|Flow Cytometry (Flow)||2-5 µg for 10^6 cells|
|Immunoprecipitation (IP)||2µg for 10^6 cells|
|Western Blot (WB)||1-3 µg/ml|
|Tested Species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Synthetic peptide, conjugated to KLH, corresponding to a portion of human TLR3.|
|Storage buffer||0.2% gelatin|
|Contains||0.05% sodium azide|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
MA1-25649 detects TLR3 from human samples.
MA1-25649 has been successfully used in immunoprecipitation, flow cytometry and Western blot procedures.
The MA1-25649 immunogen is a synthetic peptide, conjugated to KLH, corresponding to a portion of human TLR3.
The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This receptor is most abundantly expressed in placenta and pancreas, and is restricted to the dendritic subpopulation of the leukocytes. It recognizes dsRNA associated with viral infection, and induces the activation of NF-kappaB and the production of type I interferons. It may thus play a role in host defense against viruses. Use of alternative polyadenylation sites to generate different length transcripts has been noted for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CD283; IIAE2; Toll-like Receptor 3
Gene Aliases: CD283; IIAE2; TLR3
UniProt ID: (Human) O15455
Entrez Gene ID: (Human) 7098
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