Invitrogen

TNF alpha Monoclonal Antibody (MAb11), Alexa Fluor™ 700, eBioscience™

29 Published Figures

31 References

View all (220) TNF alpha antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (29)

TNF alpha Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 30

TNF alpha Antibody (56-7349-42) in Flow

Normal human peripheral blood cells were stimulated for 5 hours with PMA/ionomycin and Brefeldin A (right) or unstimulated (left), followed by intracellular staining with Anti-Human CD4 FITC (Product # 11-0048-42) and Anti-Human TNF alpha Alexa Fluor® 700 using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Cells in the lymphocyte gate were used for analysis.

TNF alpha Antibody in Immunohistochemistry (IHC)

TNF alpha Antibody in Flow Cytometry, Neutralization (Flow, Neu)

Product Image Gallery

Product Details

56-7349-42

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Flow Cytometry (Flow)

5 µL (0.25 µg)/test

View 28 publications 28 publications

Neutralization (Neu)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse, Rhesus monkey

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Alexa Fluor™ 700, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

MAb11

Conjugate

Alexa Fluor™ 700 Alexa Fluor™ 700 Alexa Fluor™ 700

Excitation/Emission Max

696/719 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with BSA

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_10671335

Product Specific Information

Description: The MAb11 antibody reacts with human tumor necrosis factor-alpha (TNF alpha), a 17 kDa cytokine produced by monocytes, macrophages, neutrophils, NK cells and CD4+ T cells. TN alpha has cytolytic activity against a range of tumor cells and is important in immune regulation. TNF alpha forms dimers and trimers and also exists as a 26 kDa membrane bound form. The MAb11 antibody is a neutralizing antibody.

Applications Reported: This MAb11 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This MAb11 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of stimulated normal human peripheral blood cells using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Please refer to BestProtocols®: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Alexa Fluor® 700 emits at 723 nm and can be excited with the red laser (633 nm). Most instruments will require a 685 LP mirror and 710/20 filter. Please make sure that your instrument is capable of detecting this fluorochrome.

Excitation: 633-647 nm; Emission: 723 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

The effect of metformin on senescence of T lymphocytes.

Immunity & ageing : I & A

Yang J,Liu HC,Zhang JQ,Zou JY,Zhang X,Chen WM,Gu Y,Hong H

Published figure using TNF alpha monoclonal antibody (Product # 56-7349-42) in Flow Cytometry

Tue Dec 12 00:00:00 UTC 2023

Dynamics of humoral and cellular response to three doses of anti-SARS-CoV-2 BNT162b2 vaccine in patients with hematological malignancies and older subjects.

Frontiers in immunology

Laquintana V,Mottini C,Marchesi F,Marcozzi B,Terrenato I,Sperandio E,de Latouliere L,Carrieri F,Pimpinelli F,Pontone M,Pellini R,Campo F,Conti L,Accetta C,Mandoj C,Petrone F,Di Bella O,Vujovic B,Morrone A,Compagnone M,Principato E,Pinto E,Papa E,Falcucci P,La Malfa A,Pallocca M,De Marco F,Piaggio G,Ciliberto G,Mengarelli A,di Martino S

Published figure using TNF alpha monoclonal antibody (Product # 56-7349-42) in Flow Cytometry

Wed Feb 14 00:00:00 UTC 2024

R274X-mutated Phf6 increased the self-renewal and skewed T cell differentiation of hematopoietic stem cells.

iScience

Lan Y,Yuan S,Guo T,Hou S,Zhao F,Yang W,Cao Y,Chu Y,Jiang E,Yuan W,Wang X

Published figure using TNF alpha monoclonal antibody (Product # 56-7349-42) in Flow Cytometry

Fri Jun 16 00:00:00 UTC 2023

An epitope encoded by uORF of RNF10 elicits a therapeutic anti-tumor immune response.

Molecular therapy oncolytics

Zeng L,Zheng W,Zhang J,Wang J,Ji Q,Wu X,Meng Y,Zhu X

Published figure using TNF alpha monoclonal antibody (Product # 56-7349-42) in Flow Cytometry

Tue Dec 19 00:00:00 UTC 2023

LINC01123 promotes immune escape by sponging miR-214-3p to regulate B7-H3 in head and neck squamous-cell carcinoma.

