Invitrogen

TET1 Monoclonal Antibody (GT1462)

Product Image Gallery

TET1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of TET1 showing staining in the nucleus of 293T cells. TET1-transfected (right) or untransfected (left) 293T cells were fixed in 4% paraformaldehyde for 15 min and stained using a TET1 monoclonal antibody (Product # MA5-16312) diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar = 10µm.

TET1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of TET1 in the nucleus of paraffin-embedded HeLa xenograft using a TET1 monoclonal antibody (Product # MA5-16312) at a dilution of 1:100.

TET1 Antibody in Western Blot (WB) — Western blot analysis of TET1 using A) 50 µg whole cell lysate from 293T cells transfected with scramble siRNA and B) 50 µg whole cell lysate from TET1-knockdowned 293T cells. Samples were loaded onto a 6% SDS-PAGE gel and probed with a TET1 monoclonal antibody (Product # MA5-16312) at a dilution of 1:500.

TET1 Antibody in Immunoprecipitation (IP) — Immunoprecipitation and Western blot of TET1 protein from NT2D1 whole cell extracts using 5 µg of TET1 antibody (Product # MA5-16312).

TET1 Antibody in ChIP assay (ChIP) — Published figure using TET1 monoclonal antibody (Product # MA5-16312) in ChIP assay

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Immunocytochemistry (ICC/IF)

1:100-1:1,000

Immunoprecipitation (IP)

1:100-1:500

ChIP assay (ChIP)

Assay-dependent

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Mouse

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

GT1462

Immunogen

tet oncogene 1

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2537831

Product Specific Information

Recommended positive controls: 293T, DDDDK-human TET1-transfected 293T, NT2D1, NT2D1 nuclear extract.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Dioxygenase that specifically binds methylcytosine (5mC), a minor base in mammalian DNA found in repetitive DNA elements that is crucial for retrotransposon silencing and mammalian development. Catalyzes the conversion of methylcytosine (5mC) to 5-hydroxymethylcytosine (hmC). The clear function of 5-hydroxymethylcytosine (hmC) is still unclear but it may influence chromatin structure and recruit specific factors or may constitute an intermediate component in cytosine demethylation. 5-hydroxymethylcytosine (hmC) is present in ES cells and is enriched in the brain, especially in Purkinje neurons. May play a role in the fetal development of heart, lung and brain.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: CXXC finger 6; CXXC zinc finger 6; CXXC-type zinc finger protein 6; Leukemia-associated protein with a CXXC domain; Methylcytosine dioxygenase TET1; RP11-119F7.1; Ten-eleven translocation 1 gene protein; ten-eleven translocation 1 gene protein homolog; ten-eleven translocation-1; tet oncogene 1; TET1 splice variant VP_DE4; TET1 splice variant VP_DE456

Gene Aliases: 2510010B09Rik; AA517754; bA119F7.1; BB001228; CXXC6; D10Ertd17e; KIAA1676; LCX; mKIAA1676; MLL-TET1; TET1; TET1-MLL

UniProt ID: (Human) Q8NFU7

Entrez Gene ID: (Human) 80312, (Mouse) 52463

Function(s)