Staining of Th1-polarized normal human peripheral blood cells with Anti-Human CD4 FITC followed by fixation, permeabilization and intracellular staining with 0.125 ug of Mouse IgG1 kappa Isotype Control PE (top) or Anti-Human TNF beta PE (bottom, A18494). Total viable cells were used for analysis.
|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||human tumor necrosis factor beta (TNF beta)|
|Storage buffer||proprietary buffer|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
The encoded protein, a member of the tumor necrosis factor family, is a cytokine produced by lymphocytes. The protein is highly inducible, secreted, and forms heterotrimers with lymphotoxin-beta which anchor lymphotoxin-alpha to the cell surface. This protein also mediates a large variety of inflammatory, immunostimulatory, and antiviral responses, is involved in the formation of secondary lymphoid organs during development and plays a role in apoptosis. Genetic variations in this gene are associated with susceptibility to leprosy type 4 and psoriatic arthritis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.