|Tested species reactivity||Dog, Hamster, Human, Mouse, Rat|
|Published species reactivity||Not Applicable|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Recombinant full length protein (Human).|
|Storage buffer||0.05M sodium phosphate, pH 7.5, with 0.2% gelatin|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||1-5 µg/ml|
|Immunofluorescence (IF)||1-5 ug/ml|
|Immunohistochemistry (Frozen) (IHC (F))||1-5 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||1-10 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 1 publications below|
MA1-91686 detects TGF alpha from human, mouse, rat, canine and hamster samples. MA1-91686 does not cross react with human or mouse EGF.
MA1-91686 has been successfully used in immunocytochemistry, immunohistochemistry (frozen tissue), immunohistochemistry (paraffin tissue) applications. MA1-91686 is not suitable for use in Western blot applications.
The MA1-91686 immunogen is: Recombinant full length protein (Human).
Transforming growth factor-alpha (TGF alpha) is an acid- and heat-stable originally discovered in rodents and humans. TGF alpha protien is 33% homologous to epidermal growth factor (EGF). TGF alpha binds to the EGF receptor, mediating tyrosine phosphorylation of the receptor, and promotes anchorage-independent growth of normal rat fibroblasts in soft agar in the presence of transforming growth factor-beta. TGF alpha is secreted by a variety of transformed cells and tumors, embryonic cells and some normal adult cells . TGF alpha bioactivity has also been found in the urine of cancer patients. It has been suggested that it may act as an autocrine growth factor for the induction or maintenance of malignancy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
TGF¿ expression in myeloid malignancies.
MA1-91686 was used in immunohistochemistry to study myeloid malignancy in TGFalpha expression
|Kavanagh S,Mirzai B,Fuller K,Erber WN||Journal of clinical pathology (69:543)||2016|