OriGene

TNFSF4 Monoclonal Antibody (OTI5F10), TrueMAB™

View all (17) OX40L (CD252) antibodies

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TNFSF4 Antibody in Western Blot (WB) — HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TNFSF4 (RC224021, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-TNFSF. Positive lysates LY401143 (100 µg) and LC401143 (20 µg) can be purchased separately from OriGene.

Product Details

Tested Applications

Dilution

Western Blot (WB)

1:150

Product Specifications

Species Reactivity

Human

Host / Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

OTI5F10

Immunogen

Full length human recombinant protein of human TNFSF4 produced in HEK293T cell.

Conjugate

Unconjugated

Form

liquid

Purification

Affinity chromatography

Storage buffer

PBS with 1% BSA, 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Target Information

The tumor necrosis factor superfamily member TNFSF4 is a type II membrane bound, non-covalently linked homotrimeric protein. It is expressed on antigen presenting cells, such as dendritic cells and activated B-cells, and also on other cells such as vascular endothelial cells, mast cells, and natural killer cells. TNFSF4 signals specifically through the TNFRSF4 receptor, is expressed predominantly on CD4+T cells but also on certain activated CD8+T cells. TNFRSF4/TNFSF4 functions as a costimulatory signal, which is required for a productive interaction between antigen presenting cells and their target T-cells. It enhances cell proliferation and survival, and increases expression of RANTES, IL-2, IL-3, and IFNgamma. TNFRSF4/TNFSF4 signaling plays an important role in immunotolerance.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: CD134 ligand; CD134L; CD252; Glycoprotein Gp34; GP34; OX-40L; OX40 antigen ligand; OX40 ligand; OX40L; OX4OL; TAX transcriptionally-activated glycoprotein 1; tax-transcriptionally activated glycoprotein 1 (34kD); TNLG2B; tumor necrosis factor (ligand) superfamily member 4; tumor necrosis factor ligand 2B; Tumor necrosis factor ligand superfamily member 4; TXGP1

Gene Aliases: CD134L; CD252; GP34; OX-40L; OX4OL; TNFSF4; TNLG2B; TXGP1

UniProt ID: (Human) P23510

Entrez Gene ID: (Human) 7292

Molecular Function: cytokine signaling molecule tumor necrosis factor family member

defense response to nematode acute inflammatory response positive regulation of T cell cytokine production regulation of adaptive immune response positive regulation of type 2 immune response positive regulation of immunoglobulin mediated immune response immune response signal transduction cholesterol metabolic process positive regulation of cell proliferation response to virus negative regulation of interferon-gamma production negative regulation of interleukin-17 production positive regulation of interferon-gamma production positive regulation of interleukin-10 production positive regulation of interleukin-12 production positive regulation of interleukin-13 production positive regulation of interleukin-2 production positive regulation of interleukin-4 production positive regulation of interleukin-6 production tumor necrosis factor-mediated signaling pathway memory T cell activation T-helper 2 cell activation response to nitrogen dioxide cellular response to nitrogen dioxide CD4-positive, alpha-beta T cell costimulation T cell proliferation positive regulation of T cell proliferation positive regulation of activated T cell proliferation positive regulation of CD4-positive, alpha-beta T cell differentiation positive regulation of memory T cell differentiation negative regulation of sequence-specific DNA binding transcription factor activity negative regulation of regulatory T cell differentiation negative regulation of T-helper 1 cell differentiation positive regulation of T-helper 2 cell differentiation negative regulation of transcription, DNA-templated positive regulation of alpha-beta T cell proliferation negative regulation of cytokine secretion regulation of inflammatory response positive regulation of inflammatory response positive regulation of B cell activation positive regulation of immunoglobulin secretion cellular response to lipopolysaccharide cellular response to prostaglandin E stimulus chemokine (C-C motif) ligand 11 production positive regulation of CD4-positive, alpha-beta T cell costimulation positive regulation of memory T cell activation positive regulation of T-helper 2 cell activation positive regulation of interleukin-4-dependent isotype switching to IgE isotypes

Process(es)

receptor binding cytokine activity tumor necrosis factor receptor binding tumor necrosis factor receptor superfamily binding