|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Mouse, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A peptide corresponding to 15 amino acids near the center of human TLR9.|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||2 ug/ml|
|Immunofluorescence (IF)||20 ug/ml|
|Western Blot (WB)||0.5-2 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
A suggested positive control is Jurkat cell lysate.
PA5-20203 can be used with blocking peptide PEP-0346.
Toll-like receptors (TLRs) are evolutionarily conserved pattern-recognition molecules resembling the toll proteins that mediate antimicrobial responses in Drosophila. These proteins recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. TLR9 forms a subfamily along with TLR7 and TLR8 that recognize viral RNA and CpG DNA sequences and are localized in intracellular acidic compartments such as the phagolysosome. Unlike other TLRs which act through adaptor molecules such as TOLLIP, TIRAP, TRIF, and MyD88 to activate various kinases and transcription factors to respond to potential infection, TLR9 is strictly dependent on MyD88.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Munc13-4 Is a Rab11-binding Protein That Regulates Rab11-positive Vesicle Trafficking and Docking at the Plasma Membrane.
PA5-20203 was used in western blot to study regulation of Rab11-positive vesicle trafficking and docking at the plasma membrane by Munc13-4, a Rab11-binding protein
|Johnson JL,He J,Ramadass M,Pestonjamasp K,Kiosses WB,Zhang J,Catz SD||The Journal of biological chemistry (291:3423)||2016|
Munc13-4 interacts with syntaxin 7 and regulates late endosomal maturation, endosomal signaling, and TLR9-initiated cellular responses.
PA5-20203 was used in immunocytochemistry to investigate a mechanism of late endosomal maturation
|He J,Johnson JL,Monfregola J,Ramadass M,Pestonjamasp K,Napolitano G,Zhang J,Catz SD||Molecular biology of the cell (27:572)||2016|