Immunofluorescent analysis of TIMP-1 was performed using 70% confluent log phase MDA-MB-231 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with TIMP-1 (102D1) Mouse Monoclonal Antibody (MS608PABX) at 2ug/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (A28175) a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Human|
|Published species reactivity||Human, Mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Recombinant human TIMP-1.|
|Storage buffer||PBS, pH 7.4|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||2 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
|Western Blot (WB)||1-3 µg/mL|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MS608PABX targets TIMP-1 in immunohistochemistry and western blot applications and shows reactivity with human samples.
The MS608PABX immunogen is recombinant human TIMP-1.
MS608PABX detects TIMP-1 which has a predicted molecular weight of approximately 21 kDa.
This gene is a member of the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. In addition to an inhibitory role against metalloproteinases, the encoded protein has a unique role among TIMP family members in its ability to directly suppress the proliferation of endothelial cells. As a result, the encoded protein may be critical to the maintenance of tissue homeostasis by suppressing the proliferation of quiescent tissues in response to angiogenic factors, and by inhibiting protease activity in tissues undergoing remodelling of the extracellular matrix.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Molecular events underlying interleukin-6 independence in a subclone of the CMA-03 multiple myeloma cell line.
MS608PABX was used in western blot to study the molecular basis for the IL-6-independent growth of a variant of the CMA-03 multiple myeloma cell line
|Verdelli D,Nobili L,Todoerti K,Mosca L,Fabris S,D'Anca M,Pellegrino E,Piva R,Inghirami G,Capelli C,Introna M,Baldini L,Chiaramonte R,Lombardi L,Neri A||Genes, chromosomes and cancer (53:154)||2014|
Hypoxia promotes metastasis in human gastric cancer by up-regulating the 67-kDa laminin receptor.
MS608PABX was used in western blot to study the role of the 67 kDa laminin receptor in the mechanism by which hypoxia promotes human gastric cancer metastasis
|Liu L,Sun L,Zhao P,Yao L,Jin H,Liang S,Wang Y,Zhang D,Pang Y,Shi Y,Chai N,Zhang H,Zhang H||Cancer science (101:1653)||2010|
The proteomes of human parotid and submandibular/sublingual gland salivas collected as the ductal secretions.
MS608PABX was used in western blot to perform a proteomic study of human saliva
|Denny P,Hagen FK,Hardt M,Liao L,Yan W,Arellanno M,Bassilian S,Bedi GS,Boontheung P,Cociorva D,Delahunty CM,Denny T,Dunsmore J,Faull KF,Gilligan J,Gonzalez-Begne M,Halgand F,Hall SC,Han X,Henson B,Hewel J,Hu S,Jeffrey S,Jiang J,Loo JA,Ogorzalek Loo RR,Malamud D,Melvin JE,Miroshnychenko O,Navazesh M,Niles R,Park SK,Prakobphol A,Ramachandran P,Richert M,Robinson S,Sondej M,Souda P,Sullivan MA,Takashima J,Than S,Wang J,Whitelegge JP,Witkowska HE,Wolinsky L,Xie Y,Xu T,Yu W,Ytterberg J,Wong DT,Yates JR,Fisher SJ||Journal of proteome research (7:1994)||2008|
Tissue inhibitor of metalloproteinase-1 protects human breast epithelial cells from extrinsic cell death: a potential oncogenic activity of tissue inhibitor of metalloproteinase-1.
MS608PABX was used in western blot to study the mechanisms by which TIMP-1 protects human breast epithelial cells from cell death
|Liu XW,Taube ME,Jung KK,Dong Z,Lee YJ,Roshy S,Sloane BF,Fridman R,Kim HR||Cancer research (65:898)||2005|
Tissue inhibitor of metalloproteinase-1 protects human breast epithelial cells against intrinsic apoptotic cell death via the focal adhesion kinase/phosphatidylinositol 3-kinase and MAPK signaling pathway.
MS608PABX was used in western blot to study the mechanism by which TIMP1 protects human breast epithelial cells against intrinsic apoptotic cell death
|Liu XW,Bernardo MM,Fridman R,Kim HR||The Journal of biological chemistry (278:40364)||2003|
The in vitro effects of dehydroepiandrosterone on human osteoarthritic chondrocytes.
