Invitrogen

TET1 Monoclonal Antibody (GT1462)

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TET1 Antibody in Immunocytochemistry (ICC/IF) — TET1 Monoclonal Antibody (GT1462) detects TET1 protein at nucleus by immunofluorescent analysis. Sample: TET1-transfected (right) or untransfected (left) 293T cells were fixed in 4% paraformaldehyde for 15 min. Green: TET1 protein stained by TET1 antibody (Product # MA5-16312) diluted at 1:1,000. Blue: Hoechst 33342 staining. Scale bar = 10 µm.

TET1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — TET1 Monoclonal Antibody (GT1462) detects TET1 protein at nucleus on HeLa xenograft by immunohistochemical analysis. Sample: Paraffin-embedded HeLa xenograft. TET1 Monoclonal Antibody (GT1462) (Product # MA5-16312) dilution: 1:100. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

TET1 Antibody in Western Blot (WB) — TET1 Monoclonal Antibody (GT1462) detects TET1 protein by western blot analysis. A. 50 µg whole cell lysate/extract from 293T cells transfected with scramble siRNA. B. 50 µg whole cell lysate/extract from TET1-knockdowned 293T cells.6% SDS-PAGE. TET1 Monoclonal Antibody (GT1462) (Product # MA5-16312) dilution: 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

TET1 Antibody in Immunohistochemistry (IHC) — 1 Fig. TET1 expression is elevated in patients with CCA. (A) Heat map for the expression of 2-OG-dependent enzymes was generated using TCGA data, which include 9 normal samples, 6 CCA tumors with IDH1 mutation, and 30 CCA tumors with WT IDH1. (B) The association of molecular signaling pathways and functions with alpha-KG-dependent enzymes. (C) Relative TET1, TET2, and TET3 mRNA expression levels were analyzed in human normal bile ducts (n = 9) and patients with CCA (n = 36) using TCGA data. (D) Representative TET1 immunohistochemical images are shown in noncancerous bile duct (a) and CCA samples (b-d). (E) Semiquantification of TET1 immunohistochemical staining data was calculated using Image J software. ** P < 0.01, *** P < 0.001 compared with relevant control.

TET1 Antibody in Western Blot, Immunoprecipitation (WB, IP) — 5 Figure miR-376a directly targeted TET1 and HIF1A. (A) Bioinformatics prediction (TargetScan database) of putative binding site at 3'-UTRs of TET1 and HIF-1A by miR-376a. (B and C) Luciferase activity was measured in HGC-27 cells transfected with HIF-1A or TET1 3'-UTR-fused pGL3 vector plus NC mimic, miR-376a mimic, NC inhibitor, miR-376a inhibitor. * p < 0.05; ** p < 0.01. (D) RT-qPCR showed miR-376a, TET1, HIF-1A expression levels in HGC-27 cells transfected with NC mimic, miR-376a mimic, NC inhibitor, miR-376a inhibitor. * p < 0.05; ** p < 0.01. (E) Co-immunoprecipitation showed interaction of TET1 with HIF-1A in HGC-27 cells. IgG served as negative control. (F) RT-qPCR showed PD-L1 mRNA levels in HGC-27 cells transfected with shNC, shHIF-1A, shTET1, shHIF-1A plus shTET1. ** p < 0.01. (G) Flow cytometry analysis showed PD-L1 protein levels in HGC-27 cells transfected with shNC, shHIF-1A, shTET1, shHIF-1A plus shTET1

