Invitrogen

TIGIT Monoclonal Antibody (MBSA43), Functional Grade, eBioscience™

31 Published Figures

24 References

View all (75) TIGIT antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Antibody Testing Data (1)
Published Figures (31)

TIGIT Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 32

TIGIT Antibody (16-9500-82) in IHC (P)

Immunohistochemical analysis of TIGIT was performed using formalin-fixed paraffin-embedded Human tonsil tissue sections. To expose the target protein, heat-induced epitope retrieval was performed on de-paraffinized sections using eBioscience™ IHC Antigen Retrieval Solution - Low pH (10X) (Product # 00-4955-58) diluted to 1X solution in water in a decloaking chamber at 110 degrees Celsius for 15 minutes. Fol... View More

Product Image Gallery

TIGIT Antibody in Immunohistochemistry (IHC) — Fig. 6 Cell-to-cell communications between cancer cells and immune cells. A - F Heatmaps showing numbers of cell-cell interactions across cell types from variant sample origins. G, H NATMI networks showing ligand-receptor pairs among cells in lymph nodes with TNBC ( G ) and non-TNBC ( H ) cells. I Table showing impressive molecules and corresponding ligand-receptor pairs associated with breast cancer metastasis and the respective references. J Immunofluorescence staining of NECTIN2-TIGIT pair in lymph nodes with cancer cells.

TIGIT Antibody in Flow Cytometry (Flow) — Figure 4 TCF4 induces TIGIT expression on memory B cells. ( A ) Venn diagrams showing the overlapped genes. Significantly upregulated genes (log 2 FC >0.5, FDR < 0.1) in TIGIT + IL-10 - cells compared with TIGIT - IL-10 + cells and TIGIT - IL-10 - cells were evaluated. Among the 73 overlapped genes, transcription factors are highlighted, and heatmaps are depicted on basis of the log 2 FC under the IL-4-stimulated condition. *FDR < 0.1, **FDR < 0.01, and ***FDR < 0.001. ( B ) TIGIT + and TIGIT - cells were sorted from CD20 + CD27 + memory B cells stimulated with CD40L+IL-21 for 2 days, and TCF4 mRNA expression was measured relative to B2M by qPCR ( n = 6). Significance was determined by 2-tailed, unpaired Student's t test. ( C ) Representative histogram of TCF4 expression by flow cytometric analysis of TIGIT + cells, TIGIT - cells, and control (no staining). Max, maximum. ( D ) mRNA expression kinetics of TCF4 and TIGIT from 7 different time points ( n = 7). Data are presented as the mean +- SEM. ( E - G ) CD20 + CD27 + memory B cells were transfected with an siRNA targeting TCF4 (siTCF4) or the control (siCtrl). TCF4 expression was measured relative to B2M by qPCR, and 51% knockdown efficiency was confirmed ( E ). Representative flow data for TIGIT expression ( F ) and the proportion of TIGIT + cells ( G ). Data are presented as the mean +- SEM and were evaluated by 2-tailed, unpaired Student's t test ( E and G ).

Product Details

16-9500-82

Applications

Tested Dilution

Publications

Western Blot (WB)

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

0.25 µg/test

View 19 publications 19 publications

Functional Assay (FN)

Assay-Dependent

Inhibition Assays (IA)

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Functional Grade, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

MBSA43

Conjugate

Functional Grade

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

no preservative

Storage conditions

4° C

RRID

AB_10718831

Product Specific Information

Description: The MBSA43 monoclonal antibody reacts with human T cell immunoreceptor with Ig and ITIM domains (TIGIT), which is also known as VSTM3 or WUCAM. This 26 kDa protein is a member of the poliovirus receptor (PVR) family. The expression of TIGIT has been reported on NK cells, regulatory T cells, follicular T helper cells, memory CD4+ T cells, and CD8+ T cells, but it is not expressed on B cells or naive CD4+ T cells. TIGIT may be upregulated on naive CD4+ T cells upon activation. TIGIT can interact with certain members of the PVR and PVR-like families, including CD155, and also mediates the interaction of NK and T cells with antigen presenting cells, fibroblasts, and endothelial cells that express PVR and PVR-like proteins.

MBSA43 is reported to crossreact with Rhesus macaque.

Applications Reported: This MBSA43 antibody has been reported for use in flow cytometric analysis. In a functional assay, MBSA43 enhances IFN gamma secretion following TCR activation.

Applications Tested: This MBSA43 antibody has been tested by flow cytometric analysis. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Storage and handling: Use in a sterile environment.

Filtration: 0.2 µm post-manufacturing filtered.