Cell death & disease

Li H,Yang Z,Yang X,Zhang F,Wang J,Wu Z,Wanyan C,Meng Q,Gao W,Yang X,Wei J

Published figure using TNF alpha monoclonal antibody (Product # 56-7349-42) in Flow Cytometry

Thu Feb 03 00:00:00 UTC 2022

PRDM1/BLIMP1 induces cancer immune evasion by modulating the USP22-SPI1-PD-L1 axis in hepatocellular carcinoma cells.

Nature communications

Li Q,Zhang L,You W,Xu J,Dai J,Hua D,Zhang R,Yao F,Zhou S,Huang W,Dai Y,Zhang Y,Baheti T,Qian X,Pu L,Xu J,Xia Y,Zhang C,Tang J,Wang X

Published figure using TNF alpha monoclonal antibody (Product # 56-7349-42) in Flow Cytometry

Mon Dec 12 00:00:00 UTC 2022

Enhanced antibody responses in fully vaccinated individuals against pan-SARS-CoV-2 variants following Omicron breakthrough infection.

Cell reports. Medicine

Jeong HW,Kim SM,Jung MK,Noh JY,Yoo JS,Kim EH,Kim YI,Yu K,Jang SG,Gil J,Casel MA,Rare R,Choi JH,Kim HS,Kim JH,Um J,Kim C,Kim Y,Chin BS,Jung S,Choi JY,Song KH,Kim YD,Park JS,Song JY,Shin EC,Choi YK

Published figure using TNF alpha monoclonal antibody (Product # 56-7349-42) in Flow Cytometry

Tue Oct 18 00:00:00 UTC 2022

Baricitinib treatment resolves lower-airway macrophage inflammation and neutrophil recruitment in SARS-CoV-2-infected rhesus macaques.

Cell

Hoang TN,Pino M,Boddapati AK,Viox EG,Starke CE,Upadhyay AA,Gumber S,Nekorchuk M,Busman-Sahay K,Strongin Z,Harper JL,Tharp GK,Pellegrini KL,Kirejczyk S,Zandi K,Tao S,Horton TR,Beagle EN,Mahar EA,Lee MYH,Cohen J,Jean SM,Wood JS,Connor-Stroud F,Stammen RL,Delmas OM,Wang S,Cooney KA,Sayegh MN,Wang L,Filev PD,Weiskopf D,Silvestri G,Waggoner J,Piantadosi A,Kasturi SP,Al-Shakhshir H,Ribeiro SP,Sekaly RP,Levit RD,Estes JD,Vanderford TH,Schinazi RF,Bosinger SE,Paiardini M

Published figure using TNF alpha monoclonal antibody (Product # 56-7349-42) in Flow Cytometry

Thu Jan 21 00:00:00 UTC 2021

Membrane lymphotoxin-α₂β is a novel tumor necrosis factor (TNF) receptor 2 (TNFR2) agonist.

Cell death & disease

Kucka K,Lang I,Zhang T,Siegmund D,Medler J,Wajant H

Published figure using TNF alpha monoclonal antibody (Product # 56-7349-42) in Flow Cytometry

Tue Apr 06 00:00:00 UTC 2021

NKD2 mediates stimulation-dependent ORAI1 trafficking to augment Ca²⁺ entry in T cells.

Cell reports

Wu B,Woo JS,Vila P,Jew M,Leung J,Sun Z,Srikanth S,Gwack Y

Published figure using TNF alpha monoclonal antibody (Product # 56-7349-42) in Flow Cytometry

Tue Aug 24 00:00:00 UTC 2021

Development of an Inflammatory CD14⁺ Dendritic Cell Subset in Humanized Mice.

Frontiers in immunology

Iwabuchi R,Ide K,Terahara K,Wagatsuma R,Iwaki R,Matsunaga H,Tsunetsugu-Yokota Y,Takeyama H,Takahashi Y

Published figure using TNF alpha monoclonal antibody (Product # 56-7349-42) in Flow Cytometry

Mon Sep 27 00:00:00 UTC 2021

Cellular and humoral immune responses following SARS-CoV-2 mRNA vaccination in patients with multiple sclerosis on anti-CD20 therapy.

