|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide to the C-terminal region of mouse TNF receptor II protein.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50|
|Immunoprecipitation (IP)||2 µg/ml|
|Western Blot (WB)||1-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 1 publications below|
PA1-21148 detects TNF receptor II in human, mouse and rat samples.
PA1-21148 has been successfully used in immunohistochemistry (paraffin), Immunoprecipitation and Western blot procedures.
The PA1-21148 immunogen is a synthetic peptide to the C-terminal region of mouse TNF receptor II protein.
Tumor Necrosis Factor (TNF) is a cytokine whose function is mediated through two distinct cell surface receptors (TNFR 1 and TNFR 2) that are included in the TNF receptor superfamily along with FAS antigen and CD40. Tumor necrosis factor receptor 1 and 2 (TNFR 1 and TNFR 2) are 55 and 75 kDa members of a family cell surface molecules including nerve growth factor receptor, Fas/Apo1, CD30, Ox40, and 4-1BB, which are characterized by cysteine rich motifs in the extracellular domain.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Adipocytes as immune cells: differential expression of TWEAK, BAFF, and APRIL and their receptors (Fn14, BAFF-R, TACI, and BCMA) at different stages of normal and pathological adipose tissue development.
PA1-21148 was used in immunohistochemistry to investigate the expression level of multiple genes in adipose tissues
|Alexaki VI,Notas G,Pelekanou V,Kampa M,Valkanou M,Theodoropoulos P,Stathopoulos EN,Tsapis A,Castanas E||Journal of immunology (Baltimore, Md. : 1950) (183:5948)||2009|