Purity: Greater than 90%, as determined by SDS-PAGE.

Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.

Aggregation: Less than 10%, as determined by HPLC.

Target Information

Binds with high affinity to the poliovirus receptor (PVR) which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T cell activation by promoting the generation of mature immunoregulatory dendritic cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

TIGIT expressing CD4+T cells represent a tumor-supportive T cell subset in chronic lymphocytic leukemia.

Oncoimmunology

Catakovic K,Gassner FJ,Ratswohl C,Zaborsky N,Rebhandl S,Schubert M,Steiner M,Gutjahr JC,Pleyer L,Egle A,Hartmann TN,Greil R,Geisberger R

Published figure using TIGIT monoclonal antibody (Product # 16-9500-82) in Flow Cytometry

Sat Jan 29 00:00:00 EST 2022

Impaired TIGIT expression on B cells drives circulating follicular helper T cell expansion in multiple sclerosis.

The Journal of clinical investigation

Asashima H,Axisa PP,Pham THG,Longbrake EE,Ruff WE,Lele N,Cohen I,Raddassi K,Sumida TS,Hafler DA

Published figure using TIGIT monoclonal antibody (Product # 16-9500-82) in Flow Cytometry

Mon Oct 17 00:00:00 EDT 2022

Deep spatial profiling of human COVID-19 brains reveals neuroinflammation with distinct microanatomical microglia-T-cell interactions.

Immunity

Schwabenland M,Salié H,Tanevski J,Killmer S,Lago MS,Schlaak AE,Mayer L,Matschke J,Püschel K,Fitzek A,Ondruschka B,Mei HE,Boettler T,Neumann-Haefelin C,Hofmann M,Breithaupt A,Genc N,Stadelmann C,Saez-Rodriguez J,Bronsert P,Knobeloch KP,Blank T,Thimme R,Glatzel M,Prinz M,Bengsch B

16-9500-82 was used in Mass cytometry to identify profound neuroinflammation with activation of innate and adaptive immune cells as correlates of COVID-19 neuropathology, with implications for potential therapeutic strategies.

Tue Jul 13 00:00:00 EDT 2021

Single-cell RNA sequencing reveals cell heterogeneity and transcriptome profile of breast cancer lymph node metastasis.

Oncogenesis

Xu K,Wang R,Xie H,Hu L,Wang C,Xu J,Zhu C,Liu Y,Gao F,Li X,Wang C,Huang J,Zhou W,Zhou G,Shu Y,Guan X

Published figure using TIGIT monoclonal antibody (Product # 16-9500-82) in Immunocytochemistry

Tue Oct 05 00:00:00 EDT 2021

Correlations of the expression of γδ T cells and their co-stimulatory molecules TIGIT, PD-1, ICOS and BTLA with PR and PIBF in the peripheral blood and decidual tissues of women with unexplained recurrent spontaneous abortion.

Clinical and experimental immunology

Liang Q,Tong L,Xiang L,Shen S,Pan C,Liu C,Zhang H

Published figure using TIGIT monoclonal antibody (Product # 16-9500-82) in Flow Cytometry

Fri Jan 01 00:00:00 EST 2021

Mycophenolate mofetil enhances the effects of tacrolimus on the inhibitory function of regulatory T cells in patients after liver transplantation via PD-1 and TIGIT receptors.

Immunopharmacology and immunotoxicology

Zeng Q,Yuan X,Cao J,Zhao X,Wang Y,Liu B,Liu W,Zhu Z,Dou J

Published figure using TIGIT monoclonal antibody (Product # 16-9500-82) in Flow Cytometry

Thu Apr 01 00:00:00 EDT 2021

SARS-CoV-2-specific T cell memory is sustained in COVID-19 convalescent patients for 10 months with successful development of stem cell-like memory T cells.

Nature communications

Jung JH,Rha MS,Sa M,Choi HK,Jeon JH,Seok H,Park DW,Park SH,Jeong HW,Choi WS,Shin EC

Published figure using TIGIT monoclonal antibody (Product # 16-9500-82) in Flow Cytometry

Wed Jun 30 00:00:00 EDT 2021

TIGIT⁺ TIM-3⁺ NK cells are correlated with NK cell exhaustion and disease progression in patients with hepatitis B virus‑related hepatocellular carcinoma.

Oncoimmunology

Yu L,Liu X,Wang X,Yan F,Wang P,Jiang Y,Du J,Yang Z

Published figure using TIGIT monoclonal antibody (Product # 16-9500-82) in Flow Cytometry

Mon Aug 02 00:00:00 EDT 2021

TIGIT is upregulated by HIV-1 infection and marks a highly functional adaptive and mature subset of natural killer cells.

