|Tested species reactivity||Human, Non-human primate|
|Published species reactivity||Mouse|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Ba/F3 cell line expressing TLR4.|
|Storage buffer||PBS with 1% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||10 µl for 10^6 cells in 100 µl|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Blocking Assay (BLOCK)||See 1 publications below|
MA1-20236 detects TLR4 in human and non-human primate samples.
MA1-20236 has been successfully used in FACS procedures.
The MA1-20236 immunogen corresponds to Ba/F3 cell line expressing TLR4.
TLR4 is a member of the Toll like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. This receptor is most abundantly expressed in placenta, and in myelomonocytic subpopulation of the leukocytes. It has been implicated in signal transduction events induced by lipopolysaccharide (LPS) found in most gram-negative bacteria.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Dysfunctional B-cell activation in cirrhosis resulting from hepatitis C infection associated with disappearance of CD27-positive B-cell population.
MA1-20236 was used in blocking or activating experiment to study the role of hepatitis C infection in the B-cell dysfunction in cirrhosis
|Doi H,Iyer TK,Carpenter E,Li H,Chang KM,Vonderheide RH,Kaplan DE||Hepatology (Baltimore, Md.) (55:709)||2012|