|Tested species reactivity||Chemical, Tag|
|Host / Isotype||Mouse / IgG2b|
|Storage buffer||PBS with 4% BSA|
|Tested Applications||Dilution *|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Product # 90075 binds to immobilized TMT surface.
Product # 90075 has been succesfully used in immunoprecipitation and Western blot applications.
Tandem Mass Tag (TMT) reagents enable concurrent identification and multiplexed quantitation of proteins in different samples using tandem mass spectrometry (MS). Isobaric TMT reagents share identical strucures, including a peptide-reactive labeling group, a spacer arm and a MS/MS reporter. During the MS/MS analysis, each isobaric tag produces a unique reporter ion that makes quantitation possible. Amine-reactive NHS-ester isotopic duplex or six-plex isobaric TMT tags covalently react with amino termini and lysine residues of peptides and proteins. Cysteine-reactive Tandem Mass Tag (cysTMT) reagents react with reduced thiols of cysteine-containing peptides.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.