TET1 Antibody in ChIP assay (ChIP) — Figure 4. PROSER1 regulates the chromatin association of TET1/2 to mediate UTX/H3K4me1-dependent enhancer activity. (A) Heatmaps centered on 18,017 reproducible PROSER1 binding sites obtained from three different PROSER1 antibodies in WT HEK293 cells. Occupancy of PROSER1, UTX, OGT, TET1, TET2, H3K4me1, H3K4me2, H3K4me3, and H3K27ac is displayed. (B) Venn diagram depicting the overlap between reproducible PROSER1, UTX, and TET2 peaks in WT HEK293 cells. (C) Heatmaps centered on 4,421 UTX/H3K4me1-down-regulated regions in PROSER1 KO compared with WT cells. Occupancy of PROSER1, UTX, OGT, TET1, TET2, H3K4me1, H3K4me2, H3K4me3, and H3K27ac is displayed. (D) Genome browser tracks depicting the ChIP-seq profiles of PROSER1, UTX, OGT, TET1, TET2, H3K4me1, H3K4me2, H3K4me3, and H3K27ac in WT and PROSER1 KO HEK293 cells at the DDB2 promoter (left) and SOX2 enhancer (right). (E) Genome browser tracks showing the RNA-seq profiles of the DDB2 and SOX2 genes in WT and PROSER1 KO HEK293 cells. Two replicates are displayed for each genotype. (F) Violin plots showing transcriptional down-regulation of the 400 genes associated with the 4,421 regions displaying lower enrichment of UTX and H3K4me1 in PROSER1 KO compared with WT cells. Expression values were standardized for each gene across the samples. The median interquartile range and the 1.5x interquartile range are shown for each violin plot.

Product Details

MA5-16312

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:3,000	View 3 publications 3 publications
Immunohistochemistry (IHC)	-	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	1:100-1:1,000	-
Immunocytochemistry (ICC/IF)	1:100-1:1,000	-
Flow Cytometry (Flow)	-	View 1 publication 1 publication
Immunoprecipitation (IP)	-	View 2 publications 2 publications
ChIP assay (ChIP)	-	View 2 publications 2 publications

Product Specifications
Species Reactivity	Human, Mouse
Published species	Human, Mouse
Host/Isotype	Mouse / IgG2b
Class	Monoclonal
Type	Antibody
Clone	GT1462
Immunogen	Recombinant protein encompassing a sequence within the center region of human TET1.
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Protein G
Storage buffer	PBS
Contains	no preservative
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2537831

Product Specific Information

Recommended positive controls: 293T, DDDDK-human TET1-transfected 293T, NT2D1, NT2D1 nuclear extract.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Dioxygenase that specifically binds methylcytosine (5mC), a minor base in mammalian DNA found in repetitive DNA elements that is crucial for retrotransposon silencing and mammalian development. Catalyzes the conversion of methylcytosine (5mC) to 5-hydroxymethylcytosine (hmC). The clear function of 5-hydroxymethylcytosine (hmC) is still unclear but it may influence chromatin structure and recruit specific factors or may constitute an intermediate component in cytosine demethylation. 5-hydroxymethylcytosine (hmC) is present in ES cells and is enriched in the brain, especially in Purkinje neurons. May play a role in the fetal development of heart, lung and brain.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

PROSER1 mediates TET2 O-GlcNAcylation to regulate DNA demethylation on UTX-dependent enhancers and CpG islands.

Life science alliance

Wang X,Rosikiewicz W,Sedkov Y,Martinez T,Hansen BS,Schreiner P,Christensen J,Xu B,Pruett-Miller SM,Helin K,Herz HM

Published figure using TET1 monoclonal antibody (Product # MA5-16312) in ChIP assay

Sat Jan 01 00:00:00 EST 2022

PROSER1 mediates TET2 O-GlcNAcylation to regulate DNA demethylation on UTX-dependent enhancers and CpG islands.

Life science alliance

Wang X,Rosikiewicz W,Sedkov Y,Martinez T,Hansen BS,Schreiner P,Christensen J,Xu B,Pruett-Miller SM,Helin K,Herz HM

Published figure using TET1 monoclonal antibody (Product # MA5-16312) in ChIP assay

Sat Jan 01 00:00:00 EST 2022

Ten-Eleven Translocation 1 Promotes Malignant Progression of Cholangiocarcinoma With Wild-Type Isocitrate Dehydrogenase 1.

Hepatology (Baltimore, Md.)

Bai X,Zhang H,Zhou Y,Nagaoka K,Meng J,Ji C,Liu D,Dong X,Cao K,Mulla J,Cheng Z,Mueller W,Bay A,Hildebrand G,Lu S,Wallace J,Wands JR,Sun B,Huang CK

Published figure using TET1 monoclonal antibody (Product # MA5-16312) in Immunohistochemistry

Sat May 01 00:00:00 EDT 2021

LncRNA NUTM2A-AS1 positively modulates TET1 and HIF-1A to enhance gastric cancer tumorigenesis and drug resistance by sponging miR-376a.

