Invitrogen

TET1 Monoclonal Antibody (GT1462)

Product Image Gallery

TET1 Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of TET1 showing staining in the nucleus of 293T cells. TET1-transfected (right) or untransfected (left) 293T cells were fixed in 4% paraformaldehyde for 15 min and stained using a TET1 monoclonal antibody (Product # MA5-16312) diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar = 10µm.

TET1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of TET1 in the nucleus of paraffin-embedded HeLa xenograft using a TET1 monoclonal antibody (Product # MA5-16312) at a dilution of 1:100.

TET1 Antibody in Western Blot (WB) — Western blot analysis of TET1 using A) 50 µg whole cell lysate from 293T cells transfected with scramble siRNA and B) 50 µg whole cell lysate from TET1-knockdowned 293T cells. Samples were loaded onto a 6% SDS-PAGE gel and probed with a TET1 monoclonal antibody (Product # MA5-16312) at a dilution of 1:500.

TET1 Antibody in Immunoprecipitation (IP) — Immunoprecipitation and Western blot of TET1 protein from NT2D1 whole cell extracts using 5 µg of TET1 antibody (Product # MA5-16312).

Product Details

Tested Applications

Dilution

ChIP assay (ChIP)

Assay Dependent

Immunocytochemistry (ICC)

1:100-1:1000

Immunofluorescence (IF)

1:100-1:1000

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1000

Immunoprecipitation (IP)

Assay Dependent

Western Blot (WB)

1:500-1:3000

Product Specifications

Species Reactivity

Human

Host / Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

GT1462

Immunogen

tet oncogene 1

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2537831

Product Specific Information

MA5-16312 targets TET1 in WB, ICC, IP, ChIP, IHC(P) and IF applications and shows reactivity with Human samples.

The MA5-16312 immunogen is tet oncogene 1.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.

Target Information

Dioxygenase that specifically binds methylcytosine (5mC), a minor base in mammalian DNA found in repetitive DNA elements that is crucial for retrotransposon silencing and mammalian development. Catalyzes the conversion of methylcytosine (5mC) to 5-hydroxymethylcytosine (hmC). The clear function of 5-hydroxymethylcytosine (hmC) is still unclear but it may influence chromatin structure and recruit specific factors or may constitute an intermediate component in cytosine demethylation. 5-hydroxymethylcytosine (hmC) is present in ES cells and is enriched in the brain, especially in Purkinje neurons. May play a role in the fetal development of heart, lung and brain.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: bA119F7.1; CXXC finger 6; CXXC zinc finger 6; CXXC-type zinc finger protein 6; CXXC6; LCX; Leukemia-associated protein with a CXXC domain; Methylcytosine dioxygenase TET1; Ten-eleven translocation 1 gene protein; ten-eleven translocation-1; tet oncogene 1; TET1 splice variant VP_DE4; TET1 splice variant VP_DE456

Gene Aliases: bA119F7.1; CXXC6; KIAA1676; LCX; MLL-TET1; TET1; TET1-MLL

UniProt ID: (Human) Q7Z6B6

Entrez Gene ID: (Human) 80312

Function(s)

inner cell mass cell differentiation transcription, DNA-templated protein O-linked glycosylation positive regulation of cell proliferation covalent chromatin modification stem cell population maintenance positive regulation of histone methylation positive regulation of transcription from RNA polymerase II promoter oxidation-reduction process DNA demethylation negative regulation of methylation-dependent chromatin silencing

Process(es)

iron ion binding zinc ion binding structure-specific DNA binding methylcytosine dioxygenase activity

TET1 Monoclonal Antibody (GT1462)

Product Details

Product Specific Information

Target Information

Bioinformatics

Material safety data sheets (MSDS)

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