|Tested species reactivity||Human|
|Published species reactivity||Human, Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||TGF-beta from human platelets|
|Storage buffer||PBS, pH 7|
|Contains||0.1% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||1:1000|
|Immunohistochemistry (Paraffin) (IHC (P))||1:1000|
|Western Blot (WB)||5-20 ng/mL|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody reacts with the dimeric (25 kDa) and monomeric (12.5 kDa) forms of TGF-beta under both non-reducing and reducing conditions. In ELISA applications, this antibody recognizes both human platelet-derived and recombinant TGF-beta.
This antibody neutralizes TGF-beta activity in vitro in an inhibition assay of CCL/64 cell growth, and neutralizes the growth promoting action of TGF-beta in the NRK-49F colony forming assay. The effect of microinjection of this antibody into one blastomere of two cell stage Xenopus embryos indicated that it was able to neutralize effectively the bioactivity of TGF-beta in vivo.
In IHC applications, this antibody has been used to demonstrate TGF-beta in ovine ovarian tissue and human breast carcinoma. As a consequence of the intense staining of the erythrocytes, it is possible to locate a single cell within the ovarian stroma making it useful in locating very fine capillary networks within tissue.
TGF beta (Transforming Growth Factor beta) is a multifunctional peptide that controls proliferation, differentiation and other functions in many cell types.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
IL-10 producing CD8+ T cells in human infection with Leishmania guyanensis.
MA5-18023 was used in blocking or activating experiment to measure TGF-beta, IL-10, and IFN-gamma production by peripheral blood mononuclear cells of unexposed naive subjects and LCL patients after stimulation with live Leishmania guyanensis
|Bourreau E,Ronet C,Couppié P,Sainte-Marie D,Tacchini-Cottier F,Launois P||Microbes and infection (9:1034)||2007|
|Not Applicable||Not Cited||
Transforming growth factor beta 1 production by CD4+ CD25+ regulatory T cells in peripheral blood mononuclear cells from healthy subjects stimulated with Leishmania guyanensis.
MA5-18023 was used in blocking or activating experiment to show that TGF-beta1 is involved in the suppressive activity of Leishmania guyanensis-stimulated CD4+ CD25+ T cells from healthy controls
|Kariminia A,Bourreau E,Pascalis H,Couppié P,Sainte-Marie D,Tacchini-Cottier F,Launois P||Infection and immunity (73:5908)||2005|
Tumor necrosis factor-alpha and angiostatin are mediators of endothelial cytotoxicity in bronchoalveolar lavages of patients with acute respiratory distress syndrome.
MA5-18023 was used in blocking assay to test if factors in the alveolar milieu induce cell death in human lung microvascular endothelial cells.
|Hamacher J,Lucas R,Lijnen HR,Buschke S,Dunant Y,Wendel A,Grau GE,Suter PM,Ricou B||American journal of respiratory and critical care medicine (166:651)||2002|