|Tested species reactivity||Human, Mouse|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A peptide corresponding to 15 amino acids near the amino terminus of human TLR3.|
|Contains||0.02% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||10 µg/ml|
|Immunofluorescence (IF)||2 ug/ml|
|Western Blot (WB)||0.5-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
A suggested positive control is K562 cell lysate.
PA5-20184 can be used with blocking peptide PEP-0314.
Toll-like receptors (TLRs) are evolutionarily conserved pattern-recognition molecules resembling the toll proteins that mediate antimicrobial responses in Drosophila. These proteins recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. The TLRs act through adaptor molecules such as MyD88 and TIRAP to activate various kinases and transcription factors so the organism can respond to potential infection. TLR3 is known to recognize viral double-stranded (ds) RNA, a molecular pattern associated with viral infection. Recently it has been shown to recognize viruses such as Influenza A and West Nile Virus and can mediate entry of at least West Nile Virus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
AP1S3 mutations are associated with pustular psoriasis and impaired Toll-like receptor 3 trafficking.
PA5-20184 was used in western blot to study the role of TLR3 vesicular trafficking defects in the mechanism by which AP1S3 mutations promote pustular psoriasis
|Setta-Kaffetzi N,Simpson MA,Navarini AA,Patel VM,Lu HC,Allen MH,Duckworth M,Bachelez H,Burden AD,Choon SE,Griffiths CE,Kirby B,Kolios A,Seyger MM,Prins C,Smahi A,Trembath RC,Fraternali F,Smith CH,Barker JN,Capon F||American journal of human genetics (94:790)||2014|