AIDS (London, England)

Vendrame E,Seiler C,Ranganath T,Zhao NQ,Vergara R,Alary M,Labbé AC,Guédou F,Poudrier J,Holmes S,Roger M,Blish CA

16-9500-82 was used in Inhibition Assays to investigate the mechanisms that govern natural killer (NK)-cell responses to HIV.

Fri May 01 00:00:00 EDT 2020

Methylome-based cell-of-origin modeling (Methyl-COOM) identifies aberrant expression of immune regulatory molecules in CLL.

Genome medicine

Wierzbinska JA,Toth R,Ishaque N,Rippe K,Mallm JP,Klett LC,Mertens D,Zenz T,Hielscher T,Seifert M,Küppers R,Assenov Y,Lutsik P,Stilgenbauer S,Roessner PM,Seiffert M,Byrd J,Oakes CC,Plass C,Lipka DB

Published figure using TIGIT monoclonal antibody (Product # 16-9500-82) in Flow Cytometry

Wed Mar 18 00:00:00 EDT 2020

Risk-associated alterations in marrow T cells in pediatric leukemia.

JCI insight

Bailur JK,McCachren SS,Pendleton K,Vasquez JC,Lim HS,Duffy A,Doxie DB,Kaushal A,Foster C,DeRyckere D,Castellino S,Kemp ML,Qiu P,Dhodapkar MV,Dhodapkar KM

Published figure using TIGIT monoclonal antibody (Product # 16-9500-82) in Flow Cytometry

Thu Aug 20 00:00:00 EDT 2020

Imbalance of the CD226/TIGIT Immune Checkpoint Is Involved in the Pathogenesis of Primary Biliary Cholangitis.

Frontiers in immunology

Deng C,Li W,Fei Y,Wang L,Chen Y,Zeng X,Zhang F,Li Y

16-9500 was used in Flow cytometry/Cell sorting to look at PBC patients (n = 42) showing significantly higher proportions of peripheral CD8+ T and CD4+ T cells expressing either CD226 or TIGIT than disease (n = 25) and healthy (n = 30) controls.

Mon Apr 19 00:00:00 EDT 2021

A Comprehensive Analysis of Key Immune Checkpoint Receptors on Tumor-Infiltrating T Cells From Multiple Types of Cancer.

Frontiers in oncology

Li X,Wang R,Fan P,Yao X,Qin L,Peng Y,Ma M,Asley N,Chang X,Feng Y,Hu Y,Zhang Y,Li C,Fanning G,Jones S,Verrill C,Maldonado-Perez D,Sopp P,Waugh C,Taylor S,Mcgowan S,Cerundolo V,Conlon C,McMichael A,Lu S,Wang X,Li N,Dong T

Published figure using TIGIT monoclonal antibody (Product # 16-9500-82) in Flow Cytometry

Sun Jan 10 00:00:00 EST 2021

TIGIT signaling restores suppressor function of Th1 Tregs.

JCI insight

Lucca LE,Axisa PP,Singer ER,Nolan NM,Dominguez-Villar M,Hafler DA

16-9500 was used in Flow cytometry/Cell sorting to demonstrate an important role for TIGIT in controlling the functional stability of Tregs through repression of Akt, suggesting that the TIGIT pathway could be targeted for immunomodulatory therapies in human autoimmune disorders.

Thu Feb 07 00:00:00 EST 2019

Optimized RNP transfection for highly efficient CRISPR/Cas9-mediated gene knockout in primary T cells.

The Journal of experimental medicine

Seki A,Rutz S

16-9500 was used in Flow cytometry/Cell sorting to develop an optimised approach for Cas9/RNP transfection of primary mouse and human T cells without TCR stimulation, that results in near complete loss of target gene expression at the population level.

Mon Mar 05 00:00:00 EST 2018

PD-1 Blockade Promotes Emerging Checkpoint Inhibitors in Enhancing T Cell Responses to Allogeneic Dendritic Cells.

Frontiers in immunology

Stecher C,Battin C,Leitner J,Zettl M,Grabmeier-Pfistershammer K,Höller C,Zlabinger GJ,Steinberger P

16-9500 was used in Flow cytometry/Cell sorting to use human T cells stimulated with allogeneic dendritic cells were used to compare immune checkpoint inhibitors targeting TIM-3, BTLA, LAG-3, CTLA-4 and TIGIT alone or in combination with a PD-1 antibody.

Wed Mar 30 00:00:00 EDT 2022

HTLV-1 bZIP Factor Enhances T-Cell Proliferation by Impeding the Suppressive Signaling of Co-inhibitory Receptors.

