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|Tested species reactivity||Mouse|
|Published species reactivity||Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant protein corresponding to amino acids 80–235 of mouse tumor necrosis factor alpha|
|Contains||0.09% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Western Blot (WB)||0.1-2 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
This antibody is predicted to react with rat based on sequence homology.
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale, and purified with Protein A.
ABfinity™ monoclonal antibodies resemble rabbit monoclonals isolated from serum or produced by hybridomas, but demonstrate greater specificity and sensitivity. Because ABfinity™ recombinant antibodies are derived from cloned DNA sequences of the heavy and light antibody chains, they are not susceptible to cell-line drift or lot-to-lot variation, thus allowing for peak specificity and performance.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Tumor necrosis factor-alpha is a pleiotropic inflammatory cytokine that stimulates the acute phase reaction. The primary role of TNF is in the regulation of immune cells. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF-alpha is a trimeric protein encoded within the major histocompatibility complex.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Retinol dehydrogenase 13 protects the mouse retina from acute light damage.
701135 was used in western blot to study the protective role of retinol dehydrogenase 13 against light-induced retinopathy in the mouse
|Wang H,Cui X,Gu Q,Chen Y,Zhou J,Kuang Y,Wang Z,Xu X||Molecular vision (18:1021)||2012|
cachectin; TNF alpha; TNF-a; tumor necrosis factor; tumor necrosis factor alpha; tumor necrosis factor ligand superfamily member 2; tumor necrosis factor-alpha
DIF; Tnf; TNF-a; TNF-alpha; Tnfa; TNFalpha; Tnfsf1a; TNFSF2