MS608PABX was used in western blot to study the effects of dehydroepiandrosterone on chondrocytes isolated from human osteoarthritic cartilage
|Jo H,Park JS,Kim EM,Jung MY,Lee SH,Seong SC,Park SC,Kim HJ,Lee MC||Osteoarthritis and cartilage (11:585)||2003|
Abnormal expression of matrix metalloproteinases and tissue inhibitors of metalloproteinases in brain arteriovenous malformations.
MS608PABX was used in western blot to study the expression of MMPs and TIMPs in brain arteriovenous malformations
|Hashimoto T,Wen G,Lawton MT,Boudreau NJ,Bollen AW,Yang GY,Barbaro NM,Higashida RT,Dowd CF,Halbach VV,Young WL||Stroke (34:925)||2003|
Correlation of bacterial coinfection versus matrix metalloproteinase 9 and tissue inhibitor of metalloproteinase 1 expression in aortic aneurysm and atherosclerosis.
MS608PABX was used in immunohistochemistry - paraffin section to look for an association between Chlamydophila pneumoniae, Mycoplasma pneumoniae, matrix metalloproteinase 9, and tissue inhibitor of metalloproteinase 1 in aneurysmatic atherosclerotic lesions, and atherosclerotic nonaneurysmatic lesions
|Roggério A,Sambiase NV,Palomino SA,de Castro MA,da Silva ES,Stolf NG,de Lourdes Higuchi M||Annals of vascular surgery (27:964)||2013|
Comparative study of the immunohistochemical expression of tissue inhibitors of metalloproteinases 1 and 2 between clearly invasive carcinomas and "in situ" trophoblast invasion.
MS608PABX was used in immunohistochemistry to compare the immunohistochemical expression of TIMP1 and TIMP2 between clearly invasive carcinomas and "in situ" trophoblast invasion
|Dimo B,Ioannidis I,Karameris A,Vilaras G,Tzoumakari P,Nonni A,Patsouris E,Lazaris AC||Medical oncology (Northwood, London, England) (29:2270)||2012|
Expression of metalloproteases and their inhibitors by tumor and stromal cells in ductal carcinoma in situ of the breast and their relationship with microinvasive events.
MS608PABX was used in immunohistochemistry to study the immunohistochemical expression of MMPs and TIMPs in ductal carcinoma in situ of the breast
|González LO,González-Reyes S,Junquera S,Marín L,González L,Del Casar JM,González JM,Vizoso F||Journal of cancer research and clinical oncology (136:1313)||2010|
Immunohistochemical study of matrix metalloproteinases and their inhibitors in pure and mixed invasive and in situ ductal carcinomas of the breast.
MS608PABX was used in immunohistochemistry to study the immunohistochemical expression of MMPs and TIMPs in differerent types of breast carcinoma
|Gonzalez LO,Junquera S,del Casar JM,González L,Marín L,González-Reyes S,Andicoechea A,González-Fernández R,González JM,Pérez-Fernández R,Vizoso FJ||Human pathology (41:980)||2010|
Expression of metalloproteases and their inhibitors in different histological types of breast cancer.
MS608PABX was used in immunohistochemistry to study the expression of MMPs and TIMPs in different histological subtypes of breast cancer
|Del Casar JM,González-Reyes S,González LO,González JM,Junquera S,Bongera M,García MF,Andicoechea A,Serra C,Vizoso FJ||Journal of cancer research and clinical oncology (136:811)||2010|
Study of matrix metalloproteinases and their inhibitors in prostate cancer.
MS608PABX was used in immunohistochemistry to study the expression of MMPs and TIMPs in prostate cancer
|Escaff S,Fernández JM,González LO,Suárez A,González-Reyes S,González JM,Vizoso FJ||British journal of cancer (102:922)||2010|
Liver expression of matrix metalloproteases and their inhibitors in hepatocellular carcinoma.
MS608PABX was used in immunohistochemistry to investigate matrix metalloproteases and their inhibitors in liver and hepatocellular carcinoma
|Altadill A,Rodríguez M,González LO,Junquera S,Corte MD,González-Dieguez ML,Linares A,Barbón E,Fresno-Forcelledo M,Rodrigo L,Vizoso FJ||Digestive and liver disease : official journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver (41:740)||2009|
Immunohistochemical expression of matrix metalloproteinases in photodamaged skin by photodynamic therapy.
