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Invitrogen
TID1 Monoclonal Antibody (RS13)
Advanced Verification
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 5
TID1 Antibody (MA5-12267) in ICC/IF
Immunofluorescence analysis of TID1 was performed using 70% confluent log phase HepG2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with TID1 (RS13) Mouse Monoclonal Antibody (Product # MA5-12267) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A281... View More
Product Details
MA5-12267
Applications
Tested Dilution
Publications
Product Specifications
Published species
Mouse
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
RS13
Immunogen
Recombinant hTid-1 protein
Conjugate
Form
Liquid
Concentration
0.2 mg/mL
Storage conditions
4° C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_10984230
Product Specific Information
MA5-12267 targets TID-1 in IP, IP, and WB applications and shows reactivity with Human, mouse, and Rat samples.^M
^M
The MA5-12267 immunogen is recombinant hTid-1 protein.
Target Information
TID1 is a human homolog of the Drosophila tumor suppressor lethal (2) tumorous imaginal discs, l(2)tid and encodes two mitochondrial matrix localized splice variants of human Tid-1 designated hTid-1S and hTid-1L. These proteins are the conserved members of the DnaJ family of proteins which act as cochaperones for mitochondrial Hsp70. They contain a conserved tetrahedrical J domain which binds to Hsp70 chaperones and activates their ATPase activity. Expression of hTid-1L increases apoptosis induced by DNA damaging agents as mitomycin-C and TNF-a. A J-domain mutant of hTid-1L can dominantly suppress apoptosis and in sharp contrast the J-domain mutant of hTid-1S increases apoptosis. Expression of hTid-1S and hTid-1L affects cytochrome c release from the mitochondria and caspase 3 activation, while activation of caspase 8 is unaffected. It is strongly suggested that these two splice variants exert their anti- and pro- apoptotic effects through discrete substrates and activities. Hence the relative abundance of these proteins or their substrates may allow the mitochondria to dampen or enhance the apoptotic signals.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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