Immunofluorescence analysis of TLR5 was performed using 70% confluent log phase RAW 264.7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with TLR5 Rabbit Polyclonal Antibody (Product # 36-3900) at 2 µg/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conj µgate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing localization in the membrane. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Mouse, Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Raised against the C-term of human TLR5.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||2 µg/ml|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene product is expressed in myelomonocytic cells, and recognizes bacterial flagellin, a principal component of bacterial flagella and a virulence factor. The activation of this receptor mobilizes the nuclear factor NF-kappaB and stimulates tumour necrosis factor-alpha production.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
A modified controlled cortical impact technique to model mild traumatic brain injury mechanics in mice.
36-3900 was used in immunohistochemistry - frozen section to evaluate a cortical impact technique for brain trauma studies
|Chen Y,Mao H,Yang KH,Abel T,Meaney DF||Frontiers in neurology (5:null)||2014|
Pseudomonas aeruginosa-induced apoptosis in airway epithelial cells is mediated by gap junctional communication in a JNK-dependent manner.
36-3900 was used in immunohistochemistry to investigate the role of gap junctional communication in Pseudomonas aeruginosa-induced apoptosis in airway epithelial cells
|Losa D,Köhler T,Bellec J,Dudez T,Crespin S,Bacchetta M,Boulanger P,Hong SS,Morel S,Nguyen TH,van Delden C,Chanson M||Journal of immunology (Baltimore, Md. : 1950) (192:4804)||2014|
||Toll-like receptors TLR4, TLR5 and TLR9 on gastric carcinoma cells: an implication for interaction with Helicobacter pylori.||Schmausser B,Andrulis M,Endrich S,Müller-Hermelink HK,Eck M||International journal of medical microbiology : IJMM (295:179)||2005|
|Human||Not Cited||Toll-like receptors TLR4, TLR5 and TLR9 on gastric carcinoma cells: an implication for interaction with Helicobacter pylori.||Schmausser B,Andrulis M,Endrich S,Müller-Hermelink HK,Eck M||International journal of medical microbiology : IJMM (295:179)||2005|