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Flow cytometry of Cell surface of TLR2 in 10^6 PBMCs using 1 ug of TLR2/CD282 monoclonal antibody (Product # MA5-16200). Shaded histogram represents cells without antibody; green represents isotype control; red represents TLR2/CD282 monoclonal antibody (Product # MA5-16200). Goat anti-mouse IgG PE secondary was used in this test.
|Tested species reactivity||Dog, Human|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||CHO cells transfected with human TLR2 cDNA|
|Storage buffer||PBS with 0.05% BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Blocking Assay (BLOCK)||Assay-Dependent|
|Flow Cytometry (Flow)||1-2 µg/10^6 cells|
|Functional Assay (FN)||Assay-Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:500|
|Immunoprecipitation (IP)||2-5 µg|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Suggested positive control: antigen standard for TLR2 (transient overexpression lysate), PBMCs, Ramos.
The Toll-like receptors (TLRs) in mammals comprise a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and an IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1). The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition (2). Ten human homologs of TLRs (TLR1-10) have been described (3). TLR2 is differentially expressed in human cells. TLR2 is expressed in tonsils, lymph nodes, and appendices, activated B-cells in germinal centers. CD14+ monocytes expressed the highest level of TLR2 followed by CD15+ granulocytes, and CD19+ B-cells, CD3+ T-cells, and CD56+ NK cells did not express TLR2. The expression of TLR2 in different cell types is regulated by different immune response modifiers. For example, LPS, GM-CSF, IL-1, and IL-10 up regulates TLR2 whereas IL-4, IFN-gamma, and TNF down regulate TLR2 expression in monocytes (4).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
TIL4; Toll-like Receptor 2; toll/interleukin 1 receptor-like 4; toll/interleukin-1 receptor-like protein 4
CD282; TIL4; TLR2