Immunohistochemical analysis of TLR3/CD283 in human gut lumen (A, longitudinal section, transverse region) using a TLR3/CD283 monoclonal antibody (Product # MA1-25649) at a dilution of 10 ug/ml and mouse spleen tissue using an isotype control (B) and MA1-256489 (C) at 5 mg/ml.
|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Synthetic peptide, conjugated to KLH, corresponding to a portion of human TLR3.|
|Storage buffer||0.2% gelatin|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||2-5 µg for 10^6 cells|
|Immunoprecipitation (IP)||2µg for 10^6 cells|
|Western Blot (WB)||1-3 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-25649 detects TLR3 from human samples.
MA1-25649 has been successfully used in immunoprecipitation, flow cytometry and Western blot procedures.
The MA1-25649 immunogen is a synthetic peptide, conjugated to KLH, corresponding to a portion of human TLR3.
The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This receptor is most abundantly expressed in placenta and pancreas, and is restricted to the dendritic subpopulation of the leukocytes. It recognizes dsRNA associated with viral infection, and induces the activation of NF-kappaB and the production of type I interferons. It may thus play a role in host defense against viruses. Use of alternative polyadenylation sites to generate different length transcripts has been noted for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.