Nature medicine

Apostolidis SA,Kakara M,Painter MM,Goel RR,Mathew D,Lenzi K,Rezk A,Patterson KR,Espinoza DA,Kadri JC,Markowitz DM,E Markowitz C,Mexhitaj I,Jacobs D,Babb A,Betts MR,Prak ETL,Weiskopf D,Grifoni A,Lundgreen KA,Gouma S,Sette A,Bates P,Hensley SE,Greenplate AR,Wherry EJ,Li R,Bar-Or A

Published figure using TNF alpha monoclonal antibody (Product # 56-7349-42) in Flow Cytometry

Mon Nov 01 00:00:00 UTC 2021

Effects of persistent modulation of intestinal microbiota on SIV/HIV vaccination in rhesus macaques.

NPJ vaccines

Klatt NR,Broedlow C,Osborn JM,Gustin AT,Dross S,O'Connor MA,Coronado E,Barnette P,Hensley-McBain T,Zevin AS,Muir R,Roederer A,Wangari S,Iwayama N,Ahrens CY,Smedley J,Moats C,Lynch RM,Haddad EK,Haigwood NL,Fuller DH,Manuzak JA

56-7349-42 was used in Flow Cytometry to evaluate whether probiotic therapy could improve the immunogenicity and protective efficacy of SIV/HIV vaccination.

Thu Mar 11 00:00:00 UTC 2021

Rejuvenating Effector/Exhausted CAR T Cells to Stem Cell Memory-Like CAR T Cells By Resting Them in the Presence of CXCL12 and the NOTCH Ligand.

Cancer research communications

Ando M,Kondo T,Tomisato W,Ito M,Shichino S,Srirat T,Mise-Omata S,Nakagawara K,Yoshimura A

Published figure using TNF alpha monoclonal antibody (Product # 56-7349-42) in Flow Cytometry

Fri Oct 01 00:00:00 UTC 2021

Implanted pluripotent stem-cell-derived pancreatic endoderm cells secrete glucose-responsive C-peptide in patients with type 1 diabetes.

Cell stem cell

Ramzy A,Thompson DM,Ward-Hartstonge KA,Ivison S,Cook L,Garcia RV,Loyal J,Kim PTW,Warnock GL,Levings MK,Kieffer TJ

56-7349-42 was used in flow cytometry to report an analysis on 1 year of data from the first cohort of 15 patients from a single trial site that received subcutaneous implantation of cell products combined with an immunosuppressive regimen.

Thu Dec 02 00:00:00 UTC 2021

Inhibition of Cholesterol Esterification Enzyme Enhances the Potency of Human Chimeric Antigen Receptor T Cells against Pancreatic Carcinoma.

Molecular therapy oncolytics

Zhao L,Liu Y,Zhao F,Jin Y,Feng J,Geng R,Sun J,Kang L,Yu L,Wei Y

Published figure using TNF alpha monoclonal antibody (Product # 56-7349-42) in Flow Cytometry

Fri Mar 27 00:00:00 UTC 2020

Abrogation of HLA surface expression using CRISPR/Cas9 genome editing: a step toward universal T cell therapy.

Scientific reports

Lee J,Sheen JH,Lim O,Lee Y,Ryu J,Shin D,Kim YY,Kim M

Published figure using TNF alpha monoclonal antibody (Product # 56-7349-42) in Flow Cytometry

Tue Oct 20 00:00:00 UTC 2020

Ghrelin ameliorates tumor-induced adipose tissue atrophy and inflammation via Ghrelin receptor-dependent and -independent pathways.

Oncotarget

Liu H,Luo J,Guillory B,Chen JA,Zang P,Yoeli JK,Hernandez Y,Lee II,Anderson B,Storie M,Tewnion A,Garcia JM

Published figure using TNF alpha monoclonal antibody (Product # 56-7349-42) in Immunohistochemistry

Tue Sep 01 00:00:00 UTC 2020

Mucosal-associated invariant T (MAIT) cells mediate protective host responses in sepsis.

eLife

Trivedi S,Labuz D,Anderson CP,Araujo CV,Blair A,Middleton EA,Jensen O,Tran A,Mulvey MA,Campbell RA,Hale JS,Rondina MT,Leung DT

Published figure using TNF alpha monoclonal antibody (Product # 56-7349-42) in Flow Cytometry

Mon Nov 09 00:00:00 UTC 2020

CAIX Regulates GBM Motility and TAM Adhesion and Polarization through EGFR/STAT3 under Hypoxic Conditions.

