|Tested species reactivity||Human|
|Published species reactivity||Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Storage buffer||PBS, pH 7.2, with 0.1% gelatin|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C, store in dark, DO NOT FREEZE!|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||0.125 µg/test|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Description: The 359-81-11 antibody reacts with Tumor Necrosis Factor-beta (TNF-beta), a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor cells and other target cells.
Applications Reported: The 359-81-11 antibody has been reported for use in detection of human TNF beta by ELISA and intracellular staining for flow cytometric analysis.
Applications Tested: This 359-81-11 antibody is offered in 2 formats: - µg size: has been tested by intracellular staining and flow cytometric analysis of TH1 polarized restimulated normal human peripheral blood cells. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. - test size: has been pre-titrated and tested by intracellular staining and flow cytometric analysis of TH1 polarized restimulated normal human peripheral blood cells. This can be used at test size: 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser
The encoded protein, a member of the tumor necrosis factor family, is a cytokine produced by lymphocytes. The protein is highly inducible, secreted, and forms heterotrimers with lymphotoxin-beta which anchor lymphotoxin-alpha to the cell surface. This protein also mediates a large variety of inflammatory, immunostimulatory, and antiviral responses, is involved in the formation of secondary lymphoid organs during development and plays a role in apoptosis. Genetic variations in this gene are associated with susceptibility to leprosy type 4 and psoriatic arthritis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||Distinct profiles of human B cell effector cytokines: a role in immune regulation?||Duddy ME,Alter A,Bar-Or A||Journal of immunology (Baltimore, Md. : 1950) (172:3422)||2004|
|Not Applicable||Not Cited||TCRalphabeta+/CD4+ large granular lymphocytosis: a new clonal T-cell lymphoproliferative disorder.||Lima M,Almeida J,Dos Anjos Teixeira M,Alguero Md Mdel C,Santos AH,Balanzategui A,Queirós ML,Bárcena P,Izarra A,Fonseca S,Bueno C,Justiça B,Gonzalez M,San Miguel JF,Orfao A||The American journal of pathology (163:763)||2003|
|Not Applicable||Not Cited||Tumor eradication by hepatitis B virus X antigen-specific CD8+ T cells in xenografted nude mice.||Chun E,Lee J,Cheong HS,Lee KY||Journal of immunology (Baltimore, Md. : 1950) (170:1183)||2003|
|Not Applicable||Not Cited||Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples.||Abrams JS,Roncarolo MG,Yssel H,Andersson U,Gleich GJ,Silver JE||Immunological reviews (127:5)||1992|