Flow cytometry of Cell surface of TLR2 in 10^6 PBMCs using 1 ug of TLR2/CD282 monoclonal antibody (Product # MA5-16200). Shaded histogram represents cells without antibody; green represents isotype control; red represents TLR2/CD282 monoclonal antibody (Product # MA5-16200). Goat anti-mouse IgG PE secondary was used in this test.
|Tested species reactivity||Dog, Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||CHO cells transfected with human TLR2 cDNA|
|Storage buffer||PBS with 0.05% BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1-2 µg/10^6 cells|
|Functional Assay (FN)||Assay-Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:500|
|Immunoprecipitation (IP)||2-5 µg|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Blocking Assay (BLOCK)||See 1 publications below|
Suggested positive control: antigen standard for TLR2 (transient overexpression lysate), PBMCs, Ramos.
The Toll-like receptors (TLRs) in mammals comprise a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and an IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1). The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition (2). Ten human homologs of TLRs (TLR1-10) have been described (3). TLR2 is differentially expressed in human cells. TLR2 is expressed in tonsils, lymph nodes, and appendices, activated B-cells in germinal centers. CD14+ monocytes expressed the highest level of TLR2 followed by CD15+ granulocytes, and CD19+ B-cells, CD3+ T-cells, and CD56+ NK cells did not express TLR2. The expression of TLR2 in different cell types is regulated by different immune response modifiers. For example, LPS, GM-CSF, IL-1, and IL-10 up regulates TLR2 whereas IL-4, IFN-gamma, and TNF down regulate TLR2 expression in monocytes (4).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Differential neutrophil responses to bacterial stimuli: Streptococcal strains are potent inducers of heparin-binding protein and resistin-release.
MA5-16200 was used in blocking or activating experiment to examine neutrophil responses such as heparin-binding protein and resistin production during sepsis
|Snäll J,Linnér A,Uhlmann J,Siemens N,Ibold H,Janos M,Linder A,Kreikemeyer B,Herwald H,Johansson L,Norrby-Teglund A||Scientific reports (6:null)||2016|