|Tested species reactivity||Dog, Guinea pig, Human, Non-human primate, Pig|
|Published species reactivity||Not Applicable|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Ba/F3 cell line expressing TLR4 (CD284)|
|Contains||0.09% sodium azide|
|Storage Conditions||4°C or -20°C if preferred|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:10 -1:25|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Flow Cytometry (Flow)||See 1 publications below|
For FACS analysis, use 10ul of the suggested working dilution to label 1x10^6 cells in 100ul.
TLR4 is a member of the Toll like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. This receptor is most abundantly expressed in placenta, and in myelomonocytic subpopulation of the leukocytes. It has been implicated in signal transduction events induced by lipopolysaccharide (LPS) found in most gram-negative bacteria.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Human CD14 is an efficient target for recombinant immunoglobulin vaccine constructs that deliver T cell epitopes.
MA5-16719 was used in flow cytometry to assess targeting CD14 using recombinant Ig-based vaccines in order to elicit human T cell responses
|Tunheim G,Schjetne KW,Fredriksen AB,Sandlie I,Bogen B||Journal of leukocyte biology (77:303)||2005|