|Tested species reactivity||Mouse, Rat|
|Published species reactivity||Mouse|
|Host / Isotype||Rat / IgG1|
|Immunogen||Recombinant, murine TNF alpha expressed in E. coli.|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay dependent|
|Flow Cytometry (Flow)||Assay-dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-20303 detects TNF alpha in mouse and rat samples. Reactivity with mouse TNF beta has not been tested.
MA1-20303 has been successfully used in ELISA procedures.
The MA1-20303 immunogen is recombinant, mouse TNF alpha expressed in E. coli.
Tumor Necrosis Factor-Alpha (TNF alpha) is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor-bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Immunization of mice with phosphatidylcholine drastically reduces the parasitaemia of subsequent Plasmodium chabaudi chabaudi blood-stage infections.
MA1-20303 was used in ELISA to investigate the effect of phosphatidylcholine immunization of mice for the parasitaemia of subsequent Plasmodium chabaudi chabaudi blood-stage infections
|Bordmann G,Rudin W,Favre N||Immunology (94:35)||1998|
Comparison of cytokine measurements using ELISA, ELISPOT and semi-quantitative RT-PCR.
MA1-20303 was used in ELISA to compare the three approaches for cytokine measurements
|Favre N,Bordmann G,Rudin W||Journal of immunological methods (204:57)||1997|
A role for perforin in activation-induced cell death.
MA1-20303 was used in blocking/activating experiment to investigate the function of perforin in cell death
|Spaner D,Raju K,Radvanyi L,Lin Y,Miller RG||Journal of immunology (Baltimore, Md. : 1950) (160:2655)||1998|