FACS analysis of cell surface TLR4 in human monocyte-derived dendritic cells using a TLR4/CD284 monoclonal antibody (Product # MA5-16219) at 1:200 (2.5 ug). Black and green open histograms represent unstained cells and negative control (human CD14 FITC) respectively. Blue open histogram is TLR4/CD284 monoclonal antibody (Product # MA5-16219).
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Mouse / IgG2b, kappa|
|Immunogen||Synthetic peptide (aa 125-140) of TLR4/CD284 protein.|
|Storage buffer||50mM sodium borate|
|Contains||0.05% sodium azide|
|Storage Conditions||4° C, store in dark|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||2-5 µg/10^6 cells|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Suggested positive control: Ramos or ThP1.
The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1). The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition (2). Ten human homologs of TLRs (TLR1-10) have been described (3). Among this family of receptors, TLR2 and TLR4 have been most studied. These studies have suggested that TLR2 and TLR4 may serve as potential main mediators of LPS signaling (4,5). The TLR4 cDNA codes for a protein consisting of 799 amino acids with approximate molecular weight of 88 kDa (6).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.