PLoS pathogens

Kinosada H,Yasunaga JI,Shimura K,Miyazato P,Onishi C,Iyoda T,Inaba K,Matsuoka M

16-9500 was used in Flow cytometry/Cell sorting to study how HTLV-1 bZIP factor influences the expression and function of multiple co-inhibitory receptors to induce proliferation of infected T-cells.

Sun Jan 01 00:00:00 EST 2017

OMIP-037: 16-color panel to measure inhibitory receptor signatures from multiple human immune cell subsets.

Cytometry. Part A : the journal of the International Society for Analytical Cytology

Belkina AC,Snyder-Cappione JE

Published figure using TIGIT monoclonal antibody (Product # 16-9500-82) in Flow Cytometry

Wed Feb 01 00:00:00 EST 2017

Atorvastatin downregulates co-inhibitory receptor expression by targeting Ras-activated mTOR signalling.

Oncotarget

Okoye I,Namdar A,Xu L,Crux N,Elahi S

16-9500 was used in Flow cytometry/Cell sorting to suggest that statins may hold particular promise in reinvigorating T cell function in chronic conditions.

Fri Nov 17 00:00:00 EST 2017

TIGIT Marks Exhausted T Cells, Correlates with Disease Progression, and Serves as a Target for Immune Restoration in HIV and SIV Infection.

PLoS pathogens

Chew GM,Fujita T,Webb GM,Burwitz BJ,Wu HL,Reed JS,Hammond KB,Clayton KL,Ishii N,Abdel-Mohsen M,Liegler T,Mitchell BI,Hecht FM,Ostrowski M,Shikuma CM,Hansen SG,Maurer M,Korman AJ,Deeks SG,Sacha JB,Ndhlovu LC

Fri Jan 01 00:00:00 EST 2016

Effect of chorioamnionitis on regulatory T cells in moderate/late preterm neonates.

Human immunology

Rueda CM,Wells CB,Gisslen T,Jobe AH,Kallapur SG,Chougnet CA

Thu Jan 01 00:00:00 EST 2015

Nitric oxide donors increase PVR/CD155 DNAM-1 ligand expression in multiple myeloma cells: role of DNA damage response activation.

BMC cancer

Fionda C,Abruzzese MP,Zingoni A,Soriani A,Ricci B,Molfetta R,Paolini R,Santoni A,Cippitelli M

16-9500 was used in Immunofluorescence to explore the anti-myeloma activity of nitric oxide donors.

Thu Jan 22 00:00:00 EST 2015

Nitric oxide donors increase PVR/CD155 DNAM-1 ligand expression in multiple myeloma cells: role of DNA damage response activation.

BMC cancer

Fionda C,Abruzzese MP,Zingoni A,Soriani A,Ricci B,Molfetta R,Paolini R,Santoni A,Cippitelli M

16-9500 was used in Immunofluorescence to explore the anti-myeloma activity of nitric oxide donors.

Thu Jan 22 00:00:00 EST 2015

The TIGIT/CD226 axis regulates human T cell function.

Journal of immunology (Baltimore, Md. : 1950)

Lozano E,Dominguez-Villar M,Kuchroo V,Hafler DA

16-9500 was used in Flow cytometry/Cell sorting to demonstrate that TIGIT can inhibit T cell functions by competing with CD226 and can also directly inhibit T cells in a T cell-intrinsic manner.

Sun Apr 15 00:00:00 EDT 2012

Bioinformatics

Protein Aliases: T cell immunoreceptor with Ig and ITIM domains; T-cell immunoreceptor with Ig and ITIM domains; V-set and immunoglobulin domain containing 9; V-set and immunoglobulin domain-containing protein 9; V-set and transmembrane domain containing 3; V-set and transmembrane domain-containing protein 3; Washington University cell adhesion molecule

Gene Aliases: TIGIT; VSIG9; VSTM3; WUCAM

UniProt ID: (Human) Q495A1

Entrez Gene ID: (Human) 201633

Function(s)

homophilic cell adhesion via plasma membrane adhesion molecules heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules cell recognition negative regulation of interleukin-12 production positive regulation of interleukin-10 production negative regulation of T cell activation

Process(es)

receptor activity receptor binding protein binding protein homodimerization activity cell adhesion molecule binding

TIGIT Monoclonal Antibody (MBSA43), Functional Grade, eBioscience™

TIGIT Antibody (16-9500-82) in IHC (P)

Product Details

16-9500-82

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Functional Assay (FN)

Inhibition Assays (IA)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!