Invitrogen

TIGIT Monoclonal Antibody (MBSA43), PerCP-eFluor™ 710, eBioscience™

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TIGIT Antibody (46-9500-41) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD56 (NCAM) APC (Product # 17-0567-42) and Mouse IgG1 K Isotype Control PerCP-eFluor® 710 (Product # 46-4714-82) (left) or Anti-Human TIGIT PerCP-eFluor® 710 (right). Cells in the lymphocyte gate were used for analysis.

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Product Details

46-9500-41

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.125 µg)/test

View 11 publications 11 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PerCP-eFluor™ 710, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

MBSA43

Conjugate

PerCP-eFluor™ 710 PerCP-eFluor™ 710 PerCP-eFluor™ 710

Excitation/Emission Max

482/708 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10854428

Product Specific Information

Description: The MBSA43 monoclonal antibody reacts with human T cell immunoreceptor with Ig and ITIM domains (TIGIT), which is also known as VSTM3 or WUCAM. This 26 kDa protein is a member of the poliovirus receptor (PVR) family. The expression of TIGIT has been reported on NK cells, regulatory T cells, follicular T helper cells, memory CD4+ T cells, and CD8+ T cells, but it is not expressed on B cells or naive CD4+ T cells. TIGIT may be upregulated on naive CD4+ T cells upon activation. TIGIT can interact with certain members of the PVR and PVR-like families, including CD155, and also mediates the interaction of NK and T cells with antigen presenting cells, fibroblasts, and endothelial cells that express PVR and PVR-like proteins.

MBSA43 is reported to crossreact with Rhesus macaque.

Applications Reported: This MBSA43 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This MBSA43 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Binds with high affinity to the poliovirus receptor (PVR) which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T cell activation by promoting the generation of mature immunoregulatory dendritic cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Optimization of the proliferation and persistency of CAR T cells derived from human induced pluripotent stem cells.

Nature biomedical engineering

Ueda T,Shiina S,Iriguchi S,Terakura S,Kawai Y,Kabai R,Sakamoto S,Watanabe A,Ohara K,Wang B,Xu H,Minagawa A,Hotta A,Woltjen K,Uemura Y,Kodama Y,Seno H,Nakatsura T,Tamada K,Kaneko S

46-9500-42 was used in Flow cytometry/Cell sorting to show that the proliferation and persistency of the effector cells in the tumours can be further enhanced by genetically knocking out diacylglycerol kinase, which inhibits antigen-receptor signalling, and by transducing the cells with genes encoding for membrane-bound interleukin-15 (IL-15) and its receptor subunit IL-15Rα.

Sun Jan 01 00:00:00 EST 2023

Efficient clinical-grade γ-retroviral vector purification by high-speed centrifugation for CAR T cell manufacturing.

Molecular therapy. Methods & clinical development

Mekkaoui L,Tejerizo JG,Abreu S,Rubat L,Nikoniuk A,Macmorland W,Horlock C,Matsumoto S,Williams S,Smith K,Price J,Srivastava S,Hussain R,Banani MA,Day W,Stevenson E,Madigan M,Chen J,Khinder R,Miah S,Walker S,Ade-Onojobi M,Domining S,Sillibourne J,Sabatino M,Slepushkin V,Farzaneh F,Pule M

46-9500-42 was used in Flow cytometry/Cell sorting to report a simple one-step purification method by high-speed centrifugation for γ-RV produced by transient transfection for clinical application.

Thu Mar 09 00:00:00 EST 2023

CD19-CAR T cells undergo exhaustion DNA methylation programming in patients with acute lymphoblastic leukemia.

Cell reports

Zebley CC,Brown C,Mi T,Fan Y,Alli S,Boi S,Galletti G,Lugli E,Langfitt D,Metais JY,Lockey T,Meagher M,Triplett B,Talleur AC,Gottschalk S,Youngblood B

46-9500-42 was used in Flow cytometry/Cell sorting to demonstrate that CD19-CAR T cells undergo exhaustion-associated DNA methylation programming, indicating that efforts to prevent this process may be an attractive approach to improve CAR T cell efficacy.

Tue Nov 30 00:00:00 EST 2021

Homeostatic Function and Inflammatory Activation of Ileal CD8⁺ Tissue-Resident T Cells Is Dependent on Mucosal Location.

Cellular and molecular gastroenterology and hepatology

Lutter L,Roosenboom B,Brand EC,Ter Linde JJ,Oldenburg B,van Lochem EG,Horjus Talabur Horje CS,van Wijk F

46-9500-42 was used in Flow Cytometry to indicate that therapeutic strategies could have a different impact on the same immune cells depending on the local compartment and presence of an inflammatory milieu, and should be taken into account when investigating short- and long-term effects of new gut T cell-targeting drugs.

Mon Mar 28 00:00:00 EDT 2022

Beta cell-specific CD8⁺ T cells maintain stem cell memory-associated epigenetic programs during type 1 diabetes.

