Western blot analysis of TF in (lane 2) Gene specific transient over-expression HEK293T cell lysate and (lane 3) Empty vector transfected control HEK293T cell lysate. Samples were probed with a TF polyclonal antibody (Product # PA1-27935) at a dilution of 1:1000. Secondary detection was performed using a peroxidase conjugated Rabbit anti-Chicken IgY (IgG) at a 1:10,000 dilution and developed with TMB Substrate.
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Chicken / IgY|
|Immunogen||Synthetic peptide corresponding to humanSerotransferrin.|
|Purification||Antigen affinity chromatography|
|Contains||0.01% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|ELISA (ELISA)||0.1 µg/ml|
|Western Blot (WB)||1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-27935 detects TF from human, mouse, and rat samples.
PA1-27935 may be successfully used in ELISA and Western blot applications. By Western blot, this antibody has only been tested on plasma transferrin protein.
The immunogen is a synthetic peptide corresponding to humanSerotransferrin.
Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion. usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.