MS608PABX was used in immunohistochemistry to study effect of photodynamic therapy on the expression of MMPs by photo-damaged skin
|Almeida Issa MC,Piñeiro-Maceira J,Farias RE,Pureza M,Raggio Luiz R,Manela-Azulay M||The British journal of dermatology (161:647)||2009|
Comparative analysis and clinical value of the expression of metalloproteases and their inhibitors by intratumor stromal fibroblasts and those at the invasive front of breast carcinomas.
MS608PABX was used in immunohistochemistry to study the clinical significance MMP and TIMP expression by intratumor stromal and invasive front fibroblasts in breast carcinoma
|Del Casar JM,González LO,Alvarez E,Junquera S,Marín L,González L,Bongera M,Vázquez J,Vizoso FJ||Breast cancer research and treatment (116:39)||2009|
Menstrual activity of matrix metalloproteinases is decreased in endometrium regenerating after thermal ablation.
MS608PABX was used in immunohistochemistry to examine the alteration of matrix metalloproteinases during mentrual cycle
|Brun JL,Galant C,Delvaux D,Lemoine P,Henriet P,Courtoy PJ,Marbaix E||Human reproduction (Oxford, England) (24:333)||2009|
Serous and mucinous ovarian tumors express different profiles of MMP-2, -7, -9, MT1-MMP, and TIMP-1 and -2.
MS608PABX was used in immunohistochemistry to investigate the expression of MMP-2,-7,-9,MT1-MMP, and TIMP-1 and -2 in ovarian tumors
|Brun JL,Cortez A,Commo F,Uzan S,Rouzier R,Daraï E||International journal of oncology (33:1239)||2008|
A mouse to human search for plasma proteome changes associated with pancreatic tumor development.
MS608PABX was used in immunohistochemistry to identify potential serum markers of pancreatic cancer using a proteomic analysis of a genetically engineered murine model
|Faca VM,Song KS,Wang H,Zhang Q,Krasnoselsky AL,Newcomb LF,Plentz RR,Gurumurthy S,Redston MS,Pitteri SJ,Pereira-Faca SR,Ireton RC,Katayama H,Glukhova V,Phanstiel D,Brenner DE,Anderson MA,Misek D,Scholler N,Urban ND,Barnett MJ,Edelstein C,Goodman GE,Thornquist MD,McIntosh MW,DePinho RA,Bardeesy N,Hanash SM||PLoS medicine (5:null)||2008|
Androgen receptor expresion in breast cancer: relationship with clinicopathological characteristics of the tumors, prognosis, and expression of metalloproteases and their inhibitors.
MS608PABX was used in immunohistochemistry to investigate the role of androgen receptor in breast cancer and its clinicopathological manifestation
|Gonzalez LO,Corte MD,Vazquez J,Junquera S,Sanchez R,Alvarez AC,Rodriguez JC,Lamelas ML,Vizoso FJ||BMC cancer (8:null)||2008|
Gelatinase B expression as a prognostic factor in patients with stage II/III rectal carcinoma treated by postoperative adjuvant therapy.
MS608PABX was used in immunohistochemistry to investigate the prognostic value of gelatinase expression in patients with rectal carcinoma
|Unsal D,Akyurek N,Uner A,Erpolat OP,Han U,Akmansu M,Mentes BB,Dursun A||American journal of clinical oncology (31:55)||2008|
Overexpression of matrix metalloproteinases and their inhibitors in mononuclear inflammatory cells in breast cancer correlates with metastasis-relapse.
MS608PABX was used in immunohistochemistry to study the overexpression of MMPs and TIMPs in mononuclear inflammatory cells in breast cancer and the correlation with metastatic relapse
|González LO,Pidal I,Junquera S,Corte MD,Vázquez J,Rodríguez JC,Lamelas ML,Merino AM,García-Muñiz JL,Vizoso FJ||British journal of cancer (97:957)||2007|
Multinucleate giant cells release functionally unopposed matrix metalloproteinase-9 in vitro and in vivo.
MS608PABX was used in immunohistochemistry to study the secretion of MMP-9 by multinucleate giant cells in vitro and in vivo
|Zhu XW,Price NM,Gilman RH,Recarvarren S,Friedland JS||The Journal of infectious diseases (196:1076)||2007|
Matrix metalloproteinases and their inhibitors in the chamber angle of normal eyes and patients with primary open-angle glaucoma and exfoliation glaucoma.