Nature immunology

Abdelsamed HA,Zebley CC,Nguyen H,Rutishauser RL,Fan Y,Ghoneim HE,Crawford JC,Alfei F,Alli S,Ribeiro SP,Castellaw AH,McGargill MA,Jin H,Boi SK,Speake C,Serti E,Turka LA,Busch ME,Stone M,Deeks SG,Sekaly RP,Zehn D,James EA,Nepom GT,Youngblood B

46-9500-42 was used in Flow Cytometry to investigate the long-lived nature of these cells, we established a DNA methylation-based T cell 'multipotency index' and found that beta cell-specific CD8+ T cells retained a stem-like epigenetic multipotency score.

Fri May 01 00:00:00 EDT 2020

Progression of mycosis fungoides occurs through divergence of tumor immunophenotype by differential expression of HLA-DR.

Blood advances

Murray D,McMurray JL,Eldershaw S,Pearce H,Davies N,Scarisbrick JJ,Moss P

Tue Feb 26 00:00:00 EST 2019

Targeting the mTOR pathway uncouples the efficacy and toxicity of PD-1 blockade in renal transplantation.

Nature communications

Esfahani K,Al-Aubodah TA,Thebault P,Lapointe R,Hudson M,Johnson NA,Baran D,Bhulaiga N,Takano T,Cailhier JF,Piccirillo CA,Miller WH

46-9500-42 was used in Flow Cytometry to indicate that sirolimus has the potential to uncouple anti-PD-1 therapy toxicity and efficacy.

Thu Oct 17 00:00:00 EDT 2019

CD226 opposes TIGIT to disrupt Tregs in melanoma.

JCI insight

Fourcade J,Sun Z,Chauvin JM,Ka M,Davar D,Pagliano O,Wang H,Saada S,Menna C,Amin R,Sander C,Kirkwood JM,Korman AJ,Zarour HM

46-9500 was used in Flow cytometry/Cell sorting to study if a high TIGIT/CD226 ratio in Tregs regulate their suppressive function and stability in melanoma.

Thu Jul 26 00:00:00 EDT 2018

CD4+ T Cells Expressing PD-1, TIGIT and LAG-3 Contribute to HIV Persistence during ART.

PLoS pathogens

Fromentin R,Bakeman W,Lawani MB,Khoury G,Hartogensis W,DaFonseca S,Killian M,Epling L,Hoh R,Sinclair E,Hecht FM,Bacchetti P,Deeks SG,Lewin SR,Sékaly RP,Chomont N

46-9500 was used in Flow cytometry/Cell sorting to investigate the contribution of PD-1, TIGIT and LAG-3 expressed on CD4+ T cells to HIV persistence during antiretroviral therapy.

Fri Jul 01 00:00:00 EDT 2016

TIGIT Marks Exhausted T Cells, Correlates with Disease Progression, and Serves as a Target for Immune Restoration in HIV and SIV Infection.

PLoS pathogens

Chew GM,Fujita T,Webb GM,Burwitz BJ,Wu HL,Reed JS,Hammond KB,Clayton KL,Ishii N,Abdel-Mohsen M,Liegler T,Mitchell BI,Hecht FM,Ostrowski M,Shikuma CM,Hansen SG,Maurer M,Korman AJ,Deeks SG,Sacha JB,Ndhlovu LC

Fri Jan 01 00:00:00 EST 2016

TIGIT Marks Exhausted T Cells, Correlates with Disease Progression, and Serves as a Target for Immune Restoration in HIV and SIV Infection.

PLoS pathogens

Fri Jan 01 00:00:00 EST 2016

Divergent Phenotypes of Human Regulatory T Cells Expressing the Receptors TIGIT and CD226.

Journal of immunology (Baltimore, Md. : 1950)

Fuhrman CA,Yeh WI,Seay HR,Saikumar Lakshmi P,Chopra G,Zhang L,Perry DJ,McClymont SA,Yadav M,Lopez MC,Baker HV,Zhang Y,Li Y,Whitley M,von Schack D,Atkinson MA,Bluestone JA,Brusko TM

46-9500 was used in Flow cytometry/Cell sorting to provide markers to delineate functionally distinct Treg subsets that may help direct cellular therapies and provide phenotypic markers for assessing their role in disease.

Wed Jul 01 00:00:00 EDT 2015

Bioinformatics

Protein Aliases: T cell immunoreceptor with Ig and ITIM domains; T-cell immunoreceptor with Ig and ITIM domains; V-set and immunoglobulin domain containing 9; V-set and immunoglobulin domain-containing protein 9; V-set and transmembrane domain containing 3; V-set and transmembrane domain-containing protein 3; Washington University cell adhesion molecule

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Gene Aliases: TIGIT; VSIG9; VSTM3; WUCAM

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UniProt ID: (Human) Q495A1

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Entrez Gene ID: (Human) 201633

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Function(s)

receptor activity receptor binding protein binding protein homodimerization activity cell adhesion molecule binding

Process(es)

homophilic cell adhesion via plasma membrane adhesion molecules heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules cell recognition negative regulation of interleukin-12 production positive regulation of interleukin-10 production negative regulation of T cell activation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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TIGIT Monoclonal Antibody (MBSA43), PerCP-eFluor™ 710, eBioscience™

TIGIT Antibody (46-9500-41) in Flow

Product Details

46-9500-41

Flow Cytometry (Flow)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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