Staining of Fc-blocked mouse resident peritoneal exudate cells with Anti-Mouse F4/80 Antigen eFluor® 660 (Product # 50-4801) and 0.125 µg of Rat IgG2a K Isotype Control PE (Product # 12-4321) (left) or 0.125 µg of Anti-Mouse TIM-4 PE (right). Cells in the large scatter population were used for analysis.
|Tested species reactivity||Mouse|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rat / IgG2a, kappa|
|Storage buffer||PBS, pH 7.2, with 0.1% gelatin|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C, store in dark, DO NOT FREEZE!|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||0.25 µg/test|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 3 publications below|
Description: The monoclonal antibody 54 recognizes mouse Tim-4 (T cell, Ig, mucin). Unlike other Tim family members, Tim-4 is not expressed on T lymphocytes but rather found on antigen presenting cells such as lymphoid and myeloid DC's and macrophages. Tim-4 has been shown to interact with Tim-1 thereby suggesting a role of Tim-4 in the balance of Th1/Th2 regulation. Additionally Tim-4 has been shown to be a receptor for phosphatidylserine and may play a role in engulfment of apoptotic cells. Expression is found on a subset of CD11c+ splenic cells.
Applications Reported: This 54 (RMT4-54) antibody has been reported for use in flow cytometric analysis.
Applications Tested: This 54 (RMT4-54) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Please consider the use of a viability dye like 7-AAD (cat 00-6993) when staining for Tim4.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser
The T cell immunoglobulin and mucin domain containing protein (TIM) family encodes cell surface receptors that are involved in the regulation of T helper (Th) -1 and -2 cell-mediated immunity. TIM-4, which is preferentially expressed on macrophages and dendritic cells, is the natural ligand of TIM-1, and this binding leads to T-cell expansion and cytokine production. Unlike other members of the TIM family, TIM-4 lacks a putative tyrosine phosphorylation signal sequence in its intracellular domain. The TIM-4 gene maps to a locus associated with predisposition to asthma in both mice and humans and with its connection to TIM-1-triggered Th2 responsiveness, may be considered as a candidate disease/predisposition gene for asthma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||Bimodal regulation of T cell-mediated immune responses by TIM-4.||Mizui M,Shikina T,Arase H,Suzuki K,Yasui T,Rennert PD,Kumanogoh A,Kikutani H||International immunology (20:695)||2008|
|Not Applicable||Not Cited||TIM-4 expressed on APCs induces T cell expansion and survival.||Rodriguez-Manzanet R,Meyers JH,Balasubramanian S,Slavik J,Kassam N,Dardalhon V,Greenfield EA,Anderson AC,Sobel RA,Hafler DA,Strom TB,Kuchroo VK||Journal of immunology (Baltimore, Md. : 1950) (180:4706)||2008|
|Not Applicable||Not Cited||TIM-1 and TIM-4 glycoproteins bind phosphatidylserine and mediate uptake of apoptotic cells.||Kobayashi N,Karisola P,Peña-Cruz V,Dorfman DM,Jinushi M,Umetsu SE,Butte MJ,Nagumo H,Chernova I,Zhu B,Sharpe AH,Ito S,Dranoff G,Kaplan GG,Casasnovas JM,Umetsu DT,Dekruyff RH,Freeman GJ||Immunity (27:927)||2007|