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Invitrogen
TNF beta Chimeric Recombinant Rabbit Monoclonal Antibody (3F12.2D3)
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TNF beta Chimeric Antibody (MA5-48071) in WB
Western Blot analysis of TNF beta was performed using TNF beta Chimeric Monoclonal Antibody (Product # MA5-48071). Lipopolysaccharide(LPS)-treated human peripheral blood monocyte (PBM) lysate (35 µg protein in RIPA buffer) was resolved on a 15% SDS PAGE gel and blots probed with TNF beta Chimeric Monoclonal Antibody at 2 µg/mL before detection using an anti-rabbit secondary antibody. Protein was detected by chemiluminescence. The expected unmodified running size for Lymphotoxin alpha is 22.3 kDa, though the form shown here is likely glycos... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-48071
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG, kappa
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
3F12.2D3
Immunogen
3F12 was prepared by hyperimmunizing BALB/c mice with purified recombinant human LT-alpha expressed in E. coli.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
200 µg
Purification
Protein A
Storage buffer
PBS
Contains
0.02% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Specificity: 3F12 binds to human LT-alpha-3 and LT-alpha-Beta in both its soluble homotrimeric and membrane heterotrimeric forms.
Preparation: 3F12 was prepared by hyperimmunizing BALB/c mice with purified recombinant human LTalpha expressed in E. coli - the hybridoma clone was selected for its ability to bind LTalpha3 by ELISA. The CDRs of the antibody were grafted to the framework regions of the mice mAb 2G7 to create the chimeric version of the antibody.
Target Information
TNF beta, a member of the tumor necrosis factor family, is a cytokine produced by lymphocytes. The protein is highly inducible, secreted, and forms heterotrimers with lymphotoxin-beta which anchor lymphotoxin-alpha to the cell surface. This protein also mediates a large variety of inflammatory, immunostimulatory, and antiviral responses, is involved in the formation of secondary lymphoid organs during development and plays a role in apoptosis. Genetic variations in this gene are associated with susceptibility to leprosy type 4 and psoriatic arthritis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DAMA-25N12.13; hypothetical protein; lymphotoxin alpha isoform b; hypothetical protein; lymphotoxin alpha isoform c; hypothetical protein; lymphotoxin alpha isoform d; hypothetical protein; lymphotoxin alpha isoform e; hypothetical protein; lymphotoxin alpha isoform f; hypothetical protein; lymphotoxin alpha isoform g; LT-alpha; LTA; TNF b; TNF beta; TNF superfamily, member 1; TNF β; TNF-beta; TNFbeta; TNFβ; tumor necrosis factor beta; tumor necrosis factor ligand 1E; tumor necrosis factor ligand superfamily member 1; unnamed protein product
Gene Aliases: LT; TNFB; TNFSF1; TNLG1E
UniProt ID: (Human) P01374
Entrez Gene ID: (Human) 4049
response to hypoxia
apoptotic process
immune response
cell communication
signal transduction
cell surface receptor signaling pathway
activation of NF-kappaB-inducing kinase activity
cell-cell signaling
response to nutrient
response to xenobiotic stimulus
signaling
regulation of type I interferon production
response to lipopolysaccharide
dendritic cell homeostasis
positive regulation of apoptotic process
positive regulation of I-kappaB kinase/NF-kappaB signaling
negative regulation of fibroblast proliferation
positive regulation of glial cell proliferation
regulation of necroptotic process
positive regulation of extrinsic apoptotic signaling pathway
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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