Cancer medicine

Wang J,Yu Z,Wang J,Shen Y,Qiu J,Zhuang Z

MA5-16312 was used in Western Blotting to elucidate the molecular mechanism of the role of NUTM2A-AS1 in gastric cancer.

Tue Dec 01 00:00:00 EST 2020

LncRNA NUTM2A-AS1 positively modulates TET1 and HIF-1A to enhance gastric cancer tumorigenesis and drug resistance by sponging miR-376a.

Cancer medicine

Wang J,Yu Z,Wang J,Shen Y,Qiu J,Zhuang Z

MA5-16312 was used in Western Blotting to elucidate the molecular mechanism of the role of NUTM2A-AS1 in gastric cancer.

Tue Dec 01 00:00:00 EST 2020

LncRNA NUTM2A-AS1 positively modulates TET1 and HIF-1A to enhance gastric cancer tumorigenesis and drug resistance by sponging miR-376a.

Cancer medicine

Wang J,Yu Z,Wang J,Shen Y,Qiu J,Zhuang Z

MA5-16312 was used in Western Blotting to elucidate the molecular mechanism of the role of NUTM2A-AS1 in gastric cancer.

Tue Dec 01 00:00:00 EST 2020

Ligand-induced gene activation is associated with oxidative genome damage whose repair is required for transcription.

Proceedings of the National Academy of Sciences of the United States of America

Sengupta S,Wang H,Yang C,Szczesny B,Hegde ML,Mitra S

MA5-16312 was used in Immunoprecipitation to show induction of oxidized bases and single-strand breaks (SSBs), but not direct double-strand breaks (DSBs), in the genome during gene activation by ligands of the nuclear receptor superfamily.

Tue Sep 08 00:00:00 EDT 2020

Vitamin C supports conversion of human γδ T cells into FOXP3-expressing regulatory cells by epigenetic regulation.

Scientific reports

Kouakanou L,Peters C,Sun Q,Floess S,Bhat J,Huehn J,Kabelitz D

MA5-16312 was used in Flow Cytometry to investigate the influence of phospho-modified Vitamin C on the regulatory activity of human Vγ9 Vδ2 T cells and TGF-β-induced FOXP3 expression.

Thu Apr 16 00:00:00 EDT 2020

Integrin α6 signaling induces STAT3-TET3-mediated hydroxymethylation of genes critical for maintenance of glioma stem cells.

Oncogene

Herrmann A,Lahtz C,Song J,Aftabizadeh M,Cherryholmes GA,Xin H,Adamus T,Lee H,Grunert D,Armstrong B,Chu P,Brown C,Lim M,Forman S,Yu H

MA5-16312 was used in Western Blotting to conclude that ECM- integrin α6-STAT3-TET3 axis regulates hydroxymethylation of genes important for GSCs, thereby increasing GSC tumorigenicity and resistance to therapies.

Sun Mar 01 00:00:00 EST 2020

Hemodynamic disturbed flow induces differential DNA methylation of endothelial Kruppel-Like Factor 4 promoter in vitro and in vivo.

Circulation research

Jiang YZ,Jiménez JM,Ou K,McCormick ME,Zhang LD,Davies PF

Published figure using TET1 monoclonal antibody (Product # MA5-16312) in ChIP assay

Fri Jun 20 00:00:00 EDT 2014

Bioinformatics

Protein Aliases: CXXC finger 6; CXXC zinc finger 6; CXXC-type zinc finger protein 6; Leukemia-associated protein with a CXXC domain; Methylcytosine dioxygenase TET1; RP11-119F7.1; Ten-eleven translocation 1 gene protein; ten-eleven translocation 1 gene protein homolog; ten-eleven translocation-1; tet oncogene 1; TET1 splice variant VP_DE4; TET1 splice variant VP_DE456

Gene Aliases: 2510010B09Rik; AA517754; bA119F7.1; BB001228; CXXC6; D10Ertd17e; KIAA1676; LCX; mKIAA1676; MLL-TET1; TET1; TET1-MLL

UniProt ID: (Human) Q8NFU7

Entrez Gene ID: (Human) 80312, (Mouse) 52463

Function(s)