard for TERT (transient overexpression lysate).
Telomeres are nucleoprotein structures at the ends of eukaryotic chromosomes. They regulate several crucial cellular functions and participate in the control of complex phenotypes, such as aging and cancer. Cell immortalization is strongly associated with the stabilization of telomere length. Therefore, it is suggested that cancer cells achieve immortalization in part through illegitimate activation of telomerase expression. hTERT (TERT) is the rate-limiting catalytic subunit of telomerase.
Telomerase is an enzyme uniquely associated with immortal cell lines and is useful for identifying undifferentiated PSCs.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
DKCA2; DKCB4; EST2; HEST2; hTERT; hTRT; TCS1; Telomerase catalytic subunit; Telomerase reverse transcriptase; telomerase reverse transcriptase beta; Telomerase-associated protein 2; TP2; TRT
CMM9; DKCA2; DKCB4; EST2; hEST2; hTRT; PFBMFT1; TCS1; TERT; TP2; TR; TRT