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TET1 Recombinant Mouse Monoclonal Antibody, AbFlex®

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TET1 Antibody in Western Blot (WB) — Western blot of AbFlex® TET1 antibody. 20 µg nuclear lysate from F9 (mouse testis embryonal carcinoma) cells was probed by Western Blot using 2 µg/mL of antibody. MW: 215 kDa.

TET1 Antibody in ChIP-sequencing (ChIP-Seq) — AbFlex® TET1 antibody tested by ChIP-Seq. ChIP was performed using AbFlex TET1 recombinant antibody with 30 µg chromatin from human brain tumor cells and 4 µl of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 13 million sequence tags were mapped to identify TET1 binding sites. The image shows binding across a region of chromosome 1.

Product Details

91171

Applications	Tested Dilution	Publications
Western Blot (WB)	Assay-dependent	-
ChIP-sequencing (ChIP-Seq)	4 µL	-

Product Specifications
Species Reactivity	Human, Mouse
Host/Isotype	Mouse / IgG2a
Class	Recombinant Monoclonal
Type	Antibody
Immunogen	This antibody was raised against a recombinant protein comprising amino acids 1682-1914 of mouse TET1 (Ten-Eleven Translocation-1; methylcytosine dioxygenase). View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Protein A
Storage buffer	140mM HEPES/35mM sodium acetate, pH 7.5, with 30% glycerol, 70mM NaCl
Contains	0.035% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2793792

Target Information

Dioxygenase that specifically binds methylcytosine (5mC), a minor base in mammalian DNA found in repetitive DNA elements that is crucial for retrotransposon silencing and mammalian development. Catalyzes the conversion of methylcytosine (5mC) to 5-hydroxymethylcytosine (hmC). The clear function of 5-hydroxymethylcytosine (hmC) is still unclear but it may influence chromatin structure and recruit specific factors or may constitute an intermediate component in cytosine demethylation. 5-hydroxymethylcytosine (hmC) is present in ES cells and is enriched in the brain, especially in Purkinje neurons. May play a role in the fetal development of heart, lung and brain.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CXXC finger 6; CXXC zinc finger 6; CXXC-type zinc finger protein 6; Leukemia-associated protein with a CXXC domain; Methylcytosine dioxygenase TET1; RP11-119F7.1; Ten-eleven translocation 1 gene protein; ten-eleven translocation 1 gene protein homolog; ten-eleven translocation-1; tet oncogene 1; TET1 splice variant VP_DE4; TET1 splice variant VP_DE456

Gene Aliases: 2510010B09Rik; AA517754; bA119F7.1; BB001228; CXXC6; D10Ertd17e; KIAA1676; LCX; mKIAA1676; MLL-TET1; TET1; TET1-MLL

UniProt ID: (Human) Q8NFU7

Entrez Gene ID: (Human) 80312, (Mouse) 52463

Function(s)