|Immunohistochemistry (Paraffin) (IHC (P))||1:50|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from C-terminus of mouse TNF-R2.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage conditions||4° C, do not freeze|
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is liver carcinoma and thymus tissue.
Tumor necrosis factor (TNF) is a pleiotropic cytokine whose function is mediated through two distinct cell surface receptors. These receptors, designated TNF-R1 and TNF-R2, are 55 kDa and 75kDa, respectively, and are expressed on most cell types. Signaling through TNF-R2 is confined to cells of the immune system.
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Protein Aliases: CD120b; p75; p75 TNF receptor; p75TNFR; p80 TNF-alpha receptor; soluble TNFR1B variant 1; TBPII; TNF-R-II; TNF-R2; TNF-R75; TNF-RII; TNFBR; TNFR1B; TNFR2; TNFR80; tumor necrosis factor beta receptor; tumor necrosis factor binding protein 2; Tumor necrosis factor receptor 2; Tumor necrosis factor receptor superfamily member 1B; tumor necrosis factor receptor superfamily, member 1B; Tumor necrosis factor receptor type II
Gene Aliases: CD120b; p75; p75TNFR; TBPII; TNF-R-II; TNF-R75; TNFBR; TNFR1B; TNFR2; TNFR80; TNFRSF1B
UniProt ID: (Human) P20333
Entrez Gene ID: (Human) 7133
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