Invitrogen
Applications | Tested Dilution | Publications |
---|---|---|
Immunohistochemistry (Frozen) (IHC (F)) |
- | View 1 publication 1 publication |
ELISA (ELISA) |
1-4 µg/mL | View 13 publications 13 publications |
Functional Assay (FN) |
- | View 1 publication 1 publication |
T-Cell Activation (TCA) |
- | View 1 publication 1 publication |
Product Specifications | |
---|---|
Species Reactivity |
Mouse |
Published species |
Mouse |
Host/Isotype |
Rat / IgM, kappa |
Class |
Monoclonal |
Type |
Antibody |
Clone |
1F3F3D4 |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
0.5 mg/mL |
Purification |
Affinity chromatography |
Storage buffer |
PBS, pH 7.2 |
Contains |
0.09% sodium azide |
Storage conditions |
4° C |
RRID |
AB_468481 |
Description: The 1F3F3D4 antibody reacts with mouse tumor necrosis factor-alpha (TNFaalpha), a 17 kDa cytokine produced by monocytes, macrophages, neutrophils, NK cells and CD4(+) T cells.
Applications Reported: The 1F3F3D4 antibody has been reported for use in capture of mouse TNF alpha by ELISA and ELISPOT.
Applications Tested: The 1F3F3D4 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of mouse TNF alpha (Tumor Necrosis Factor alpha). A suitable range of concentrations of this antibody for ELISA capture is 1-4 µg/mL.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: C-domain 1; C-domain 2; Cachectin; DADB-70P7.1; ICD1; ICD2; Intracellular domain 1; Intracellular domain 2; N-terminal fragment; NTF; TNF alpha; TNF superfamily; TNF α; TNF-a; TNF-alpha; TNFα; Tumor necrosis factor; Tumor necrosis factor ligand superfamily member 2; Tumor necrosis factor, membrane form; Tumor necrosis factor, soluble form; tumor necrosis factor-alpha
Gene Aliases: DIF; Tnf; TNF-a; TNF-alpha; Tnfa; TNFalpha; Tnfsf1a; TNFSF2
UniProt ID: (Mouse) P06804
Entrez Gene ID: (Mouse) 21926
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