|Tested species reactivity||Human|
|Published species reactivity||Human, Not Applicable|
|Host / Isotype||Mouse / IgG1/IgG2a|
|Clone||68B2B3 and 68B6A3|
|Immunogen||Purified recombinant human TNF-alpha.|
|Storage buffer||PBS, pH 7.5|
|Contains||0.05% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This monoclonal mixture is recommended as a capture antibody in Sandwich ELISA applications.
This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Apoptotic responses in squamous carcinoma and epithelial cells to small-molecule toll-like receptor agonists evaluated with automated cytometry.
AHC3712 was used in ELISA to develop an 96-well assay to quantitate DNA fragmentation in cells
|Inglefield JR,Larson CJ,Gibson SJ,Lebrec H,Miller RL||Journal of biomolecular screening (11:575)||2006|
|Not Applicable||8 µg/ml||
The effect of aging and inflammation on heat shock protein 27 in human monocytes and lymphocytes.
AHC3712 was used in ELISA to examine the effect of age and inflammation on the induction of Hsp27 in human peripheral blood mononuclear cells
|Njemini R,Lambert M,Demanet C,Mets T||Experimental gerontology (41:312)||2006|
|Not Applicable||Not Cited||
Infliximab induces apoptosis of monocytes and T lymphocytes in a human-mouse chimeric model.
AHC3712 was used in ELISA to investigate if caspase-dependent apoptosis induction is a mechanism of action of infliximab
|Shen C,Maerten P,Geboes K,Van Assche G,Rutgeerts P,Ceuppens JL||Clinical immunology (Orlando, Fla.) (115:250)||2005|
|Human||Not Cited||Synthetic TLR agonists reveal functional differences between human TLR7 and TLR8.||Gorden KB,Gorski KS,Gibson SJ,Kedl RM,Kieper WC,Qiu X,Tomai MA,Alkan SS,Vasilakos JP||Journal of immunology (Baltimore, Md. : 1950) (174:1259)||2005|
|Not Applicable||Not Cited||
The structure and biological characteristics of the Spirochaeta aurantia outer membrane glycolipid LGLB.
AHC3712 was used in ELISA to isolate and characterize LGLB from Spirochaeta aurantia
|Vinogradov E,Paul CJ,Li J,Zhou Y,Lyle EA,Tapping RI,Kropinski AM,Perry MB||European journal of biochemistry (271:4685)||2004|
Tumor necrosis factor-alpha induces functionally active hyaluronan-adhesive CD44 by activating sialidase through p38 mitogen-activated protein kinase in lipopolysaccharide-stimulated human monocytic cells.
AHC3712 was used in ELISA to suggest that TNF-alpha-induced p38 MAPK activation regulates the induction of functionally active HA-binding form of CD44 by activating sialidase in LPS-stimulated human monocytic cells.
|Gee K,Kozlowski M,Kumar A||The Journal of biological chemistry (278:37275)||2003|
Differential regulation of CD44 expression by lipopolysaccharide (LPS) and TNF-alpha in human monocytic cells: distinct involvement of c-Jun N-terminal kinase in LPS-induced CD44 expression.
AHC3712 was used in ELISA to study CD44 in monocytic cells.
|Gee K,Lim W,Ma W,Nandan D,Diaz-Mitoma F,Kozlowski M,Kumar A||Journal of immunology (Baltimore, Md. : 1950) (169:5660)||2002|
Euglycemic hyperinsulinemia augments the cytokine and endocrine responses to endotoxin in humans.
AHC3712 was used in ELISA to examine the effect of in vivo changes in glucose availability and plasma insulin concentrations in humans.
|Soop M,Duxbury H,Agwunobi AO,Gibson JM,Hopkins SJ,Childs C,Cooper RG,Maycock P,Little RA,Carlson GL||American journal of physiology. Endocrinology and metabolism (282:E1276)||2002|
Igf-I inhibits spontaneous apoptosis in human granulocytes.
AHC3712 was used in ELISA to examine the effects of IGF-I on in vitro apoptosis of human peripheral blood granulocytes.
|Kooijman R,Coppens A,Hooghe-Peters E||Endocrinology (143:1206)||2002|
Activation of the formyl peptide receptor by the HIV-derived peptide T-20 suppresses interleukin-12 p70 production by human monocytes.
AHC3712 was used in ELISA to study the mechanism of HIV-mediated suppression of IL-2 production.
|Braun MC,Wang JM,Lahey E,Rabin RL,Kelsall BL||Blood (97:3531)||2001|
Effects of co-stimulation by CD58 on human T cell cytokine production: a selective cytokine pattern with induction of high IL-10 production.
AHC3712 was used in ELISA to examine the effects of co-stimulation by CD58 on the production of Th1-type or Th2 type cytokines.
|Bullens DM,Rafiq K,Charitidou L,Peng X,Kasran A,Warmerdam PA,Van Gool SW,Ceuppens JL||International immunology (13:181)||2001|
Toll-like receptor 4, but not toll-like receptor 2, is a signaling receptor for Escherichia and Salmonella lipopolysaccharides.
AHC3712 was used in ELISA to study the contribution of TLR2 and TLR4 to LPS-induced signaling.
|Tapping RI,Akashi S,Miyake K,Godowski PJ,Tobias PS||Journal of immunology (Baltimore, Md. : 1950) (165:5780)||2000|
Selective suppression of IL-12 production by chemoattractants.
AHC3712 was used in ELISA to determine the ability of chemoattractants to affect IL-2 production by human monocytes and dendritic cells.
|Braun MC,Lahey E,Kelsall BL||Journal of immunology (Baltimore, Md. : 1950) (164:3009)||2000|
Cholera toxin suppresses interleukin (IL)-12 production and IL-12 receptor beta1 and beta2 chain expression.
AHC3712 was used in ELISA to show that cholera toxin inhibits the production of interleukin-2.
|Braun MC,He J,Wu CY,Kelsall BL||The Journal of experimental medicine (189:541)||1999|
Biocompatibility of electrochemical glucose sensors implanted in the subcutis of pigs.
AHC3712 was used in immunohistochemistry to characterize implanted electrochemical glucose sensors in the subcutis of pigs and their biocompatibility
|Kvist PH,Iburg T,Bielecki M,Gerstenberg M,Buch-Rasmussen T,Hasselager E,Jensen HE||Diabetes technology and therapeutics (8:463)||2006|