Invitrogen

TIGIT Monoclonal Antibody (MBSA43), Functional Grade, eBioscience™

Product Image Gallery

TIGIT Antibody in Flow Cytometry (Flow) — 3 Fig. The proportion of gammadelta T cells and the expression of their co-stimulatory molecules T cell immunoglobulin and ITIM domain (TIGIT), inducible co-stimulator (ICOS), B and T lymphocyte attenuator (BTLA) and programmed cell death 1 (PD-1) as determined by flow cytometry. The data are expressed as the mean +- standard error of the mean (s.e.m.). (a-e) Peripheral blood; normal pregnant (NP) group, n = 30; unexplained recurrent spontaneous abortion (URSA) group, n = 32; n.s. = not significant. (f-j) Decidual tissue; NP group, n = 11; URSA group, n = 15.

Product Details

16-9500-82

Applications	Tested Dilution	Publications
Western Blot (WB)	-	View 1 publication 1 publication
Immunocytochemistry (ICC/IF)	-	View 1 publication 1 publication
Flow Cytometry (Flow)	0.25 µg/test	View 15 publications 15 publications
Functional Assay (FN)	Assay-Dependent	-
Miscellaneous PubMed (Misc)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human
Published species	Human, Mouse
Host/Isotype	Mouse / IgG1, kappa
Recommended Isotype Control	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Functional Grade, eBioscience™
Class	Monoclonal
Type	Antibody
Clone	MBSA43
Conjugate	Functional Grade Alexa Fluor 488 Alexa Fluor 647 Alexa Fluor 660 Alexa Fluor 700 APC eFluor 450 FITC NovaFluor Red 700 PE PE-Cyanine7 PE-eFluor 610 PerCP-eFluor 710
Form	Liquid
Concentration	1 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2
Contains	no preservative
Storage conditions	4° C
RRID	AB_10718831

Product Specific Information

Description: The MBSA43 monoclonal antibody reacts with human T cell immunoreceptor with Ig and ITIM domains (TIGIT), which is also known as VSTM3 or WUCAM. This 26 kDa protein is a member of the poliovirus receptor (PVR) family. The expression of TIGIT has been reported on NK cells, regulatory T cells, follicular T helper cells, memory CD4+ T cells, and CD8+ T cells, but it is not expressed on B cells or naive CD4+ T cells. TIGIT may be upregulated on naive CD4+ T cells upon activation. TIGIT can interact with certain members of the PVR and PVR-like families, including CD155, and also mediates the interaction of NK and T cells with antigen presenting cells, fibroblasts, and endothelial cells that express PVR and PVR-like proteins.

MBSA43 is reported to crossreact with Rhesus macaque.

Applications Reported: This MBSA43 antibody has been reported for use in flow cytometric analysis. In a functional assay, MBSA43 enhances IFN gamma secretion following TCR activation.

Applications Tested: This MBSA43 antibody has been tested by flow cytometric analysis. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Storage and handling: Use in a sterile environment.

Filtration: 0.2 µm post-manufacturing filtered.

Purity: Greater than 90%, as determined by SDS-PAGE.

Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.

Aggregation: Less than 10%, as determined by HPLC.

Target Information

Binds with high affinity to the poliovirus receptor (PVR) which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T cell activation by promoting the generation of mature immunoregulatory dendritic cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

PD-1 Blockade Promotes Emerging Checkpoint Inhibitors in Enhancing T Cell Responses to Allogeneic Dendritic Cells.

Frontiers in immunology

Stecher C,Battin C,Leitner J,Zettl M,Grabmeier-Pfistershammer K,Höller C,Zlabinger GJ,Steinberger P

16-9500 was used in Flow cytometry/Cell sorting to use human T cells stimulated with allogeneic dendritic cells were used to compare immune checkpoint inhibitors targeting TIM-3, BTLA, LAG-3, CTLA-4 and TIGIT alone or in combination with a PD-1 antibody.

Wed Mar 30 00:00:00 EDT 2022

TIGIT expressing CD4+T cells represent a tumor-supportive T cell subset in chronic lymphocytic leukemia.

Oncoimmunology

Catakovic K,Gassner FJ,Ratswohl C,Zaborsky N,Rebhandl S,Schubert M,Steiner M,Gutjahr JC,Pleyer L,Egle A,Hartmann TN,Greil R,Geisberger R

Published figure using TIGIT monoclonal antibody (Product # 16-9500-82) in Flow Cytometry

Sat Jan 29 00:00:00 EST 2022

Deep spatial profiling of human COVID-19 brains reveals neuroinflammation with distinct microanatomical microglia-T-cell interactions.