International journal of molecular sciences

Huang BR,Liu YS,Lai SW,Lin HJ,Shen CK,Yang LY,Lu DY

Published figure using TNF alpha monoclonal antibody (Product # 56-7349-42) in Flow Cytometry

Fri Aug 14 00:00:00 UTC 2020

CD4⁺ T Cells Cross-Reactive with Dengue and Zika Viruses Protect against Zika Virus Infection.

Cell reports

Wen J,Wang YT,Valentine KM,Dos Santos Alves RP,Xu Z,Regla-Nava JA,Ngono AE,Young MP,Ferreira LCS,Shresta S

56-7349 was used in Flow cytometry/Cell sorting to examine the ability of DENV/ZIKV-cross-reactive CD4+ T cells to protect against versus exacerbate ZIKV infection.

Tue Apr 28 00:00:00 UTC 2020

Heterogeneous Tumor-Immune Microenvironments among Differentially Growing Metastases in an Ovarian Cancer Patient.

Cell

Jiménez-Sánchez A,Memon D,Pourpe S,Veeraraghavan H,Li Y,Vargas HA,Gill MB,Park KJ,Zivanovic O,Konner J,Ricca J,Zamarin D,Walther T,Aghajanian C,Wolchok JD,Sala E,Merghoub T,Snyder A,Miller ML

Published figure using TNF alpha monoclonal antibody (Product # 56-7349-42) in Flow Cytometry

Thu Aug 24 00:00:00 UTC 2017

The distribution and function of human memory T cell subsets in lung cancer.

Immunologic research

Sheng SY,Gu Y,Lu CG,Zou JY,Hong H,Wang R

Published figure using TNF alpha monoclonal antibody (Product # 56-7349-42) in Flow Cytometry

Thu Jun 01 00:00:00 UTC 2017

Application of a whole blood mycobacterial growth inhibition assay to study immunity against Mycobacterium tuberculosis in a high tuberculosis burden population.

PloS one

Baguma R,Penn-Nicholson A,Smit E,Erasmus M,Day J,Makhethe L,de Kock M,Hughes EJ,van Rooyen M,Pienaar B,Stone L,Hanekom W,Brennan MJ,Wallis RS,Hatherill M,Scriba TJ

Published figure using TNF alpha monoclonal antibody (Product # 56-7349-42) in Flow Cytometry

Wed Oct 18 00:00:00 UTC 2017

Human Mucosa-Associated Invariant T Cells Accumulate in Colon Adenocarcinomas but Produce Reduced Amounts of IFN-γ.

Journal of immunology (Baltimore, Md. : 1950)

Sundström P,Ahlmanner F,Akéus P,Sundquist M,Alsén S,Yrlid U,Börjesson L,Sjöling Å,Gustavsson B,Wong SB,Quiding-Järbrink M

56-7349 was used in Flow cytometry/Cell sorting to elucidate the presence and function of mucosa-associated invariant T cells in tumours.

Thu Oct 01 00:00:00 UTC 2015

Targeting aPKC disables oncogenic signaling by both the EGFR and the proinflammatory cytokine TNFα in glioblastoma.

Science signaling

Kusne Y,Carrera-Silva EA,Perry AS,Rushing EJ,Mandell EK,Dietrich JD,Errasti AE,Gibbs D,Berens ME,Loftus JC,Hulme C,Yang W,Lu Z,Aldape K,Sanai N,Rothlin CV,Ghosh S

Published figure using TNF alpha monoclonal antibody (Product # 56-7349-42) in Flow Cytometry

Tue Aug 12 00:00:00 UTC 2014

Targeting aPKC disables oncogenic signaling by both the EGFR and the proinflammatory cytokine TNFα in glioblastoma.

