|Tested species reactivity||Human|
|Published species reactivity||Rat, Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Native human pro-MMP-2/TIMP-2 complex purified from A2058 culture medium followed by purified human rTIMP-2|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunoprecipitation (IP)||2 µg/ml|
|Western Blot (WB)||1-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-13600 targets TIMP-2 in IP and WB applications and shows reactivity with Human samples.
The MA5-13600 immunogen is native human pro-MMP-2/TIMP-2 complex purified from A2058 culture medium followed by purified human rTIMP-2.
Tissue inhibitor of metalloproteinases-1 (TIMP-1) and Tissue inhibitor of metalloproteinases-2 (TIMP-2) have similar properties, specifically in inhibiting enzymes of matrix metalloproteinase family, and are thought to be of great importance in the maintenance of connective tissue integrity. TIMP-2 shows the highest binding affinity to both the latent (pro) and active forms of 72kDa Type IV collagenase (also known as MMP-2 or gelatinase A). It also has affinity for the active form of 92kDa Type IV collagenase (also known as MMP-9 or gelatinase B). TIMP-2 inhibits the proteolytic invasiveness of tumor cells and normal placental trophoblast cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Effects of cigarette smoke condensate on oral squamous cell carcinoma cells.
MA5-13600 was used in western blot to study the effect of cigarette smoke condensate exposure of oral squamous carcinoma cells on the expression of MMP2 and MMP9 and their ability to degrade collagen
|Allam E,Zhang W,Al-Shibani N,Sun J,Labban N,Song F,Windsor LJ||Archives of oral biology (56:1154)||2011|
Cigarette smoke condensate affects the collagen-degrading ability of human gingival fibroblasts.
MA5-13600 was used in western blot to study the effect of cigarette smoke condensate on the collagen-degrading ability of human gingival fibroblasts
|Zhang W,Song F,Windsor LJ||Journal of periodontal research (44:704)||2009|
Modulation of extracellular matrix components by metalloproteinases and their tissue inhibitors during degeneration and regeneration of rat sural nerve.
MA5-13600 was used in immunohistochemistry and western blot to study the role of MMPs and TIMPs in remodelleing the extracellular matrix during degeneration and regeneration of rat sural nerve
|Gantus MA,Nasciutti LE,Cruz CM,Persechini PM,Martinez AM||Brain research (1122:36)||2006|