Western blot analysis was performed on tissue extracts (30 ug lysate) of Mouse colon (Lane 1), and Mouse spleen (Lane 2). The blots were probed with Anti-TIMP2 Mouse Monoclonal Antibody (Product# MA513603, 1-2 ug/mL) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.4 ug/ml, 1:2500 dilution). A ~ 24 kDa band corresponding to TIMP2 was observed across tissues tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Native human pro-MMP-2/TIMP-2 complex purified from A2058 culture medium followed by purified human rTIMP-2|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunoprecipitation (IP)||2µg/mg protein lysate|
|Western Blot (WB)||1-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 2 publications below|
MA5-13603 targets TIMP-2 in Immunoprecipitation and Western blot applications and shows reactivity with Human samples.
The MA5-13603 immunogen is native human pro-MMP-2/TIMP-2 complex purified from A2058 culture medium followed by purified human rTIMP-2.
Tissue inhibitor of metalloproteinases-1 (TIMP-1) and Tissue inhibitor of metalloproteinases-2 (TIMP-2) have similar properties, specifically in inhibiting enzymes of matrix metalloproteinase family, and are thought to be of great importance in the maintenance of connective tissue integrity. TIMP-2 shows the highest binding affinity to both the latent (pro) and active forms of 72kDa Type IV collagenase (also known as MMP-2 or gelatinase A). It also has affinity for the active form of 92kDa Type IV collagenase (also known as MMP-9 or gelatinase B). TIMP-2 inhibits the proteolytic invasiveness of tumor cells and normal placental trophoblast cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Effects of cigarette smoke condensate on oral squamous cell carcinoma cells.
MA5-13603 was used in western blot to study the effect of cigarette smoke condensate exposure of oral squamous carcinoma cells on the expression of MMP2 and MMP9 and their ability to degrade collagen
|Allam E,Zhang W,Al-Shibani N,Sun J,Labban N,Song F,Windsor LJ||Archives of oral biology (56:1154)||2011|
Cigarette smoke condensate affects the collagen-degrading ability of human gingival fibroblasts.
MA5-13603 was used in western blot to study the effect of cigarette smoke condensate on the collagen-degrading ability of human gingival fibroblasts
|Zhang W,Song F,Windsor LJ||Journal of periodontal research (44:704)||2009|