Science signaling

Kusne Y,Carrera-Silva EA,Perry AS,Rushing EJ,Mandell EK,Dietrich JD,Errasti AE,Gibbs D,Berens ME,Loftus JC,Hulme C,Yang W,Lu Z,Aldape K,Sanai N,Rothlin CV,Ghosh S

Published figure using TNF alpha monoclonal antibody (Product # 56-7349-42) in Flow Cytometry

Tue Aug 12 00:00:00 UTC 2014

Targeting aPKC disables oncogenic signaling by both the EGFR and the proinflammatory cytokine TNFα in glioblastoma.

Science signaling

Kusne Y,Carrera-Silva EA,Perry AS,Rushing EJ,Mandell EK,Dietrich JD,Errasti AE,Gibbs D,Berens ME,Loftus JC,Hulme C,Yang W,Lu Z,Aldape K,Sanai N,Rothlin CV,Ghosh S

Published figure using TNF alpha monoclonal antibody (Product # 56-7349-42) in Flow Cytometry

Tue Aug 12 00:00:00 UTC 2014

Targeting aPKC disables oncogenic signaling by both the EGFR and the proinflammatory cytokine TNFα in glioblastoma.

Science signaling

Kusne Y,Carrera-Silva EA,Perry AS,Rushing EJ,Mandell EK,Dietrich JD,Errasti AE,Gibbs D,Berens ME,Loftus JC,Hulme C,Yang W,Lu Z,Aldape K,Sanai N,Rothlin CV,Ghosh S

Published figure using TNF alpha monoclonal antibody (Product # 56-7349-42) in Flow Cytometry

Tue Aug 12 00:00:00 UTC 2014

Cell-mediated immune responses to respiratory syncytial virus infection: magnitude, kinetics, and correlates with morbidity and age.

Human vaccines & immunotherapeutics

Geevarghese B,Weinberg A

Published figure using TNF alpha monoclonal antibody (Product # 56-7349-42) in Flow Cytometry

Mon Apr 25 00:00:00 UTC 2016

Association of a NOD2 gene polymorphism and T-helper 17 cells with presumed ocular toxoplasmosis.

The Journal of infectious diseases

Dutra MS,Béla SR,Peixoto-Rangel AL,Fakiola M,Cruz AG,Gazzinelli A,Quites HF,Bahia-Oliveira LM,Peixe RG,Campos WR,Higino-Rocha AC,Miller NE,Blackwell JM,Antonelli LR,Gazzinelli RT

56-7349 was used in Flow cytometry/Cell sorting to suggest that NOD2 influences the production of IL-17A by CD4 T lymphocytes and might contribute to the development of ocular toxoplasmosis.

Tue Jan 01 00:00:00 UTC 2013

Functional OCT4-specific CD4⁺ and CD8⁺ T cells in healthy controls and ovarian cancer patients.

Oncoimmunology

Di J,Massuger LF,Duiveman-de Boer T,Zusterzeel PL,Figdor CG,Torensma R

Published figure using TNF alpha monoclonal antibody (Product # 56-7349-42) in Flow Cytometry

Wed May 01 00:00:00 UTC 2013

Bioinformatics

Protein Aliases: APC1 protein; Cachectin; DADB-70P7.1; TNF alpha; TNF superfamily; TNF α; TNF, macrophage-derived; TNF, monocyte-derived; TNF-a; TNF-alpha; TNFα; Tumor necrosis factor; tumor necrosis factor ligand 1F; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor-alpha

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Gene Aliases: DIF; TNF; TNF-alpha; TNFA; TNFSF2; TNLG1F

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UniProt ID: (Human) P01375

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Entrez Gene ID: (Human) 7124

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Function(s)

protease binding cytokine activity tumor necrosis factor receptor binding protein binding identical protein binding transcription regulatory region DNA binding

Process(es)

Performance Guarantee

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TNF alpha Monoclonal Antibody (MAb11), Alexa Fluor™ 700, eBioscience™

TNF alpha Antibody (56-7349-42) in Flow

Product Details

56-7349-42

Immunohistochemistry (IHC)

Flow Cytometry (Flow)

Neutralization (Neu)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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