Normal human blood peripheral cells were stimulated with PMA/Ionomycin in the presence of Brefeldin A (Product # 00-4506) for 6 hours. Unstimulated (left) or stimulated (right) cells were surface stained with Anti-Human CD3 PE (Product # 12-0038) and intracellularly stained with Anti-Human TNF alpha FITC.
|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG1, kappa|
|Storage buffer||PBS, pH 7.2, with 0.1% gelatin|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C, store in dark, DO NOT FREEZE!|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1 µg/test|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Description: The MAb11 antibody reacts with human tumor necrosis factor-alpha (TNF alpha), a 17 kDa cytokine produced by monocytes, macrophages, neutrophils, NK cells and CD4+ T cells. TN alpha has cytolytic activity against a range of tumor cells and is important in immune regulation. TNF alpha forms dimers and trimers and also exists as a 26 kDa membrane bound form. The MAb11 antibody is a neutralizing antibody.
Applications Reported: Mab11 has been reported for use in intracellular flow cytometric analysis.
Applications Tested: This MAb11 antibody is offered in 2 formats: - µg size: has been tested by intracellular staining and flow cytometric analysis of stimulated human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. - test size: has been pre-titrated and tested by intracellular staining and flow cytometric analysis of stimulated human peripheral blood cells. Refer to catalog number suffix on the vial for amount to use per test: -41 is 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser
Tumor Necrosis Factor-Alpha (TNF alpha) is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor-bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single ~17 kDa band following SDS-PAGE under non-reducing conditions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.