Immunity

Schwabenland M,Salié H,Tanevski J,Killmer S,Lago MS,Schlaak AE,Mayer L,Matschke J,Püschel K,Fitzek A,Ondruschka B,Mei HE,Boettler T,Neumann-Haefelin C,Hofmann M,Breithaupt A,Genc N,Stadelmann C,Saez-Rodriguez J,Bronsert P,Knobeloch KP,Blank T,Thimme R,Glatzel M,Prinz M,Bengsch B

16-9500-82 was used in Mass cytometry to identify profound neuroinflammation with activation of innate and adaptive immune cells as correlates of COVID-19 neuropathology, with implications for potential therapeutic strategies.

Tue Jul 13 00:00:00 EDT 2021

Imbalance of the CD226/TIGIT Immune Checkpoint Is Involved in the Pathogenesis of Primary Biliary Cholangitis.

Frontiers in immunology

Deng C,Li W,Fei Y,Wang L,Chen Y,Zeng X,Zhang F,Li Y

16-9500 was used in Flow cytometry/Cell sorting to look at PBC patients (n = 42) showing significantly higher proportions of peripheral CD8+ T and CD4+ T cells expressing either CD226 or TIGIT than disease (n = 25) and healthy (n = 30) controls.

Mon Apr 19 00:00:00 EDT 2021

A Comprehensive Analysis of Key Immune Checkpoint Receptors on Tumor-Infiltrating T Cells From Multiple Types of Cancer.

Frontiers in oncology

Li X,Wang R,Fan P,Yao X,Qin L,Peng Y,Ma M,Asley N,Chang X,Feng Y,Hu Y,Zhang Y,Li C,Fanning G,Jones S,Verrill C,Maldonado-Perez D,Sopp P,Waugh C,Taylor S,Mcgowan S,Cerundolo V,Conlon C,McMichael A,Lu S,Wang X,Li N,Dong T

Published figure using TIGIT monoclonal antibody (Product # 16-9500-82) in Flow Cytometry

Sun Jan 10 00:00:00 EST 2021

Correlations of the expression of γδ T cells and their co-stimulatory molecules TIGIT, PD-1, ICOS and BTLA with PR and PIBF in the peripheral blood and decidual tissues of women with unexplained recurrent spontaneous abortion.

Clinical and experimental immunology

Liang Q,Tong L,Xiang L,Shen S,Pan C,Liu C,Zhang H

Published figure using TIGIT monoclonal antibody (Product # 16-9500-82) in Flow Cytometry

Fri Jan 01 00:00:00 EST 2021

Risk-associated alterations in marrow T cells in pediatric leukemia.

JCI insight

Bailur JK,McCachren SS,Pendleton K,Vasquez JC,Lim HS,Duffy A,Doxie DB,Kaushal A,Foster C,DeRyckere D,Castellino S,Kemp ML,Qiu P,Dhodapkar MV,Dhodapkar KM

Published figure using TIGIT monoclonal antibody (Product # 16-9500-82) in Flow Cytometry

Thu Aug 20 00:00:00 EDT 2020

Methylome-based cell-of-origin modeling (Methyl-COOM) identifies aberrant expression of immune regulatory molecules in CLL.

Genome medicine

Wierzbinska JA,Toth R,Ishaque N,Rippe K,Mallm JP,Klett LC,Mertens D,Zenz T,Hielscher T,Seifert M,Küppers R,Assenov Y,Lutsik P,Stilgenbauer S,Roessner PM,Seiffert M,Byrd J,Oakes CC,Plass C,Lipka DB

Published figure using TIGIT monoclonal antibody (Product # 16-9500-82) in Flow Cytometry

Wed Mar 18 00:00:00 EDT 2020

TIGIT signaling restores suppressor function of Th1 Tregs.

JCI insight

Lucca LE,Axisa PP,Singer ER,Nolan NM,Dominguez-Villar M,Hafler DA

16-9500 was used in Flow cytometry/Cell sorting to demonstrate an important role for TIGIT in controlling the functional stability of Tregs through repression of Akt, suggesting that the TIGIT pathway could be targeted for immunomodulatory therapies in human autoimmune disorders.

Thu Feb 07 00:00:00 EST 2019

Optimized RNP transfection for highly efficient CRISPR/Cas9-mediated gene knockout in primary T cells.

The Journal of experimental medicine

Seki A,Rutz S

16-9500 was used in Flow cytometry/Cell sorting to develop an optimised approach for Cas9/RNP transfection of primary mouse and human T cells without TCR stimulation, that results in near complete loss of target gene expression at the population level.

