Invitrogen

TIMP1 Monoclonal Antibody (102D1)

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated. View Details

35 References

View all (20) TIMP1 antibodies

Datasheet

Questions & Answers

Datasheet

Questions & Answers

Product Image Gallery

TIMP1 Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of TIMP-1 was performed using 70% confluent log phase MDA-MB-231 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with TIMP-1 (102D1) Mouse Monoclonal Antibody (Product # MS608PABX) at 1:250 dilution in0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.

TIMP1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of TIMP-1 (102D1) showing staining in the cytoplasm of paraffin-embedded human prostate tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a TIMP-1 Antibody (102D1) Mouse Monoclonal Antibody (Product # MS608PABX) diluted in 3% BSA-PBS at a dilution of 1:100 for 1 hour at 37°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

TIMP1 Antibody in Western Blot (WB) — Western blot analysis was performed on (Fig 1) whole cell extracts (30 µg lysate) of HeLa (Lane 1), HT-29 (Lane 2), SK-OV-3 (Lane 3), MCF7 (Lane 4), PC-3 (Lane 5), K-562 (Lane 6) and HCT 116 (Lane 7). Likewise Western blot analysis was performed on (Fig 2) 10 uL HeLa conditioned media (Lane 1) and 10 uL MDA-MB-231 conditioned media (Lane 2). The blots were probed with Anti-TIMP-1 Receptor Mouse Monoclonal Antibody (Product # MS608PABX, 1:500 dilution) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.4 µg/mL, 1:2500 dilution). A 23 kDa band corresponding to TIMP-1 was observed across the cell lines and conditioned media tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel (Product # NP0342BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody using iBind™ Flex Western Starter Kit (Product # SLF2000S). Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

TIMP1 Antibody in Relative expression — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lysates tested owing to their inherent genetic constitution. Relative expression of TIMP1 was observed in MDA-MB-231 as compared to Daudi using Anti-TIMP1 Monoclonal Antibody (102D1) (Product # MS608PABX) in Western Blot. {RE}

Product Details

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC)

1:250

Immunofluorescence (IF)

1:250

Immunohistochemistry (Paraffin) (IHC (P))

1:100

View 1 publication 1 publication

Western Blot (WB)

1:500

View 7 publications 7 publications

Immunohistochemistry (IHC)

View 26 publications 26 publications

Neutralization (Neu)

View 1 publication 1 publication

Product Specifications

Species

Human

Published species

Human, Mouse

Expression System

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

102D1

Immunogen

Recombinant human TIMP-1.

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4

Contains

no preservative

Storage conditions

-20°C

RRID

AB_417075

Product Specific Information

MS608PABX targets TIMP-1 in immunohistochemistry and western blot applications and shows reactivity with human samples.

The MS608PABX immunogen is recombinant human TIMP-1.

MS608PABX detects TIMP-1 which has a predicted molecular weight of approximately 21 kDa.

Target Information

TIMP1 (metalloproteinase inhibitor 1) is a metalloproteinase inhibitor that functions by forms one-to-one complexes with target metallproteinases, such as collagenases, and irreversible inactivates them by binding to their catalytic zinc cofactor. TIMP1 acts on MMP1, MMP2, MMP3, MMP7, MMP8, MMP9, MMP10, MMP11, MMP12, MMP14, and MMP16. It also functions as a growth factor that regulates cell differenation, migration, and cell death. TIMP1 activates cellular signaling cascades via CD63 and ITGB1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Collagenase inhibitor; EPA; erythroid potentiating activity; Erythroid-potentiating activity; Fibroblast collagenase inhibitor; FLJ90373; Metalloproteinase inhibitor; Metalloproteinase inhibitor 1; MMP inhibitor; RP1-230G1.3; TIMP 1; TIMP-1; Tissue inhibitor of metalloproteinases 1

Gene Aliases: CLGI; EPA; EPO; HCI; TIMP; TIMP-1; TIMP1

UniProt ID: (Human) P01033

Entrez Gene ID: (Human) 7076

Function(s)