Mouse splenocytes were unstimulated (top) or stimulated for 5 hours with Cell Stimulation Cocktail (plus protein transport inhibitors) (bottom), then intracellularly stained with Anti-Mouse CD4 eFluor® 450 and 0.25 ug of Anti-Mouse/Rat TNF alpha PE (A18495). Total viable cells, as determined by Fixable Viability Dye eFluor®780, were used for analysis.
|Tested species reactivity||Mouse, Rat|
|Host / Isotype||Armenian hamster / IgG|
|Immunogen||mouse tumor necrosis factor alpha|
|Storage buffer||proprietary buffer|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.
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