NeoBiotechnologies

TLE1 Monoclonal Antibody (TLE1, 2051)

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TLE1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry (Paraffin) analysis performed in formalin fixed, paraffin-embedded human endometrial carcinoma using TLE1 Monoclonal Antibody (Product # 7088-MSM1-P1ABX).

TLE1 Antibody in Western Blot (WB) — Western Blot analysis of human HeLa cell lysate using TLE1 Monoclonal Antibody (Product # 7088-MSM1-P1ABX).

TLE1 Antibody in Peptide array (ARRAY) — Analysis of Protein Array containing more than 19,000 full-length human proteins TLE1 Monoclonal Antibody (Product # 7088-MSM1-P1ABX). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

TLE1 Antibody — SDS-PAGE Analyis Purified TLE1 Monoclonal Antibody (Product # 7088-MSM1-P1ABX). Confirmation of Purity and Integrity of Antibody.

Product Details

7088-MSM1-P1ABX

Applications	Tested Dilution	Publications
Western Blot (WB)	1-2 µg/mL	-
Immunohistochemistry (Paraffin) (IHC (P))	1-2 µg/mL	-
Peptide Array (Array)	Assay-dependent	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Mouse / IgG2a, kappa
Class	Monoclonal
Type	Antibody
Clone	TLE1, 2051
Immunogen	Recombinant human TLE1 fragment (aa 175-338) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Protein A
Storage buffer	PBS, pH 7.4
Contains	no preservative
Storage conditions	-20°C or -80°C if preferred

Product Specific Information

Immunohistochemistry (PFA fixed): incubate antibody for 30 min RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 degrees C followed by cooling at RT for 20 minutes.

Target Information

Transcriptional corepressor that binds to a number of transcription factors. Inhibits NF-kappa-B-regulated gene expression. Inhibits the transcriptional activation mediated by FOXA2, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Unusual function as coactivator for ESRRG.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: E(Sp1) homolog; Enhancer of split groucho-like protein 1; ESG1; RP11-203D19.1; TLE 1; transducin-like enhancer of split 1 (E(sp1) homolog, Drosophila); Transducin-like enhancer protein 1

Gene Aliases: ESG; ESG1; GRG1; TLE1

UniProt ID: (Human) Q04724

Entrez Gene ID: (Human) 7088

Molecular Function: transcription cofactor

Function(s)

negative regulation of transcription from RNA polymerase II promoter transcription, DNA-templated signal transduction multicellular organism development organ morphogenesis positive regulation of gene expression Wnt signaling pathway negative regulation of Wnt signaling pathway negative regulation of I-kappaB kinase/NF-kappaB signaling negative regulation of transcription, DNA-templated beta-catenin-TCF complex assembly negative regulation of anoikis

Process(es)

RNA polymerase II transcription corepressor activity protein binding transcription factor binding identical protein binding

TLE1 Monoclonal Antibody (TLE1, 2051)

Product Details

7088-MSM1-P1ABX

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Peptide Array (Array)

Species Reactivity

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Product Specific Information

Target Information

References (0)

Bioinformatics

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