Immunofluorescent analysis of TET1 showing staining in the nucleus of 293T cells. TET1-transfected (right) or untransfected (left) 293T cells were fixed in 4% paraformaldehyde for 15 min and stained using a TET1 monoclonal antibody (Product # MA5-16312) diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar = 10µm.
|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||tet oncogene 1|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ChIP assay (ChIP)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-16312 targets TET1 in WB, ICC, IP, ChIP, IHC(P) and IF applications and shows reactivity with Human samples.
The MA5-16312 immunogen is tet oncogene 1.
Dioxygenase that specifically binds methylcytosine (5mC), a minor base in mammalian DNA found in repetitive DNA elements that is crucial for retrotransposon silencing and mammalian development. Catalyzes the conversion of methylcytosine (5mC) to 5-hydroxymethylcytosine (hmC). The clear function of 5-hydroxymethylcytosine (hmC) is still unclear but it may influence chromatin structure and recruit specific factors or may constitute an intermediate component in cytosine demethylation. 5-hydroxymethylcytosine (hmC) is present in ES cells and is enriched in the brain, especially in Purkinje neurons. May play a role in the fetal development of heart, lung and brain.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.