MS608PABX was used in immunohistochemistry to study the expression of MMPs and TIMPs in the chamber angle of normal eyes and patients with primary open-angle glaucoma and exfoliation glaucoma
|Rönkkö S,Rekonen P,Kaarniranta K,Puustjärvi T,Teräsvirta M,Uusitalo H||Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fu¿r klinische und experimentelle Ophthalmologie (245:697)||2007|
Study of matrix metalloproteinases and their inhibitors in breast cancer.
MS608PABX was used in immunohistochemistry to study the role of MMPs and TIMPs in breast cancer
|Vizoso FJ,González LO,Corte MD,Rodríguez JC,Vázquez J,Lamelas ML,Junquera S,Merino AM,García-Muñiz JL||British journal of cancer (96:903)||2007|
Usefulness of MMP-9/TIMP-1 in predicting tumor recurrence in patients undergoing curative surgical resection for gastric carcinoma.
MS608PABX was used in immunohistochemistry to value of tumor MMP9 and TIMP1 levels for predicting tumor recurrence in patients undergoing curative resection for gastric carcinoma
|Seo YS,Park JJ,Kim JH,Kim JY,Yeon JE,Kim JS,Byun KS,Bak YT||Digestive diseases and sciences (52:753)||2007|
Matrix metalloproteinase-9 expression correlated with tumor response in patients with locally advanced rectal cancer undergoing preoperative chemoradiotherapy.
MS608PABX was used in immunohistochemistry to examine the prognostic significance of matrix metalloproteinase-9 for locally advanced rectal cancer undergoing preoperative chemoradiotherapy
|Unsal Kilic D,Uner A,Akyurek N,Erpolat P,Dursun A,Pak Y||International journal of radiation oncology, biology, physics (67:196)||2007|
In vitro modeling of human pancreatic duct epithelial cell transformation defines gene expression changes induced by K-ras oncogenic activation in pancreatic carcinogenesis.
MS608PABX was used in immunohistochemistry to investigate the role of K-ras oncogenic activation in pancreatic carcinogenesis
|Qian J,Niu J,Li M,Chiao PJ,Tsao MS||Cancer research (65:5045)||2005|
Upregulation of the tissue inhibitor of metalloproteinase-1 protein is associated with progression of human non-small-cell lung cancer.
MS608PABX was used in immunohistochemistry to investigate the association of TIMP-1 upregulation with the progression of human non-small-cell lung cancer
|Aljada IS,Ramnath N,Donohue K,Harvey S,Brooks JJ,Wiseman SM,Khoury T,Loewen G,Slocum HK,Anderson TM,Bepler G,Tan D||Journal of clinical oncology : official journal of the American Society of Clinical Oncology (22:3218)||2004|
Unopposed matrix metalloproteinase-9 expression in human tuberculous granuloma and the role of TNF-alpha-dependent monocyte networks.
MS608PABX was used in immunohistochemistry to investigate the roles of metalloproteinase-9 and TNF-alpha-dependent monocyte networks in human tuberculous granulom
|Price NM,Gilman RH,Uddin J,Recavarren S,Friedland JS||Journal of immunology (Baltimore, Md. : 1950) (171:5579)||2003|
Imbalance between expression of matrix metalloproteinase-9 and tissue inhibitor of metalloproteinase-1 in invasiveness and metastasis of human gastric carcinoma.
MS608PABX was used in immunohistochemistry to study the role of MMP-9 and TIMP-1 imbalance in metastasis and poor prognosis in gastric carcinoma
|Zhang S,Li L,Lin JY,Lin H||World journal of gastroenterology (9:899)||2003|
Systemic arterial expression of matrix metalloproteinases 2 and 9 in acute Kawasaki disease.
MS608PABX was used in immunohistochemistry to study the expression of MMP2, MMP9, TIMP1 and TIMP2 in arteries from patients with fatal acute Kawasaki's disease
|Gavin PJ,Crawford SE,Shulman ST,Garcia FL,Rowley AH||Arteriosclerosis, thrombosis, and vascular biology (23:576)||2003|
Tissue inhibitor of matrix metalloproteinase-1 overexpression in M1 myeloblasts impairs IL-6-induced differentiation.
MS608PABX was used in blocking or activating experiment to study the role of TIMP1 overexpression in impairing IL-6-induced differentiation in M1 myeloblasts
|Haviernik P,Lahoda C,Bradley HL,Hawley TS,Ramezani A,Hawley RG,Stetler-Stevenson M,Stetler-Stevenson WG,Bunting KD||Oncogene (23:9212)||2004|