Mon Mar 05 00:00:00 EST 2018

Atorvastatin downregulates co-inhibitory receptor expression by targeting Ras-activated mTOR signalling.

Oncotarget

Okoye I,Namdar A,Xu L,Crux N,Elahi S

16-9500 was used in Flow cytometry/Cell sorting to suggest that statins may hold particular promise in reinvigorating T cell function in chronic conditions.

Fri Nov 17 00:00:00 EST 2017

OMIP-037: 16-color panel to measure inhibitory receptor signatures from multiple human immune cell subsets.

Cytometry. Part A : the journal of the International Society for Analytical Cytology

Belkina AC,Snyder-Cappione JE

Published figure using TIGIT monoclonal antibody (Product # 16-9500-82) in Flow Cytometry

Wed Feb 01 00:00:00 EST 2017

HTLV-1 bZIP Factor Enhances T-Cell Proliferation by Impeding the Suppressive Signaling of Co-inhibitory Receptors.

PLoS pathogens

Kinosada H,Yasunaga JI,Shimura K,Miyazato P,Onishi C,Iyoda T,Inaba K,Matsuoka M

16-9500 was used in Flow cytometry/Cell sorting to study how HTLV-1 bZIP factor influences the expression and function of multiple co-inhibitory receptors to induce proliferation of infected T-cells.

Sun Jan 01 00:00:00 EST 2017

TIGIT Marks Exhausted T Cells, Correlates with Disease Progression, and Serves as a Target for Immune Restoration in HIV and SIV Infection.

PLoS pathogens

Chew GM,Fujita T,Webb GM,Burwitz BJ,Wu HL,Reed JS,Hammond KB,Clayton KL,Ishii N,Abdel-Mohsen M,Liegler T,Mitchell BI,Hecht FM,Ostrowski M,Shikuma CM,Hansen SG,Maurer M,Korman AJ,Deeks SG,Sacha JB,Ndhlovu LC

Fri Jan 01 00:00:00 EST 2016

Nitric oxide donors increase PVR/CD155 DNAM-1 ligand expression in multiple myeloma cells: role of DNA damage response activation.

BMC cancer

Fionda C,Abruzzese MP,Zingoni A,Soriani A,Ricci B,Molfetta R,Paolini R,Santoni A,Cippitelli M

16-9500 was used in Immunofluorescence to explore the anti-myeloma activity of nitric oxide donors.

Thu Jan 22 00:00:00 EST 2015

Nitric oxide donors increase PVR/CD155 DNAM-1 ligand expression in multiple myeloma cells: role of DNA damage response activation.

BMC cancer

Fionda C,Abruzzese MP,Zingoni A,Soriani A,Ricci B,Molfetta R,Paolini R,Santoni A,Cippitelli M

16-9500 was used in Immunofluorescence to explore the anti-myeloma activity of nitric oxide donors.

Thu Jan 22 00:00:00 EST 2015

Effect of chorioamnionitis on regulatory T cells in moderate/late preterm neonates.

Human immunology

Rueda CM,Wells CB,Gisslen T,Jobe AH,Kallapur SG,Chougnet CA

Thu Jan 01 00:00:00 EST 2015

The TIGIT/CD226 axis regulates human T cell function.

Journal of immunology (Baltimore, Md. : 1950)

Lozano E,Dominguez-Villar M,Kuchroo V,Hafler DA

16-9500 was used in Flow cytometry/Cell sorting to demonstrate that TIGIT can inhibit T cell functions by competing with CD226 and can also directly inhibit T cells in a T cell-intrinsic manner.

Sun Apr 15 00:00:00 EDT 2012

Bioinformatics

Protein Aliases: T cell immunoreceptor with Ig and ITIM domains; T-cell immunoreceptor with Ig and ITIM domains; V-set and immunoglobulin domain containing 9; V-set and immunoglobulin domain-containing protein 9; V-set and transmembrane domain containing 3; V-set and transmembrane domain-containing protein 3; Washington University cell adhesion molecule

Gene Aliases: TIGIT; VSIG9; VSTM3; WUCAM

UniProt ID: (Human) Q495A1

Entrez Gene ID: (Human) 201633

Function(s)

homophilic cell adhesion via plasma membrane adhesion molecules heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules cell recognition negative regulation of interleukin-12 production positive regulation of interleukin-10 production negative regulation of T cell activation

Process(es)

receptor activity receptor binding protein binding protein homodimerization activity cell adhesion molecule binding