|ELISA (ELISA)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
|Tested Species reactivity||Bovine|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Recombinant bovine TNF alpha purified from Pichia pastoris|
|Storage buffer||PBS with 5% trehalose|
|Storage conditions||4° C|
MBOTNFAI targets TNF Alpha in ELISA, and WB applications and shows reactivity with Bovine samples.
The MBOTNFAI immunogen is recombinant bovine TNFa purified from Pichia pastoris.
MBOTNFAI detects TNF Alpha which has a predicted molecular weight of approximately 26 kDa.
The MBOTNFAI bovine TNFa monoclonal antibody (clone E10011) has successfully been paired as the coating antibody in a sandwich ELISA with biotinylated polyclonal detection antibody PBOTNFABI . Typical dilutions for sandwich ELISA include 1 µg/ml for coating and 0.125 - 0.25 µg/ml for detection.
This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Cachectin; TNF-a; TNF-alpha; Tumor necrosis factor; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor, alpha (TNF superfamily, member 2)
Gene Aliases: BOS_21587; TNF; TNF-a; TNF-alpha; TNFA; TNFSF2
UniProt ID: (Bovine) Q06599
Entrez Gene ID: (Bovine) 280943
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