Note: You clicked on an external link, which has been disabled in order to keep your shopping session open.
Western blot analysis using the anti-TIP5 crude serum. Nuclear extracts (150 µg) from NIH3T3 or HeLa cells were analyzed by western blot using the anti-TIP5 crude serum (Cat. no. 49-1037), diluted 1:1,000 in PBS containing 5% milk powder and 0.1% Tween-20. The molecular weight marker is shown on the left, the location of the protein of interest is indicated on the right.
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant protein TIP5|
|Storage buffer||whole serum|
|Contains||<0.1% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ChIP assay (ChIP)||5 ul|
|Western Blot (WB)||1:1,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
TIP 5 is the large subunit of the nucleolar remodeling complex NoRC. NoRC causes the repression of ribosomal gene transcription. It was demonstrated that histone deacetylation is involved in this repression and that TIP5 is associated with the histone deacetylase HDAC1 and mediates the deacetylation of histones in the vicinity of the rDNA promoter. The interaction of TIP5 and HDAC1, which is necessary for transcriptional repression, is established by the C-terminal PHD finger and bromodomain.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Baz2a; bromodomain adjacent to zinc finger domain protein 2A; bromodomain adjacent to zinc finger domain, 2A; Kiaa0314; Tip5; transcription termination factor I-interacting protein 5; TTF-I interacting peptide 5; TTF-I-interacting protein 5
AA415431; BAZ2A; C030005G16Rik; C78388; KIAA0314; mKIAA0314; TIP5; WALp3